Susceptibility testing for all bacteria was performed using the microdilution broth method as outlined by the Clinical and Laboratory Standards Institute (CLSI) (46 ). Mueller Hinton Broth 2 (MH, Sigma-Aldrich, 90922) was used. Briefly, 2 μL of 50X compound stocks were added to the wells of a 96-well round well plate. Live and dead controls received 2 μL of vehicle. 88 μL of MH broth was added to all wells except the dead control wells, which received 98 μL of MH broth. 100 μL of an overnight culture of bacteria was then added to 10 mL of MH broth and grown until the culture reached a turbidity equal to 1 X 107 – 2 x 108 cfu/mL (based on a previously determined calibration curve). The culture was then diluted to 5 X 106 cfu/mL and 10 μL of this solution was added to each well except the dead controls for a final of 5 X 105 cfu/mL. Plates were incubated at 37 °C for 16-20 h. Absorbance was then read on a Tecan infinite M200 Pro plate reader at λ = 600 nm. Minimum Inhibitory Concentration (MIC) values were defined as the lowest concentration of compound that resulted in ≥ 90% growth inhibition.
Mueller hinton broth 2 mh
Manufactured by Merck Group
Mueller Hinton Broth 2 (MH) is a microbiological growth medium used for the cultivation and susceptibility testing of bacteria. It is a commonly used medium in clinical microbiology laboratories. The broth provides the necessary nutrients and growth factors to support the growth of a wide range of bacteria.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mueller hinton broth 2 mh
1
Microdilution Broth Assay for Bacterial Susceptibility
2
Antibacterial Activity Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibacterial activity
analysis for all bacteria was performed using the microdilution broth
method as outlined by the Clinical and Laboratory Standards Institute
(CLSI).47 Mueller Hinton Broth 2 (MH, Sigma-Aldrich,
90922) was used for all testing. Testing was performed as previously
described.58 (link) Turbidity (OD600) of the wells
was determined using a SpectraMax iD3 platereader (Molecular Devices).
For the compounds that hit during initial screens, minimum inhibitory
concentrations were determined. A minimum of three biological replicates
were performed. Ciprofloxacin was used as a control in these assays.
Colistin was also used as a control in the colistin resistant strains.
analysis for all bacteria was performed using the microdilution broth
method as outlined by the Clinical and Laboratory Standards Institute
(CLSI).47 Mueller Hinton Broth 2 (MH, Sigma-Aldrich,
90922) was used for all testing. Testing was performed as previously
described.58 (link) Turbidity (OD600) of the wells
was determined using a SpectraMax iD3 platereader (Molecular Devices).
For the compounds that hit during initial screens, minimum inhibitory
concentrations were determined. A minimum of three biological replicates
were performed. Ciprofloxacin was used as a control in these assays.
Colistin was also used as a control in the colistin resistant strains.
3
Microdilution Broth Assay for Bacterial Susceptibility
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!