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Rhodamine b

Manufactured by Sinopharm
Sourced in China

Rhodamine B is a fluorescent dye that can be used as a labeling agent for various biological and analytical applications. It has the chemical formula C₂₈H₃₁ClN₂O₃ and is known for its intense pink-red color and strong fluorescent properties when exposed to specific wavelengths of light. Rhodamine B is commonly used in fluorescence microscopy, flow cytometry, and other analytical techniques where visualization and tracking of labeled samples are required.

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29 protocols using rhodamine b

1

Chitosan-Based Nanoparticle Formulation

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CUR, CS (Mw = 179.17 kDa, deacetylation degree ≥95%, viscosity = 100–200 mPa·s), NAC and Ellman’s reagent (DTNB, 5,50-dithiobis (2-nitrobenzoic acid)) were all obtained from Aladdin (Shanghai, China). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and 1-Hydroxybenzotrizole (HOBT) were purchased from Medpep Co., Ltd. (Shanghai, China). DMF was obtained from Shanghai Chemical Co., Ltd. (Shanghai, China). GMS was obtained from TianJin Bodi Chemical Holding Co., Ltd. (Tianjin, China). Miglyol 812 N (medium chain triglyceride, MCT)) was obtained from Sasol (Witten, Germany). Solutol HS15 (polyoxyethylene esters of 12-hydroxystearic acid) was supplied by BASF (Lud-wigshafen, Germany). Gelucire 44/14 was kindly gifted by Gattefosse (Paris, France). Rhodamine B was purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Purified water was used after deionization and filtration. All other chemicals and reagents used were of analytical grade or better.
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2

Nanocarrier Synthesis and Characterization

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PLGA 5050 1.5A was supplied
by Boehringer Ingelheim Pharma (Ingelheim am Rhein, Germany). NH2–PEG–NH2 [molecular weight (MW) 5000
Da] was obtained from Yare Biotechnology (Shanghai, China). FA and
MTT were obtained from Yuanye Biotechnology (Shanghai, China). Polyvinyl
alcohol (87–89% hydrolyzed, MW 13 000–23 000
Da) and JC-1 were purchased from Sigma-Aldrich (St Louis, MO, USA).
DCFH-DA, a fluorescent dye, was obtained from Nanjing Jiancheng Bioengineering
Institute (Nanjing, China). 4′,6-Diamidino-2-phenylindole (DAPI)
was obtained from Beyotime Institute of Biotechnology (Haimen, China).
Rhodamine B was obtained from Sinopharm Chemical Reagent (Shanghai,
China). HeLa, Hep G2, and HEK-293T cell lines were purchased from
American Type Culture Collection (ATCC). Acetonitrile was purchased
from Thermo Fisher Scientific.
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3

Synthesis and Characterization of Functionalized CNTs

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Raw CNTs (97 wt%) were purchased from Shenzhen Nanotech Port Co., Ltd. (Shenzhen, China). Melamine, Rhodamine B (RhB, 98 wt%), methyl orange (MO, 98 wt%), methyl blue (MB, 98 wt%), chlortetracycline hydrochloride (CTC, 98 wt%), sodium chloride (NaCl, 99.5 wt%), sodium bicarbonate (NaHCO3, 99.5 wt%), sodium nitrate (NaNO3, 99 wt%), ethanol (C2H5OH, 99.7 wt%), sulfuric acid (H2SO4, 95–98 wt%), nitric acid (HNO3, 65–68 wt%), sodium sulfate (Na2SO4, 99 wt%), hydrochloric acid (HCl, 36–38 wt%), sodium hydroxide (NaOH, 96 wt%) and p-benzoquinone (pBZQ, 97 wt%) were purchased form Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Humic acid (HA, 90 wt%), tetracycline (TC, 98 wt%), potassium peroxydisulfate (PDS, 98 wt%), furfuryl alcohol (FFA,98 wt%), 4-hydroxy-TEMPO (TEMPOL, 98 wt%), 5,5-dimethyl-pyridine N-oxide (DMPO, 97 wt%) and 4-amino-2,2,6,6-tetramethylpiperidine (TEMP, 98 wt%) were provided by Aladdin Reagent Co., Ltd. (Shanghai, China). All chemicals were of analytical grade and used without any purification in the experiments.
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4

Synthesis of Gold Nanoparticles with Tailored Properties

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Gold(III) chloride trihydrate (HAuCl4·3H2O), acetonitrile (C2H3N, HPLC grade), sodium hydroxide (NaOH), hydrochloric acid (HCl), tetraethyl orthosilicate (C8H20O4Si, TEOS), isopropanol (C3H8O), ethanol (C2H6O), ammonia (NH3·H2O), p-methoxy nitrobenzene (C7H7NO3), rhodamine B (C28H31ClN2O3, RhB); ammonium formate (CH5NO2), potassium persulfate (K2S2O8), tert-butyl alcohol (C4H10O), p-benzoquinone (C6H4O2) were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). l-glutathione reduced (C10H17N3O6S, GSH), hexadecylamine (C16H35N, HDA), branched poly-ethylenimine (Mw ~25,000, BPEI) and titanium isopropoxide (C12H28O4Ti, TIP), 5,5-dimethyl-1-pyrroline-N-oxide (C6H11NO, DMPO) were obtained from Sigma-Aldrich. All materials were analytical grade and used as received without further purification. Deionized (DI) water used in the synthesis was from local sources.
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5

Synthesis and Characterization of Bismuth-Based Nanomaterials

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Bismuth nitrate (Bi(NO3)3·5H2O), potassium bromide (KBr), ethylene glycol (EG), natural flake graphite, sodium nitrate (NaNO3), iron(iii) chloride hexahydrate (FeCl3·6H2O), ethylenediamine (EDA), sodium acetate trihydrate (CH3COONa·3H2O), hydrogen peroxide (H2O2), absolute ethyl ethanol, 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), rhodamine B (RhB), methyl blue (MB), methyl orange (MO), acid red (AR), amino black (AB) were purchased from Sinopharm Chemical Reagent Co. Ltd., China. Other reagents used in this study, including concentrated sulfuric acid (H2SO4), potassium permanganate (KMnO4) and concentrate hydrochloric acid (HCl) were obtained from Xinyang Chemical Reagent Co. Ltd., China. All reagents were of analytical grade and used without further purification.
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6

Photocatalytic Degradation of Organic Pollutants

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Ferric nitrate (Fe(NO3)3·9H2O), sodium chloride (NaCl), sodium carbonate (Na2CO3) hydrochloric acid (HCl), potassium persulfate (K2O8S2), sodium hydroxide (NaOH), isopropanol (IPA, C3H8O), methanol (MeOH, CH3OH), ethanol (C2H6O), p-benzoquinone (BQ, C6H4O2), kaolin (Al2O3·2SiO2·2H2O), and ciprofloxacin (CIP, C17H19ClFN3O3) of analytical grade, were purchased from Sinopharm Chemical Reagent Co. Ltd. Rhodamine B (RhB, C28H31ClN2O3), methyl orange (MO, C14H14N3NaO3S), and methylene blue (MB, C16H18N3S·Cl) were obtained from the market. In all the experiments, all solutions were prepared with deionized water.
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7

Dex-loaded PEI/HA Nanoparticles for Inflammatory Arthritis

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Dex was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Egg PC was purchased from Seebio Biotech (Shanghai) Co., Ltd. (Shanghai, China). Sodium hyaluronate (HA, Mw < 10 K) was purchased from Lifecore Biomedical Co., Ltd (Chaska, MN, USA). PEI (Mw 25 kDa, branched), polyvinyl alcohol (PVA), dichloromethane, and acetone were purchased from Sigma–Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and RAW 264.7 cells were obtained from Wuhan Procell Biological Technology Co., Ltd (Wuhan, China). 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was obtained from Beyotime Institute of Biotechnology (Shanghai, China). Rhodamine B was obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Complete Freund’s adjuvant (CFA) was obtained from Chondrex (Redmond, WA, USA). Rat plasma tumor necrosis factor alpha (TNF-α) ELISA kits and interleukin (IL)-6 ELISA kits were obtained from Elabscience Biotechnology Co., Ltd (Wuhan, China). All other chemical reagents and solvents used were of analytical grade.
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8

Synthesis of Graphene-based Composites

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The reagents used in this paper include Zinc acetate dihydrate, sodium hydroxide (NaOH), and Rhodamine B (RhB), which are all analytically pure and purchased from Sinopharm Chemical Reagent Co. Ltd., MCNT and GO are purchased from Suzhou Tanfeng Graphene Technology Co. Ltd., as shown in Table 1.
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9

Photocatalytic Dye Degradation Synthesis

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All chemicals were obtained from commercial suppliers at analytical grade and used without further purification. Fe(NO3)3∙9H2O, urea, ethanol (EtOH), powdered active carbon (PAC), and 30% H2O2 solution were obtained from Sinopharm Chemical Reagent Co., Ltd. Rhodamine B, tertiary butanol (TEA), L‐histidine (L‐His), p‐benzoquinone (p‐BQ), 5,5‐dimethyl‐1‐pyrroline N‐oxide (DMPO), and 2,2,6,6‐tetramethylpiperidine (TEMP) were obtained from Shanghai Aladdin Bio‐Chem Technology Co., Ltd. Methylene blue (MB) and crystal violet (CV) were obtained from Adamas‐Beta (Shanghai) Chemical Reagents Co., Ltd.
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10

Synthesis and Characterization of Magnetic Nanoparticles

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The chemical and reagents were of analytical grade and used as such without any further purification. All the chemicals viz. sodium tungstate (Na2WO4·2H2O), ferric acetylacetonate (C15H21FeO6), methylbenzene (C6H5CH3), rhodamine B (C28H31C1N2O3), oleic acid (C18H34O2), hydrazine hydrate (N2H4·H2O), hydrochloric acid (HCl), and absolute ethanol (C2H5OH) were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the solutions were prepared in deionized water obtained from an ultra-filtration system.
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