Doxorubicin was purchased from LC Laboratories (Woburn, MA). Rabbit polyclonal anticaspase-8 and anticaspase-3 antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Mouse monoclonal anti-β-actin, rabbit polyclonal anti-FADD, and rabbit polyclonal anti-Bax antibodies were obtained from Abcam (Cambridge, MA), Bioss Inc. (Woburn, MA), and Abgent Inc. (San Diego, CA), respectively. HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were purchased from Abcam.
Hrp conjugated anti rabbit and anti mouse secondary antibodies
Manufactured by Abcam
HRP-conjugated anti-rabbit and anti-mouse secondary antibodies are laboratory reagents used to detect the presence of rabbit or mouse primary antibodies in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry. These secondary antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric or chemiluminescent reaction, enabling the visualization and quantification of the target primary antibodies.
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2 protocols using hrp conjugated anti rabbit and anti mouse secondary antibodies
1
Doxorubicin-Induced Apoptosis Signaling
2
Immunoblot Analysis of Actin and TRPC6
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Mouse monoclonal anti-actin (Cat. No. ab3280) and rabbit polyclonal anti-TRPC6 (Cat. No. ab81111) primary antibodies and HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were purchased from Abcam (Cambridge, MA). DMSO, hyperforin, and tert-Butyl hydroperoxide were purchased from Sigma-Aldrich. BAPTA, FK506, VIVIT, Ac-DEVD-CHO, Fas blocking antibody, ionomycin, pluronic F-127, and Fura-2 AM were purchased from Assay Biotechnology (Sunnyvale, CA), Cell Signaling Technology (Danvers, MA), Cayman Chemical Company (Ann Arbor, MI), Biotium Inc. (Hayward, CA), ProSpec-Tany TechnoGene Ltd (Rehovot, Israel), Cayman Chemical Company, Anaspec (Fremont, CA), and Life Technologies, respectively.
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