Roziglitazone

Manufactured by Cayman Chemical

Roziglitazone is a synthetic compound used as a laboratory reagent. It functions as a selective agonist for the peroxisome proliferator-activated receptor gamma (PPAR-gamma), a nuclear receptor involved in the regulation of glucose and lipid metabolism.

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2 protocols using roziglitazone

Adipogenic Differentiation of Cells

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Freshly sorted cells were expanded as described above. After 5–7 days, medium was changed to adipogenic induction medium consisting of DMEM + 10% FBS + 1% penicillin/streptomycin + 1mM Dexamethasone (Sigma) + 100nM Insulin (Sigma) + 1mM Roziglitazone (Cayman Chemical) + 0.5 mM 3-isobutyl-1-methylxanthine (Sigma) for 2 days, and then replaced with adipogenic differentiation medium consisting of DMEM + 10% FBS + 1% penicillin/streptomycin + 100nM Insulin. Cells were kept in differentiation medium for 6 days, and then fixed with 4% Paraformaldehyde and stained with Oil Red O (Sigma) for one hour at room temperature [11 (link)]. Oil Red O staining of lipid droplets within adipocytes was analyzed by standard microscopy using an Olympus IX51 inverted microscope.

Hettmer S., Lin M.M., Tchessalova D., Tortorici S.J., Castiglioni A., Desai T., Mao J., McMahon A.P, & Wagers A.J. (2015). Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity. Experimental cell research, 340(1), 43-52.

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Modulation of GPR Signaling Pathways

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All agonists, drugs and inhibitors were dissolved in glycerol, DMSO or water. Sodium salt of SCFAs were from Sigma and used in a range of concentrations from 0.5 to 8 mM. GPRs agonists: GPR41: 4-CMTB (1 μM Tocris) and Tiglic acid (1–10 mM Sigma); GPR43: AR420626 (1 μM Cayman) and 1-MCPC (1mM Sigma); GPR109a: Niacine (1–10 mM, Sigma) and MK1903 (1 μM Tocris). GPRs sub-unit inhibitors used were: Pertussis toxin (Ptx 0.2 μg/ml) and U73122 (10 μM) from Sigma. HDAC inhibitors: Trichostatin A (TSA 1 μM Sigma), SAHA (5 μM Sigma) and valproic acid (VPA 5 mM Sigma). SP1 inhibitor Mithramycin A (0.1 μM Sigma). PPARγ activators: Pioglitazone (5 μM), Roziglitazone (10 μM) and PPARγ inhibitor G9662 (100 μM), from Cayman. NF-kB inhibitor BAY 11-7082 (40 μM). AP-1 inhibitor SR-11302 (10 μM Tocris). STAT3/Jak2 inhibitor Cucurbitacin I (1 μM) from Tocris. IFNγ (100 U/ml) and TNFα (10 ng/ml) were from Peprotech. Final concentration of DMSO had no detectable effect on cells viability or responses.

Martin-Gallausiaux C., Larraufie P., Jarry A., Béguet-Crespel F., Marinelli L., Ledue F., Reimann F., Blottière H.M, & Lapaque N. (2018). Butyrate Produced by Commensal Bacteria Down-Regulates Indolamine 2,3-Dioxygenase 1 (IDO-1) Expression via a Dual Mechanism in Human Intestinal Epithelial Cells. Frontiers in Immunology, 9, 2838.

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