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21 protocols using ensite precision

1

Endocardial Geometry and Scar Mapping for Arrhythmia

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Endocardial geometry creation relied on impedance (EnSite Precision; Abbott Inc) and magnetic (MediGuide™ or EnSite Precision; Abbott Inc) points acquisition in sinus rhythm. In addition to geometry, voltage data were acquired, and electroanatomical mapping (EAM) was created by the EnSite Precision cardiac mapping system (EAM-endoLV). To improve accuracy, field scaling and respiratory compensation were applied. Bipolar electrograms were filtered at 30–300 Hz. Electrical identification of scar areas was done through bipolar EAM using a voltage amplitude–based criterion (with values <0.3 mV identifying “deep scar” and values >1.5 mV healthy myocardium). Fractionated signals identified either by the physician per-procedure using Jaïs and colleagues’ definition of local abnormal ventricular activation16 (link) or through EnSite Precision fractionation maps (threshold = 3) were used to locate scar areas and arrhythmogenic substrate. Voltage data from EAM was our gold standard in the clinical decision to determine the ablation locations.
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2

Visualization of Radiofrequency Wire for Transseptal Puncture

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Percutaneous femoral venous access was obtained using vascular ultrasound guidance. Two 8-Fr sheaths were placed in the right femoral vein; one 10-Fr long sheath and another 7-Fr sheath were placed in the left femoral vein. A 9-Fr ICE catheter (ViewFlex, Abbott, St. Paul, MN, USA) was inserted through the 10-Fr long sheath in the left femoral vein and advanced to the inferior vena cava (IVC) while maintaining an echo-free space at the tip of the transducer. The ICE catheter was then advanced toward the RA. A 6-Fr decapolar catheter (Inquiry Diagnostic Catheter, Abbott) was next inserted into the left femoral vein and into the IVC under EAM guidance (EnSite Precision, Abbott) to generate IVC, SVC, RA, and CS geometries. The 0.035-inch RF wire (VersaCross RF Wire, Baylis Medical, Montreal, QC, CA) was visualized on the EnSite system using “mapping mode” on the dedicated DuoMode extension cable (Baylis Medical) (Fig. 1).

Set up for visualization of 0.035 RF wire (VersaCross RF Wire, Baylis Medical Company, Montreal QC, CA). A DuoMode extension cable (Baylis Medical) was used to connect the RF wire to the EnSite velocity cardiac mapping system pin box (Abbott, St. Paul, MN) to enable visualization during superior vena cava (SVC) cannulation, drop-down to the fossa ovalis, and transseptal puncture

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3

Electrophysiology Study and Ablation Guidance

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Procedures were performed under general anesthesia in patients younger than 14 years, while local anesthesia and conscious sedation were used in older patients. Femoral vein access was obtained under ultrasound guidance. The 3D EAM system (EnSite NavX, Ensite Velocity, Ensite Precision, Abbott, St. Paul, MN, USA or Carto 3, Biosense Webster, Diamond Bar, CA, USA) was used for guidance of the catheters in the heart and advancement through the vasculature.
After femoral vein access was obtained, a 10-polar diagnostic catheter was advanced into the right atrium. The catheter was used to construct a partial 3D rendering of the right atrium and to mark the location of His potential. The catheter was then placed in the coronary sinus. Next, an additional 4- or 10-polar diagnostic catheter was inserted into the heart and placed on the basal section of the right side of the interventricular septum.
A standard electrophysiology study followed, with the aim of tachycardia induction. In cases of clear ventricular preexcitation, the induction of tachycardia was left to the physician’s discretion. If induction of tachycardia was not achieved or conduction over the accessory pathway (AP) was not detected, the protocol was repeated with an isoprenaline challenge. Standard diagnostic maneuvers were employed as needed to determine the type of induced tachycardia.
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4

Electroanatomical Mapping of Left Atrium

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We used EnSite Precision™ (Abbott Inc, St Paul, MN, USA), a non-fluoroscopic navigation system through which all electrodes in the heart are visualized within the electric field generated via six surface electrodes creating three orthogonal axes with the heart in their centre.33 (link) LA electroanatomical data were collected using the AFocus II™ spiral mapping catheter with 20 poles and a 4 mm spacing with a 20 mm loop diameter (Abbott Inc, St Paul, MN, USA). Complete LA maps were created prior to PVI in non-cardiac triggered mode, that enables the user to collect up to 8 s of electrogram data for every bipole. Recording was automatically started in stable catheter positions (< 10 mm catheter movement). Eight-second electrograms were collected for all 19 bipoles of the AFocus II. Points within 7 mm of the geometry shell were included in the map and the minimum interpolation distance for map colour was set at 7mm.
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5

High-Density Voltage Mapping of Atrial Fibrillation

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Left atrial (LA) geometry and voltage maps were created using the HD Grid catheter and EnSite Precision mapping system (Abbott) both in sinus rhythm and in atrial fibrillation (Figure 2). Myocardial voltages <0.07mV were considered to be scar and voltages >0.5mV were considered to be healthy active myocardium. Voltages between 0.07-0.5 were still considered to be low, but corresponding to viable tissue, as seen in many patients with diseased atrium.
The HDW algorithm selects the largest peak to peak voltage EGMs obtained from adjacent orthogonally orientated electrode pairs acquired simultaneously, automatically assigning the largest detected EGM as activation points to create high density voltage map. Sufficient points coverage of the entire LA was collected and in particular higher density points were taken around each PV ostium.
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6

Standardized Ablation Mapping Procedures

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Mapping procedure ablation sites included mainly left atrial but also right and biatrial mapping procedures. Despite the varying ablation sites, electroanatomic mapping procedures are highly standardized at our center. Access to the right ventricle is achieved by the right femoral vein and a 6F steerable decapolar catheter was placed in the coronary sinus (CS) as reference. If necessary, left atrial (LA) access was obtained by a double transseptal puncture equivalent to the cryoballoon PVI procedure. An 8.5F steerable sheath (Agilis NXT, Abbott Inc, St. Paul, MN) and an 8.5F non-steerable sheath (SL1, Abbott Inc, St. Paul, MN) were positioned in the left LA. A PV angiography is performed. For electroanatomic mapping approaches, ENSITE™ Precision (Abott®, Abbott Inc, St. Paul, MN) and CARTO® 3 System (Biosense Webster®, Irvine, California, USA) mapping systems are used. For PV evaluation, a 20-polar spiral mapping catheter (Advisor™ Abbott Inc, St. Paul, MN or Lasso®, Biosense Webster, Diamond Bar, CA, USA) was used, for high-resolution mapping a PentaRay® (Biosense Webster, Diamond Bar, CA, USA) or Advisor™ HD Grid (Abbott Inc, St. Paul, MN) were used. Additionally, an irrigated-tip ablation catheter with contact-force sensing was used (TactiCath™, Abbott Inc, St. Paul, MN; Thermocool Smarttouch® SF, Biosense Webster, Diamond Bar, CA, USA).
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7

High-Density Bipolar Voltage Mapping of Left Atrium

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Mapping was performed as previously reported. 4, 8 In brief, a HD-BVM map (LVZ <0.5 mV) was created simultaneously with LA surface reconstruction, guided by a three-dimensional electroanatomical mapping system (CARTO3, Biosense Webster, or Ensite Precision, Abbott) using a circular mapping catheter (Lasso, 4 mm interelectrode spacing, Biosense Webster, or Advisor FL, 3 mm interelectrode spacing, Abbott).
All mapping points were taken in sinus rhythm. For each mapping point, stable contact between the local atrial tissue and each pair of electrodes of the circular mapping catheter was required. Extra care was taken while collecting voltage points on the border of LVZ. 4 Sufficient quality of the acquired voltage points was verified by the following criteria 4
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8

Comprehensive Cardiac Imaging for Ablation

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Prior to the ablation procedure, all patients underwent transthoracic and transesophageal echocardiography to exclude possible LA thrombus formation. Additionally, individual left atrial anatomy was revealed using a 64‐slice CT scanner (Brilliance 64, Philips Medical Systems, Cleveland, OH, USA) with retrospective electrocardiography (ECG) gating and 3D reconstruction prior to the procedure. Scanning was performed at 120 kVp, with an effective tube current of 600 mAs. The slice collimation was 64 × 0.625 mm, with a gantry rotation time of 0.4 s and a pitch of 0.2. Images were reconstructed at 0.9 mm slice thickness at increments of 0.45 mm. Contrast enhancement with 80 ml of contrast agent (Imeron 400 MCT, Iomeprol 81.65 g/100 ml, Bracco, Konstanz, Germany) was injected at a flow rate of 5 ml/s and followed by a 50‐ml saline flush. A blinded observer (JP) analyzed, segmented, and measured each CCTA image visually and quantitatively with regard to the defined parameters and the morphological classification of the LAA using EnSite Precision™ (Abbott Medical GmbH, Eschborn, Germany). Multiplan volume‐rendered post‐processing was used to acquire a 3D perspective. After anatomical segmentation into PV, LA, and LAA, all 2‐dimensional (2D) and 3D measurements were conducted.
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9

Catheter Stability in Atrial Fibrillation Ablation

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This retrospective observational study included consecutively admitted patients who underwent initial ablation for AF using the EnSite Precision™ mapping system (Abbott, St. Paul, MN) at the Aichi Medical University Hospital between January 1, 2019 and December 31, 2019. Patients were excluded if they had previously undergone ablation treatment, insufficient three-dimensional coordinate data, or a common left PV. The patients were divided into two groups (GA and CS groups), and the ablation catheter stability during PVI was compared based on the distance traveled per second by the catheter distal tip. Anesthesia was administered under GA or CS at the preference of the patient and at the discretion of the attending physician. In addition, the clinical characteristics obtained from reviews of medical charts, periprocedural characteristics, and complications were evaluated.
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10

Ablation Procedures for Persistent Atrial Fibrillation

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Mapping and ablation procedures were guided using Ensite Precision (Abbott). A long steerable introducer (Agilis NxT Steerable, 82 cm; Abbott) was used to properly perform the transeptal puncture and electroanatomical mapping of the LA. A PentaRay catheter (20 poles, Biosense Webster) was connected to the pin box of the electrophysiology recording system using a custom-made adaptor. Then, the catheter was positioned sequentially at multiple locations of the endocardium to reconstruct the RA, coronary sinus (CS), and LA anatomy. Eight-second unipolar signals were acquired at each location. Data from the first 4 pigs with PersAF were used to develop a computational tool for in vivo procedures (development group). Twelve more PersAF pigs (ablation group) also underwent a subsequent second biatrial map to assess spatiotemporal stability of drivers. Finally, data were exported for intraprocedural signal processing.
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