H9c2 cells were plated into a 96-well plate at a density of 1x104 cells/well. Following H/R stimulation and indicated transfection, 10 µl Cell Counting Kit-8 (CCK-8) solution (Sangon Biotech Co., Ltd.) was added to each well and incubated for 3 h at 37˚C. The absorbance was detected at a wavelength of 450 nm using a microplate reader (BioTek Instruments, Inc.) to evaluate the viability of H9c2 cells.
Cell counting kit 8 cck 8 solution
Manufactured by Sangon
The Cell Counting Kit-8 (CCK-8) solution is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes the highly water-soluble tetrazolium salt WST-8 to produce a water-soluble formazan dye upon reduction in the presence of an electron carrier. The amount of the formazan dye generated is directly proportional to the number of living cells.
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Lab products found in correlation
2 protocols using cell counting kit 8 cck 8 solution
1
Evaluating H9c2 Cell Viability
2
Cell Viability Assay with CCK-8
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Cells were cultured in 24-well plates (2 × 10 4 cells/well) for 24 h and incubated with Cell Counting Kit-8 (CCK-8) solution (10 μl; Sangon) at 37°C for 3 h. Absorbance at 450 nm was subsequently analyzed.
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