Blood samples were collected for fasting insulin, fasting glucose, 2-h PGPG, fasting lipid profile, liver function test and thyroid function test. Serum adiponectin, hsCRP, LH, FSH, total testosterone, DHEAS and serum AMH were also done. The hormonal evaluation was done on the 2nd or 3rd days of the menstrual cycle or de novo in the case of amenorrhoeic females. Serum insulin, LH, FSH, total testosterone and DHEAS assay were measured using the automated electrochemiluminescence immunoassay method (Cobas e 411 analyser, Roche Diagnostics International Ltd). hs-CRP was measured by a particle-enhanced immunoturbidimetric test (AU480 Chemistry Analyser, Beckman Coulter). AMH was measured using Serolisa Human AMH ELISA Kit (AU480 Chemistry Analyser, Beckman Coulter). Adiponectin was measured using BioVendor Human Adiponectin Kit (competitive ELISA, RD195023100).
Au480 chemistry analyser
Manufactured by Beckman Coulter
Sourced in United States
The AU480 Chemistry Analyser is an automated clinical chemistry analyser designed for use in medical laboratories. It is capable of performing a variety of clinical chemistry tests on biological samples such as blood, urine, and other body fluids. The AU480 is used to measure the levels of various analytes, enzymes, and other substances in the samples, providing data that can be used for clinical diagnosis and patient monitoring.
Automatically generated - may contain errors
Lab products found in correlation
Cobas e411 analyser, by Roche (1 mentions)
Vacutainer tube, by BD (1 mentions)
Accu chek, by Roche Diabetes Care (1 mentions)
Mek 7222k, by Nihon Kohden (1 mentions)
Xe 2100, by Sysmex (1 mentions)
Quantichrom creatinine assay kit, by BioAssay Systems (1 mentions)
Unicel dxc 800 synchron clinical system, by Beckman Coulter (1 mentions)
11 protocols using au480 chemistry analyser
1
Hormonal Evaluation of Reproductive Health
2
Blood Analysis Protocol for Cattle Grazing
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To assess the steers’ nutritional and health status, blood samples were collected at the start and end of the grazing period from the sample 50 steers by caudal venipuncture into 10 mL heparin-containing BD Vacutainer tubes. Plasma was isolated using a portable horizontal bench-top centrifuge (StatSpin Express 4, Iris Sample Processing, Westwood, MA, USA) at 4000× g for 5 min at room temperature. Plasma samples were transferred into labelled 15 mL aliquot tubes and stored at −20 °C pending laboratory analysis. Plasma non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), total bilirubin, creatinine and glucose were analysed using the colorimetric, 3-hydroxybutyrate dehydrogenase, modified diazo, kinetic modified Jaffe and hexokinase methods of the AU480 chemistry analyser (Beckman Coulter, Inc., Brea, CA, USA), respectively, according to the manufacturer’s procedures.
3
Enzymatic CK and IFCC LDH Assay
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Creatine kinase (CK) activity was determined by the Enzymatic method (Hexokinase) and lactate dehydrogenase (LDH) was determined by the IFCC (International Federation of Clinical Chemistry) standard method using AU480 Chemistry Analyser (Beckman coulter). Both assays were calculated after correction for total protein.
4
Fasting Lipid Profile Measurements
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Participants fasted the night before their venous blood was drawn. Using the Beckman AU480 chemistry analyser, fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured.
5
Biochemical Assessment of Oral Toxicity
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At the end of the oral toxicity assays, the biochemical parameters of liver function tests (total protein, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP)) and kidney function tests (blood urea nitrogen (BUN) and creatinine) were determined by standard techniques in an AU480 chemistry analyser (Beckman Coulter, Brea, CA, USA).
6
Post-Surgical Blood and Metabolic Analysis
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On day 17 after surgery, tail tip blood and whole blood from the ophthalmic vein were collected from the mice before euthanasia; serum was also obtained after blood coagulation. Blood glucose level was measured using a blood glucose meter (ACCU-CHEK; Roche Diabetes Care GmbH, Mannheim, Germany) after starving the mice for 12 h. All blood routine tests, including the determination of white blood cell count (WBC), haemoglobin (Hb) level, red blood cell count (RBC), haematocrit (HCT), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) level, mean corpuscular volume (MCV), red blood cell distribution width coefficient of variation (RDW-CV), platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and platelet haematocrit (PCT), were performed using an automatic blood cell analyser (MEK-7222K; NIHON KOHDEN CORP., Japan). Additionally, biochemical parameters, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), blood urea nitrogen (BUN), and serum creatinine (Scr), were measured using an automatic biochemical analyser (AU480 Chemistry Analyser; Beckman Coulter, Brea, CA, USA).
7
Measurement of Intraerythrocytic Magnesium Levels
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Regarding intra-erythrocyte magnesium measurements, 5 ml blood in one heparinized tube and one EDTA tube was collected from each patient after a fasting period of at least 8 h. 100μL of whole blood were added to 2 ml of 2% ascorbic acid in pure water in order to obtain lysed erythrocyte cells. Heparinized tube was centrifuged in order to separate plasma. Magnesium levels in the present lysed cells and in previously obtained plasma were measured on Beckman Coulter AU480 Chemistry Analyser (Brea, CA). EDTA tube was used to measure Haematocrit in automated haematology analyser XE-2100 (Sysmex). Results were calculated as follows: intra-erythrocytes Mg = [lysed cells Mg*21 (dilution factor)*Haematocrit (HCT)]/100 and expressed as mg/dL RBC (Red Blood Cells). Reference values: 1,98–3,21 mmol/L (4,56–7,8 mg/dL) as described in [8 (link)].
8
Fasting Blood Sample Analysis Protocol
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Fasting blood samples were drawn in the morning (from 0500 h) after a minimum 8 h fast by a trained nurse who also provided HIV screening using a rapid diagnostic test according to standard operating procedures. The samples were transported, on ice, to the onsite project laboratory for processing (mean time between collection and processing 2.6 hours). Samples for glucose measurement were collected in sodium fluoride (NaF) tubes. We used the hexokinase glucose-6-phosphate dehydrogenase method (Beckman Coulter AU480 Chemistry Analyser, Johannesburg, South Africa), with sufficient sensitivity (range 0.555–44.4 mol/L) to determine glucose concentration in the study samples. Serum total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were measured using enzymatic assays on the Beckman Coulter Chemistry Analyser. The same protocol was used to take these measures at follow-up.
9
Comprehensive Metabolic Profiling Protocol
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All biochemical analyses were performed using an AU480 Chemistry Analyser (Beckman Coulter, Mt Waverly, VIC, Australia) according to manufacturers' instructions. Total (Hb) and HbA1c were measured in whole blood. Circulating glucose, triglyceride and cholesterol (total, HDL- and LDL-cholesterol), creatinine, albumin and CRP were measured in plasma. Glucose, microalbumin and creatinine concentrations were measured in urine. Total PAI-1, a marker of subclinical chronic inflammation whose expression is predictive of insulin resistance and development of T2D (Festa et al., 2006 (link)), was measured in plasma using a commercially available ELISA kit (Abcam, Melbourne, VIC, Australia) according to the manufacturer's instructions.
10
Serum and Urinary Biomarker Analysis
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Serum creatinine and blood urea nitrogen levels were determined by an automatic chemistry analyser (AU480 Chemistry Analyser, Beckman Coulter, Atlanta, Georgia). Urinary creatinine level was determined by use of a QuantiChrom creatinine assay kit (DICT‐500, Bioassay Systems, Hayward, CA), according to manufacturers' protocol. Urinary albumin was standardized to urine creatinine and expressed as mg/mg Ucr.
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