Rabbit anti ck19

Manufactured by Abcam

Sourced in United States

Rabbit anti-CK19 is a primary antibody that recognizes the cytokeratin 19 (CK19) protein. CK19 is a member of the keratin family of intermediate filament proteins, which are essential structural components of epithelial cells. This antibody can be used to detect and identify CK19-expressing cells in various research applications.

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Anti-CK19 (27 citations) Rabbit anti- (5 citations)

6 protocols using rabbit anti ck19

Quantification of Pancreatic ADM and AFLs

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For quantification of ADM and AFLs, random whole pancreatic sections stained with hematoxylin and eosin H&E were analyzed. For immunohistochemical staining, sections of paraffin embedded pancreata were rehydrated and antigen retrieval was performed using Antigen Unmasking Solution (Vector Laboratories). Overnight incubation with the following primary antibodies was done at 4°C: goat anti-CPA1 (R&D), rabbit anti-CK19 (Abcam), rabbit anti-GFP (Santa Cruz Biotechnology), mouse anti-Ki67 (BD), rabbit anti-P53 (Vector Laboratories), goat anti-Amylase (Santa Cruz Biotechnology), rabbit anti-p44/p42 MAPK (Cell Signaling) and FITC-conjugated DBA-lectin (Vector Laboratories). Biotin-conjugated secondary antibodies were incubated for 1 h at room temperature, following development with ABC and DAB kits (both Vector Laboratories). Nuclear counterstaining was performed using haematoxylin. For immunofluorescence, sections were incubated with fluorophore-conjugated secondary antibody for 1 h at room temperature. Slides were mounted with DAPI hardset antifade mounting medium.

von Figura G., Fahrenkrog-Petersen L., Hidalgo-Sastre A., Hartmann D., Hüser N., Schmid R.M., Hebrok M., Roy N, & Esposito I. (2017). Atypical flat lesions derive from pancreatic acinar cells. Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 17(3), 350-353.

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Immunohistochemical Profiling of Cell Markers

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Immunohistochemistry was performed as previously described^{65 (link)}. The following primary antibodies were used: rabbit anti-Cleaved caspase 3 (1:200; Cell Signalling #9661), mouse anti-CD45 (1:20, BDPharmigen #550539), rabbit anti-CK19 (1:1000; Abcam #ab133496), goat anti-CPA1 (1:300, RD Systems #AF2765), mouse anti–Ki-67 (1:400; BDPharmigen #550609), mouse anti-MUC5AC (1:200; Cell Marque #292M-95), rabbit anti-p65 (C-20) (1:200, Santa Cruz #sc-372), rabbit anti-phospho-STAT3 (Y705) (1:100, Cell Signaling #9145).

Hidalgo-Sastre A., Lubeseder-Martellato C., Engleitner T., Steiger K., Zhong S., Desztics J., Öllinger R., Rad R., Schmid R.M., Hermeking H., Siveke J.T, & von Figura G. (2020). Mir34a constrains pancreatic carcinogenesis. Scientific Reports, 10, 9654.

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Immunohistochemical Analysis of TLR2 in Liver

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The tissues were sliced with the thickness of 5 μm sections with routine deparaffinization. The sections were incubated with rabbit-anti-TLR2 antibody, rabbit-anti-α-SMA, rabbit-anti-CK-19 (Abcam, Cambridge, USA), Goat-anti-rabbit IgG H&L (HRP) (Affinity bioscience, Jiangsu, China) and DAB staining (Solarbio, Beijing, China). Details of the antibodies were presented in Table 1. The expression levels of TLR2 in liver were digitized and analyzed using Image-Pro Plus software.

Wang Y., Zhang X., Wang X., Zhang N., Yu Y., Gong P., Zhang X., Ma Y., Li X, & Li J. (2023). Clonorchis sinensis aggravates biliary fibrosis through promoting IL-6 production via toll-like receptor 2-mediated AKT and p38 signal pathways. PLOS Neglected Tropical Diseases, 17(1), e0011062.

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Detecting Breast and Epithelial Markers

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Western blot and Immunofluorescence staining were performed to detect breast and epithelial cell markers. Primary antibodies used were mouse anti-EMA (Dako), rabbit anti-Pan-Ck (Abcam), rabbit anti-Ck19 (Abcam), mouse anti-estrogen receptor (ER) α (Abcam), rabbit anti-ERβ (Abcam) and mouse anti-progesterone receptor (PR) (Abcam). Secondary antibodies used in Western blot analysis were horse-radish peroxidase conjugated goat anti-rabbit and anti-mouse antibodies (Dako). Secondary antibodies used in immunofluorescence study were Alexa Fluor 594 goat anti-rabbit (Life Technologies) and Alexa Fluor 488 goat anti-mouse (Life Technologies). Antibody concentrations used in Western blot and immunofluorescence staining were as recommended by the antibody manufacturers.

Yong J.W., Choong M.L., Wang S., Wang Y., Lim S.Q, & Lee M.A. (2014). Characterization of ductal carcinoma in situ cell lines established from breast tumor of a Singapore Chinese patient. Cancer Cell International, 14, 94.

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Protein Expression Profiling in ATII Cells after MC-LR Exposure

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Proteins were purified from lungs or ATII cells after exposure to MC-LR for 48 h. Western blot analysis of cellular lysates was performed as previously described (Wang et al., 2014 (link)). The following primary antibodies were employed: Rabbit anti-CK18, rabbit anti-CK19, rabbit anti-SP-C, rabbit anti-E-cadherin, rabbit anti-ZO-1, rabbit anti-occludin, rabbit anti-vimentin, rabbit anti-ERK1/2, rabbit anti-p-ERK1/2, rabbit anti-Akt, rabbit anti-p-Akt, mouse anti-MEK1/2, rabbit anti-p-MEK1/2, rabbit anti-PTEN, rabbit anti-GAPDH, mouse anti-MEK1/2 and mouse anti-β-actin (Abcam Inc. Cambridge, MA). Horseradish peroxidase-conjugated goat anti-rabbit/mouse IgG (Boster, Wuhan, China) was used as the secondary antibody. Immunoreactive protein bands were detected using an Odyssey Scanning System (LI-COR Inc).

Wang C., Gu S., Yin X., Yuan M., Xiang Z., Li Z., Cao H., Meng X., Hu K, & Han X. (2016). The toxic effects of microcystin-LR on mouse lungs and alveolar type II epithelial cells. Toxicon : official journal of the International Society on Toxinology, 115, 81-88.

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Histopathological Analysis of Rat Liver Tissues

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Liver tissues were collected immediately at the time rats were sacrificed. Liver samples were fixed by paraformaldehyde and embedded in paraffin. Sections were subjected to hematoxylin and eosin (H&E), immunohistochemistry (IHC), Sirius red staining according to the standard protocols. Antibodies used for IHC staining are as follows: Rabbit anti-Fah (AbboMax, San Jose, CA, 1:3000), Rabbit anti-Ki67 (novocastra, 1:1000), Rabbit anti-α-SMA (Abcam, 1:200), Rabbit anti-Ck19 (Abcam, 1:200).

Zhang L., Shao Y., Li L., Tian F., Cen J., Chen X., Hu D., Zhou Y., Xie W., Zheng Y., Ji Y., Liu M., Li D, & Hui L. (2016). Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I. Scientific Reports, 6, 31460.

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