Naïve B cells were MACS-purified by negative selection from splenocytes of WT, Dnase1l3−/−, Ifnar1−/− or Dnase1l3−/−Ifnar1−/− mice using CD43 microbeads. Purified B cells were labelled with 3 μM Cell trace CFSE (Invitrogen), in PBS+ 2% BSA for 10 mins at RT. CFSE labelled cells were washed twice with PBS and subsequently suspended in RPMI + 10% FBS + 2mM L-glutamine + 10μM β-Mercaptoethanol and plated in round bottom 96 well plates at a concentration of 0.5×106 cells/ well in 200μl media. Cells were either left unactivated or activated with: (1) 5 μg/ml anti-IgM (Jackson Immunoresearch) + anti-CD40 (LEAF-purified, BioLegend) and 5 ng/ml recombinant mouse IL-4 (R&D systems) OR (2) 1μg/ml LPS (LPS-EK Ultrapure, InvivoGen) + 5 ng/ml recombinant mouse IL-4. Unactivated (UA) and activated (A) B cells were supplemented or not with recombinant mouse IFNα1 (BioLegend) at indicated concentrations. B cells were analyzed for activation, proliferation and differentiation after 3 days of culture.
Recombinant mouse ifnα1
Manufactured by BioLegend
Recombinant mouse IFNα1 is a laboratory reagent that is a type I interferon produced in a recombinant expression system. It is a cytokine that plays a role in the immune response.
Automatically generated - may contain errors
2 protocols using recombinant mouse ifnα1
1
Modulation of B Cell Activation by IFNα
2
Intranasal Recombinant IFNα1 in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant mouse IFNα1 (Biolegend, 751806) was administered intranasally. Control mice were similarly administered vehicle solution of 0.1% bovine serum album (BSA) in PBS. Doses (8 × 104 U, 10 µL/nostril) administered at time of infection were suspended in the inoculum under brief isoflurane anesthesia. Subsequent doses (8 × 104 U, 5 µL/nostril) were administered at 1–3 h post-infection (hpi), as indicated, under brief isoflurane anesthesia.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!