Mrps16

Manufactured by Proteintech

MRPS16 is a protein that is a component of the mitochondrial ribosome. Mitochondrial ribosomes are responsible for synthesizing proteins necessary for the function of mitochondria, the organelles that generate energy in eukaryotic cells.

Automatically generated - may contain errors

Lab products found in correlation

Irdye 800cw goat anti rabbit igg, by LI COR (2 mentions) Irdye 680lt goat anti mouse igg, by LI COR (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Mrps35, by Proteintech (1 mentions) Coxii, by Abcam (1 mentions)

2 protocols using mrps16

Immunoblot Analysis of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific proteins were detected using rabbit polyclonal antibodies against: MRPL44 (16394-1-AP), MRPL23 (11706-1-AP), TACO1 (21147-1-AP), MRPS35 (16457-1-AP), MRPS16 (16735-1-AP) (Proteintech, diluted 1:1,000), MRPS34 (Sigma HPA042112, diluted 1:1,000), and mouse monoclonal antibodies against: β-actin (8226), porin (14734), NDUFA9 (14713), complex II (14715), complex III (14745), COXI (14705), COXII (198286), COXIV (14744) and complex V subunit a (14748; Abcam, diluted 1:1,000), in Odyssey Blocking Buffer (Li-Cor). IR Dye 800CW Goat Anti-Rabbit IgG or IRDye 680LT Goat Anti-Mouse IgG (Li-Cor) secondary antibodies were used and the immunoblots were visualized using an Odyssey Infrared Imaging System (Li-Cor). Examples of uncropped blots are shown in the Supplementary Fig. 8. Tissue-specific analysis was performed using a Proteintech mouse tissue blot (Cat. No M10005).

Richman T.R., Spåhr H., Ermer J.A., Davies S.M., Viola H.M., Bates K.A., Papadimitriou J., Hool L.C., Rodger J., Larsson N.G., Rackham O, & Filipovska A. (2016). Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Nature Communications, 7, 11884.

+ Open protocol

+ Expand

Comprehensive Protein Detection Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific proteins were detected using rabbit polyclonal antibodies against: MRPL44, MRPL37, MRPL23, MRPS12, MRPS16 (Proteintech, diluted 1:1000), MRPS34 (Sigma, diluted 1:1000), GAPDH, phosphorylated and non‐phosphorylated S6, phosphorylated and non‐phosphorylated Akt, phosphorylated and non‐phosphorylated 4EBP (Cell Signalling Technology, diluted 1:500) and mouse monoclonal antibodies against: phosphorylated and non‐phosphorylated AMPKα (Cell Signalling Technology, diluted 1:500), COXII (Abcam, diluted 1:1000), phosphorylated and non‐phosphorylated YAP, AFG3L2, LC3A/B, Glut2, Glut4, and Total OXPHOS Rodent WB Antibody Cocktail (Abcam, Diluted 1:1000) in Odyssey Blocking Buffer (Li‐Cor). IR Dye 800CW Goat Anti‐Rabbit IgG or IRDye 680LT Goat Anti‐Mouse IgG (Li‐Cor) secondary antibodies were used and the immunoblots were visualized using an Odyssey Infrared Imaging System (Li‐Cor).

Richman T.R., Ermer J.A., Siira S.J., Kuznetsova I., Brosnan C.A., Rossetti G., Baker J., Perks K.L., Cserne Szappanos H., Viola H.M., Gray N., Larance M., Hool L.C., Zuryn S., Rackham O, & Filipovska A. (2021). Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. Aging Cell, 20(7), e13408.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!