Pd123319

Mice were randomly divided into 4 groups: i) Control (normal saline) (n = 10), ii) C21 (n = 6), iii) PD (n = 5) and iv) PD + C21 (n = 5). AT2R antagonist PD123319 (16099, Cayman chemicals) was administered (5 mg/kg, i.p.) 15 min prior to a single dose of C21 (0.3 mg/kg, i. p.). All mice were euthanized 2-h after C21 treatment.

Acetylcholine (Ach), phenylephrine (Phe), Indomethacin (Indo), and angiotensin II (Ang II) were bought from Sigma-Aldrich (St. Louis, MO, United States). 9,11-dideoxy-9α,11α-methanoepoxy Prosta-glandin F2α (U46619), L-NAME, nifedipine, 1H-[1,2,4]-oxadiazolo-[4,3-alpha]-quinoxalin-1-one (ODQ) and Losartan, Enalapril, PD123319 were from Cayman Chemical (MI, United States). Fura-2 was supplied by Beyotime Biotechnology (Shanghai, China). THP hydrochloride (purity by HPLC ≥98.0%) was bought from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). THP was dissolved in dimethyl sulfoxide (DMSO) while other drugs were prepared in distilled water.

Captopril, Olmesartan, CGP42112A, and alloxan monohydrate were purchased from Sigma Chemical Co. (Saint Louis, MO, USA). PD123319 from Cayman Chemical Co. (Ann Arbor, MI, USA). Ethanol, methanol, and xylene from Merck (Rio de Janeiro, RJ, Brazil). Sodium heparin and sterile saline solution from Roche (São Paulo, SP, Brazil). All solutions were prepared immediately before use.