In brief, after formalin fixing, cells were permeabilized with 0.1% Triton X-100 in TBS for 5 minutes, followed by a 30-minute block with goat serum. Next, cells were subjected to incubation with the AAK1 antibody (1:50, PA5408, Abmart) for 1h and hatched with FITC-conjugated goat anti-rabbit IgG (1:100, GB22303, servicebio) at room temperature for 2 hours at 37°C. Subsequently, the nucleus was marked with DAPI (BL105A, Biosharp). Finally, the cells were captured using a fluorescence microscope (Olympus, Tokyo, Japan).
Pa5408
Manufactured by Abmart
The PA5408 is a laboratory equipment product. It is designed to perform specific functions within a laboratory setting. The core function of this product is to provide a necessary tool for scientific research and analysis, though its exact intended use is not specified.
Automatically generated - may contain errors
Lab products found in correlation
Ps08936, by Abmart (2 mentions)
T55719, by Abmart (2 mentions)
Pa1748, by Abmart (2 mentions)
Ta5228, by Abmart (2 mentions)
Fluorescence microscope, by Olympus (1 mentions)
Fitc conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions)
Bl105a, by Biosharp (1 mentions)
Gb22303, by Wuhan Servicebio Technology (1 mentions)
Gb11001, by Wuhan Servicebio Technology (1 mentions)
Gb111141, by Wuhan Servicebio Technology (1 mentions)
3 protocols using pa5408
1
Immunofluorescent Localization of AAK1 Protein
2
Western Blotting Antibody Validation
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Western blotting assay was executed as previously reported 23 (link), with rabbit anti-human antibodies for AAK1 (1:2000, PA5408, Abmart), Notch3 (1:1000, PS08936, Abmart), GLS (1:2000, T55719, Abmart), MMP-2 (1:1000, PA1748, Abmart), MMP-9 (1:2000, TA5228, Abmart), and β-actin (1:2000, GB11001, Servicebio).
3
Immunohistochemical Analysis of Signaling Pathways
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The HE, IHC protocol and result analysis method were performed as previously described 24 (link), with rabbit anti-human antibodies for AAK1 (1:200, PA5408, Abmart), Notch3 (1:10, PS08936, Abmart), GLS (1:100, T55719, Abmart), MMP-2 (1:100, PA1748, Abmart), MMP-9 (1:300, TA5228, Abmart), and Ki-67 (1:200, GB111141, Servicebio).
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