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Ab118929

Manufactured by Abcam
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Sourced in United Kingdom, United States

Ab118929 is a recombinant protein product developed by Abcam. It is a research-use only product intended for laboratory applications.

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Lab products found in correlation

Ab203573, by Abcam (1 mentions) Ponceau, by Merck Group (1 mentions) Ab18258, by Abcam (1 mentions) Ab55160, by Abcam (1 mentions) Ab53036, by Abcam (1 mentions) Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions) Phospho ser133creb, by Cell Signaling Technology (1 mentions) Imagequant las 4000 mini system, by GE Healthcare (1 mentions) Ab8227, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Nb100 125, by Novus Biologicals (1 mentions) Enhanced bca protein assay kit, by Beyotime (1 mentions)

4 protocols using ab118929

1

Western Blot Protein Analysis Protocol

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Western blots were performed according to the protocol described previously [48 (link)]. The following antibodies were used: BDNF (abcam, ab203573, Cambridge, UK), ARC (abcam, ab118929, Cambridge, UK) and PSD-95 (abcam, ab18258, Cambridge, UK). Ponceau (Sigma-Aldrich, St. Louis, MO, USA) staining served as an internal loading control (Figure S1).
Hladik D., Buratovic S., Von Toerne C., Azimzadeh O., Subedi P., Philipp J., Winkler S., Feuchtinger A., Samson E., Hauck S.M., Stenerlöw B., Eriksson P., Atkinson M.J, & Tapio S. (2019). Combined Treatment with Low-Dose Ionizing Radiation and Ketamine Induces Adverse Changes in CA1 Neuronal Structure in Male Murine Hippocampi. International Journal of Molecular Sciences, 20(23), 6103.
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2

Westerns for Hippocampal Protein Analysis

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Protein was extracted from the hippocampi in rats with etomidate treatment or vehicle according to a previously described procedure.[14 (link)] Membranes were probed with primary antibodies against CREB, phospho-Ser133-CREB (#9104 and #9191, respectively, Cell Signaling, USA), Arc, c-fos, and Egr1 (ab118929, ab53036, and ab55160, respectively, Abcam, USA). Horseradish peroxidase conjugated anti-rabbit or anti-mouse secondary antibodies were used and visualized using the enhanced chemiluminescence kit (Thermo Scientific, USA). The density of each band was quantified using the ImageJ software (National Institutes of Health, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, ab1603, Abcam) was used as a loading control.
Li X., Lu F., Li W., Xu J., Sun X.J., Qin L.Z., Zhang Q.L., Yao Y., Yu Q.K, & Liang X.L. (2016). Underlying Mechanisms of Memory Deficits Induced by Etomidate Anesthesia in Aged Rat Model: Critical Role of Immediate Early Genes. Chinese Medical Journal, 129(1), 48-53.
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3

Hippocampal Protein Extraction and Analysis

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Proteins were extracted as described previously4 . Briefly, hippocampus was homogenized and dissolved in ice-cold lysis buffer (PBS, pH 7.4) containing a cocktail of protein phosphatase and protease inhibitors (21 μg/ml aprotinin, 0.5 μg/ml leupetin, 4.9 mM MgCl2, 1mM sodium-Meta-vanandante, 1% Triton X-100 and 1 mM PMSF) to avoid de-phosphorylation and degradation of proteins. All samples were centrifuged at 14000 × g at 4 °C for 7 min. The supernatant was then assayed for total protein concentration. Proteins were separated in 8.5% SDS-PAGE gel, transferred to PVDF membrane, blocked with 5% non-fat dry milk, followed by incubation with primary antibodies overnight at 4 °C. Then membranes were washed for three times, incubated with secondary antibody. The blots were visualized using ImageQuant LAS 4000 mini system (GE Healthcare). The antibodies of β-actin and Arc were purchased from Abcam (β-actin, 1:2000, ab8227; Arc, 1:1000, ab118929). All results were normalized against β-actin. The analysis was performed using Image J software.
Liu Z.H., Ding J.J., Yang Q.Q., Song H.Z., Chen X.T., Xu Y., Xiao G.R, & Wang H.L. (2016). Early developmental bisphenol-A exposure sex-independently impairs spatial memory by remodeling hippocampal dendritic architecture and synaptic transmission in rats. Scientific Reports, 6, 32492.
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4

Quantitative Western Blot Analysis of Synaptic Proteins

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Total protein from mPFC, extracted using RIPA lysis buffer and quantified by Enhanced BCA Protein Assay Kit (Beyotime P0010S), was separated by SDS-PAGE and transferred to PVDF membrane. After being blocked in TBS buffer containing 0.05% Tween-20 and 5% skim milk, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-Arc (Abcam; ab118929; 1:1000), anti-PSD95 (Abcam, ab18258, 1:1000), anti-spinophilin (Upstate 06–852, 1:1000), anti-TrkB (Sigma-Aldrich, 07–225, 1:1000), anti-mTOR (Abcam, ab2972, 1:1000), anti-Akt (Cell Signaling, #9272, 1:1000) and anti-Per2 (Novus biologicals NB100–125; 1:1000), After incubation with the corresponding secondary antibody, the immunoreactive signals were visualized by LI-COR ODYSSEY Fc Western Blotting Detection System ( and quantitated using Image Studio Ver5.2. The levels of these proteins in the stress offspring versus non-stress offspring were normalized by β-actin protein levels. In order to estimate background caused by non-specific binding of secondary antibody, a secondary control without the primary antibody was performed.
Dong E., Guidotti A., Zhang H, & Pandey S.C. (2018). Prenatal Stress Leads to Chromatin and Synaptic Remodeling and Excessive Alcohol Intake Comorbid with Anxiety-like Behaviors in Adult Offspring. Neuropharmacology, 140, 76-85.
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