SDS-PAGE and Western blotting were performed by standard techniques. The blots were developed with nitroblue tetrazolium chloride/5-bromo-4-chloro-3′-indolyl phosphate (Amresco, Solon, OH) or Pierce ECL Western blotting substrate (Thermo Scientific), using rabbit polyclonal anti-rPspA serum as primary antibodies and mouse anti-rabbit IgG alkaline phosphatase conjugate (Sigma-Aldrich) as secondary antibodies.
Mouse anti rabbit igg alkaline phosphatase conjugate
Manufactured by Merck Group
Mouse anti-rabbit IgG alkaline phosphatase conjugate is a laboratory reagent used in various immunoassay techniques. It consists of mouse-derived antibodies specific to rabbit immunoglobulin G (IgG) that are chemically conjugated to the enzyme alkaline phosphatase. This conjugate serves as a detection tool, binding to rabbit IgG and enabling the visualization of target analytes through the enzymatic activity of alkaline phosphatase.
Automatically generated - may contain errors
Lab products found in correlation
Nitro blue tetrazolium chloride 5 bromo 4 chloro 3 indolyl phosphate, by Merck Group (3 mentions)
Anti his antibody, by Merck Group (2 mentions)
5 bromo 4 chloro 3 indolyl phosphate, by Avantor (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg alkaline phosphatase conjugate, by Merck Group (1 mentions)
4 protocols using mouse anti rabbit igg alkaline phosphatase conjugate
1
Protein Detection via SDS-PAGE and Western Blot
2
SDS-PAGE and Western Blotting Techniques
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SDS-PAGE and Western blotting procedures were done by standard techniques. Blots were developed with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (Sigma–Aldrich) as a substrate, using previously obtained rabbit polyclonal anti-CjaA, anti-CjaD, anti-rCjaAD (Pawelec et al., 2000 (link); Łaniewski et al., 2012 (link)), or anti-LysM (this work) sera as primary antibodies, and mouse anti-rabbit IgG alkaline phosphatase conjugate (Sigma–Aldrich) as secondary antibodies.
3
Western Blot Immunodetection Protocol
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SDS-PAGE and Western blotting procedures were done by standard techniques. Blots were developed with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (Sigma–Aldrich) as a substrate, using previously obtained rabbit polyclonal anti-CjaA, anti-CjaD (Pawelec et al., 2000 (link); Łaniewski et al., 2012 (link)), or anti-rCjaAD (this work) sera or anti-His antibodies (Sigma–Aldrich) as primary antibodies, and mouse anti-rabbit IgG alkaline phosphatase conjugate (Sigma–Aldrich) or goat anti-mouse IgG alkaline phosphatase conjugate as secondary antibodies.
4
SDS-PAGE and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SDS-PAGE and Western blotting procedures were done using standard techniques. Blots were developed with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich) as a substrate using previously obtained rabbit polyclonal anti-rCjaA or anti-rCjaD serum [Łaniewski et al., 2012; Pawelec et , 2000] or anti-His antibodies (Sigma-Aldrich) as primary antibodies and mouse anti-rabbit IgG alkaline phosphatase conjugate (Sigma-Aldrich) or goat anti-mouse IgG alkaline phosphatase conjugate as secondary antibodies.
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