BALB/c and Rag2−/− mice were purchased from Jackson Laboratory. All animal studies were performed according to NIH guidelines for the use and care of live animals and were approved by the NIAMS Institutional Animal Care and Use Committee. JAKinibs were resuspended in 0.5% methyl cellulose and animals were dosed orally twice daily with vehicle or 30 mg/kg of tofacitinib (kindly provided by Pfizer) or 20 mg/kg of PF-06651600 (provided by the National Center for Advancing Translational Sciences (NCATS), NIH) for 1 week (or 3 days, where indicated) (27 (link)). ABT-199 (Venetoclax, Selleckchem) was resuspended in 60% Phosal 50PG, 30% PEG 400, and 10% EtOH. Animals were dosed orally once a day with vehicle or 90 mg/kg for a week.
Pf 06651600
Manufactured by Pfizer
PF-06651600 is a laboratory equipment product manufactured by Pfizer. It is designed for use in scientific research and analysis. The core function of PF-06651600 is to provide a reliable and precise tool for data collection and measurement. Further details about its intended use or specific applications are not available.
Automatically generated - may contain errors
Lab products found in correlation
Cd34 microbead, by Miltenyi Biotec (2 mentions)
Stem cell factor (scf), by Thermo Fisher Scientific (2 mentions)
Interleukin 7 (il 7), by Thermo Fisher Scientific (2 mentions)
Il 15, by Thermo Fisher Scientific (2 mentions)
Tofacitinib, by Merck Group (2 mentions)
Venetoclax, by Selleck Chemicals (1 mentions)
Rag2 mice, by Jackson ImmunoResearch (1 mentions)
Tofacitinib, by Pfizer (1 mentions)
Balb c, by Jackson ImmunoResearch (1 mentions)
3 protocols using pf 06651600
1
JAKinibs and Venetoclax Administration in Mice
2
Isolation and Culture of Lymphocytes
3
Isolation and Culture of Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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From mice, siLP lymphocytes were isolated by Collagenase 4 digestion after two EDTA washes to remove intraepithelial lymphocytes. Lymphocytes were enriched from digested cells by Percoll gradients. From human, tonsillar ILC were isolated and either expanded in culture or directly stimulated ex-vivo, as previously described38 (link). Human adult peripheral blood CD34+ cells were first enriched with CD34+ microbeads (Miltenyi Biotech), then purified to 99% by FACS. Cells were expanded in SCF (100 ng, Peprotech), IL-7 (100 ng, Peprotech), and IL-15 (50 ng, Peprotech) for 14 days. Tofacitinib (Sigma) or PF-06651600 (Pfizer) was added to cultured tonsillar cells at the indicated concentration 48-hours before stimulation, to cultured CD34+ cells for 72-hours before analysis, or ex-vivo at the time of stimulation. Human cells were stimulated for 7–8 hours and mouse cells for 5 hours before analyzing protein by intracellular stain or CBA of supernatants.
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