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Brdu apc staining kit

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The BrdU-APC staining kit is a laboratory tool designed to detect and quantify the incorporation of bromodeoxyuridine (BrdU) into DNA. BrdU is a thymidine analog that is incorporated into the DNA of proliferating cells during the S phase of the cell cycle. The kit utilizes an allophycocyanin (APC)-conjugated anti-BrdU antibody to label the incorporated BrdU, allowing for the identification and analysis of proliferating cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Cytofix cytoperm, by BD (2 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Cd11b, by BioLegend (1 mentions) Collagenase dispase, by Roche (1 mentions)

Spelling variants of this product

BrdU staining kit (20 citations) APC BrdU kit (17 citations)

5 protocols using brdu apc staining kit

1

BrdU Labeling and Intracellular Staining

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For in vitro BrdU labeling, BrdU was added to culture media to a final concentration of 10 μm. 3 h later, cells were collected and stained first with surface marker (see above), followed by BrdU staining using a BrdU-APC staining kit (BD Biosciences). For Ki67 and caspase3 staining, BM cells were first stained with surface markers to define the progenitor population, followed by fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and intracellular staining with Ki67-PE or Caspase3-PE.
Zhao J., Xiu Y., Fu L., Dong Q., Borcherding N., Wang Y., Li Q., De Silva N.S., Klein U., Boyce B.F, & Zhao C. (2021). TIFAB accelerates MLL-AF9−Induced acute myeloid leukemia through upregulation of HOXA9. iScience, 24(12), 103425.
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2

In Vivo BrdU Labeling and Ki67 Staining of Bone Marrow

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For in vivo BrdU labeling, 5-day-old matched control or caNIK mice were injected intraperitoneally with BrdU (10 mg/ml) to a final concentration of 50 ug/g body weight. One hour later, mice were sacrificed and BM cells were stained first with surface marker, followed by BrdU staining using a BrdU-APC staining kit (BD Biosciences). For Ki67 staining, BM cells were first stained with surface markers to define the progenitor population, followed by fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and intracellular staining with Ki67-PE.
Xiu Y., Xue W.Y., Lambertz A., Leidinger M., Gibson-Corley K, & Zhao C. (2016). Constitutive activation of NIK impairs the self-renewal of hematopoietic stem/progenitor cells and induces bone marrow failure. Stem cells (Dayton, Ohio), 35(3), 777-786.
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3

Skin and Lymph Node Tissue Dissociation

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Ears were treated for 30 m at 37°C with 1μg of activated Collagenase Dispase (Roche) and deactivated using EDTA. Single cell suspensions of skin and LN were stained with Abs from eBioscience unless otherwise stated: LiveDead Aqua (Lifetech), CD45 (BD clone 30-F11), CD4 (BD clone GK1.5), Thy1.1 (clone H1S51), Thy1.2 (BD clone 53-2.1), CD25 (clone PC61.5), CD127(clone A7R34), FcER1 (clone MAR-1), T1/ST2 (MBBiosystems clone DJ8), CD49b (clone DX5), Gr-1 (Biolegend clone RB6-8C5), CD11b (Biolegend clone M1/70) and Foxp3 (clone FJK-16S) using Foxp3 fix/perm reagents. For 5-bromo-2-deoxyuridine (BrdU), mice received 1mg of BrdU i.p. in PBS 4 hours prior to harvest. Cells were stained with BrdU APC Staining kit (BD).
Billroth-MacLurg A.C., Ford J., Rosenberg A., Miller J, & Fowell D.J. (2016). Regulatory T cell numbers in inflamed skin are controlled by local inflammatory cues that upregulate CD25 and facilitate antigen-driven local proliferation. Journal of immunology (Baltimore, Md. : 1950), 197(6), 2208-2218.
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4

In Vivo BrdU Proliferation Assay

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For analysis of cellular proliferation in vivo, mice were administered 2 mg BrdU suspended in 500 µl PBS at 16 h (anti-viral kinetic experiment) or every day for 5 d (steady state proliferation in mixed BM chimeras) prior to analysis. Incorporated BrdU was detected using the APC BrdU staining kit (BD Biosciences).
Roychoudhuri R., Clever D., Li P., Wakabayashi Y., Quinn K.M., Klebanoff C.A., Ji Y., Sukumar M., Eil R.L., Yu Z., Spolski R., Palmer D.C., Pan J.H., Patel S.J., Macallan D.C., Fabozzi G., Shih H.Y., Kanno Y., Muto A., Zhu J., Gattinoni L., O’Shea J.J., Okkenhaug K., Igarashi K., Leonard W.J, & Restifo N.P. (2016). BACH2 regulates CD8+ T cell differentiation by controlling access of AP-1 factors to enhancers. Nature immunology, 17(7), 851-860.
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5

In Vivo BrdU Proliferation Assay

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For analysis of cellular proliferation in vivo, mice were administered 2 mg BrdU suspended in 500 µl PBS at 16 h (anti-viral kinetic experiment) or every day for 5 d (steady state proliferation in mixed BM chimeras) prior to analysis. Incorporated BrdU was detected using the APC BrdU staining kit (BD Biosciences).
Roychoudhuri R., Clever D., Li P., Wakabayashi Y., Quinn K.M., Klebanoff C.A., Ji Y., Sukumar M., Eil R.L., Yu Z., Spolski R., Palmer D.C., Pan J.H., Patel S.J., Macallan D.C., Fabozzi G., Shih H.Y., Kanno Y., Muto A., Zhu J., Gattinoni L., O’Shea J.J., Okkenhaug K., Igarashi K., Leonard W.J, & Restifo N.P. (2016). BACH2 regulates CD8+ T cell differentiation by controlling access of AP-1 factors to enhancers. Nature immunology, 17(7), 851-860.
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