Ska 31

Cyclopiazonic acid, L-NNA, carbachol (CCH), TEA, ACh, and INDO were purchased from Sigma. Ouabain was purchased from ApexBio. SKA-31, SKF96365, and GSK-7975A were purchased from MedChemExpress. SN-6 was purchased from Tocris. The most of the reagents were dissolved in DMSO at final concentration of less than 0.1%, which did not alter vascular activities in the experiments.

All salts were from Sangon Biotech in Shanghai. Acetylcholine, N^ω-nitro-L-arginine and indomethacin were offered by Sigma-Aldrich (St Louis, USA). NE was purchased from GRANDPHARMA (China) co. LTD. 17β-Estradiol, LPS, Propyl pyrazole triol (PPT), MPP hydrochloride (MPP), G-1, G-15, Diarylpropionitrile (DPN), PHTPP, ouabain, TRAM-34, apamin, U73122, U73343, 2-APB and SKA-31 were supplied by MedChemExpress (MCE; New Jersey, USA), SN-6 was provided by Tocris (Bristol, UK). LiCl was supplied by Macklin (China). The human phospholipase C Beta 1 Polyclonal antibody (Cat No. 26551-1-AP) and human ITPR1-specific Polyclonal antibody (Cat No. 19962-1-AP) were from proteintech. The commercial kits of serum ALT, AST, LD and BUN were from Neobioscience (Jiancheng Bioengineering Institute, Nanjing, China). The commercial kit of serum DAO was from Solarbio. The commercial kit of serum Cr was from Beijing Leagene Biotechnology co. LTD.