Anti vav2

Manufactured by Santa Cruz Biotechnology

Anti-Vav2 is a laboratory research reagent used to detect and study the Vav2 protein. Vav2 is a guanine nucleotide exchange factor involved in regulating the actin cytoskeleton and cell signaling pathways. Anti-Vav2 can be used in various techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify the Vav2 protein in biological samples.

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Lab products found in correlation

Anti ar n 20, by Santa Cruz Biotechnology (1 mentions) Anti histone, by Santa Cruz Biotechnology (1 mentions) Anti sod, by Santa Cruz Biotechnology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Blebbistatin, by Bio-Techne (1 mentions) Nocodazole, by Merck Group (1 mentions) Anti phosphotyrosine 4g10, by Merck Group (1 mentions) Anti eb1, by BD (1 mentions) Anti rhoa, by Cytoskeleton (1 mentions) Ephrina5 fc, by R&D Systems (1 mentions) Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions)

2 protocols using anti vav2

Western Blot Analysis of Cellular Proteins

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Cellular proteins were extracted and separated in 10-12% SDS-PAGE gels, and western blot analyses were performed as previously described (36 (link)). The antibodies used were: anti-AR (N-20; 1:1000; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), anti-AR-V7 (1:500; Precision Antibody), anti-Histone (1:1000; Santa Cruz), anti-SOD (1:1000; Santa Cruz), anti-Cleaved PARP (1:1000; Cell Signaling), anti-Vav3 (1:1000, Cell Signaling), anti-Vav2 (Santa Cruz), anti-actin (1:500; Santa Cruz), or anti-FLAG (1:1000, Sigma).
Densitometry was performed using ImageJ software (43 ).

Magani F., Peacock S.O., Rice M.A., Martinez M.J., Greene A.M., Magani P.S., Lyles R., Weitz J.R, & Burnstein K.L. (2017). Targeting AR Variant-coactivator Interactions to Exploit Prostate Cancer Vulnerabilities. Molecular cancer research : MCR, 15(11), 1469-1480.

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Cell Migration Assay with HGF Stimulation

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PC-3 cells were maintained as previously described (Astin et al., 2010 (link)). Cells were serum starved for 24 h and then treated with HGF (10 ng ml⁻¹) overnight prior to all assays to stimulate cell migration.
Ephrin-A5/Fc was obtained from R&D systems. HGF was obtained from Peprotech. Blebbistatin was from Tocris and Nocodazole was from Sigma (supplementary material Table S3). The following antibodies were used; anti-Glu tubulin (rabbit polyclonal, Chemicon), anti-Tyr tubulin (rat polyclonal, AbD Serotec), anti-EB1 (mouse monoclonal, BD Transduction laboratories), anti-RhoA (monoclonal mouse, Cytoskeleton), anti-Vav2 (rabbit polyclonal, Santa Cruz), anti-EphA2 (mouse monoclonal, Upstate), anti-cortactin was used as a primary antibody control for Vav2 immunoprecipitation (mouse monoclonal, Upstate), anti-phosphotyrosine 4G10 (mouse monoclonal, Millipore). EphA4 antibody was a kind gift from David Wilkinson (NIMR, UK). Antibody dilutions are given in supplementary material Table S2.

Batson J., Maccarthy-Morrogh L., Archer A., Tanton H, & Nobes C.D. (2014). EphA receptors regulate prostate cancer cell dissemination through Vav2–RhoA mediated cell–cell repulsion. Biology Open, 3(6), 453-462.

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