Ciprofloxacin hydrochloride

Manufactured by Merck Group

Sourced in United States, Germany

Ciprofloxacin hydrochloride is a synthetic antibacterial agent belonging to the fluoroquinolone class. It is a crystalline powder used as a raw material in the production of various pharmaceutical products.

Automatically generated - may contain errors

Lab products found in correlation

Gentamicin sulfate, by Merck Group (3 mentions) Vancomycin hydrochloride, by Merck Group (3 mentions) Sodium hydroxide (naoh), by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Sodium chloride (nacl), by Merck Group (3 mentions) Protease xiv, by Merck Group (2 mentions) Horseradish peroxidase hrp, by Merck Group (2 mentions) Tetracycline hydrochloride, by Merck Group (2 mentions) Acetic acid ch3cooh, by Merck Group (2 mentions) Curcumin, by Merck Group (2 mentions) Chitosan, by Merck Group (2 mentions) Milli q water, by Merck Group (2 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions) 2 2 diphenyl 1 picrylhydrazyl radical dpph, by Merck Group (1 mentions) Mueller hinton broth (mhb), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by Sartorius (1 mentions) Trypsin ethylenediaminetetraacetic acid edta solution, by Harvard Bioscience (1 mentions) Folin ciocalteu s phenol reagent, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions)

33 protocols using ciprofloxacin hydrochloride

Preparation of Ciprofloxacin Stock Solution

Check if the same lab product or an alternative is used in the 5 most similar protocols

1000 μg/ml ciprofloxacin hydrochloride USP standard stock solution was prepared by dissolving 10 mg ciprofloxacin hydrochloride (Sigma–Aldrich, St. Louis, Missouri, USA) in 10 ml of tris-buffered saline solution (TBS) which consisted of TrisHCl 100 mM (Sigma–Aldrich) and NaCl 150 mM (Sigma–Aldrich) adjusted pH to 7.6 by using NaOH and HCl.

Chotitumnavee J., Parakaw T., Srisatjaluk R.L., Pruksaniyom C., Pisitpipattana S., Thanathipanont C., Amarasingh T., Tiankhum N., Chimchawee N, & Ruangsawasdi N. (2018). In vitro evaluation of local antibiotic delivery via fibrin hydrogel. Journal of Dental Sciences, 14(1), 7-14.

+ Open protocol

+ Expand

Silk Nanomaterial Compression and Bioactive Incorporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amorphous silk nanomaterials (ASN) was packed into predesigned mold, followed by hot pressing at 632 MPa and variable temperatures (25 °C, 65 °C, 95 °C, 125 °C, 145 °C, 175 °C) for 15 min. After hot pressing, the samples were cooled down to room temperature and used for characterizations. To incorporate bioactive molecules in the system, the silk powder was doped with ciprofloxacin hydrochloride (5 wt%, Sigma-Aldrich), gentamicin sulfate (5 wt%, Sigma-Aldrich), horseradish peroxidase (HRP) (1 wt%, Sigma-Aldrich), and protease XIV (1 wt%, Sigma-Aldrich) by thorough mixing and subject to hot processing.

Guo C., Li C., Vu H.V., Hanna P., Lechtig A., Qiu Y., Mu X., Ling S., Nazarian A., Lin S, & Kaplan D.L. (2019). Thermoplastic Molding of Regenerated Silk. Nature materials, 19(1), 102-108.

+ Open protocol

+ Expand

Evaluating Antioxidant and Antibacterial Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ciprofloxacin hydrochloride, Folin–Ciocalteu’s phenol reagent, gallic acid, sodium carbonate, formic acid (HPLC), acetonitrile (HPLC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), (R)-(+)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), silver nitrate (AgNO₃), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Steinheim, Germany). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was obtained from Merck (Darmstadt, Germany). Dulbecco’s Modified Eagle’s Medium/Dulbecco’s Minimum Essential Media (DMEM) and penicillin-streptomycin solution were purchased from Biological Industries Israel Beit Haemek Ltd. (Beit Haemek, Israel). Mueller-Hinton broth was supplied by Oxoid (Basingstoke, UK). Fetal bovine serum and trypsin/ethylenediaminetetraacetic acid (EDTA) solution were provided by Biochrom (Berlin, Germany). All other chemicals and reagents were of analytical grade. Ultra-pure water was obtained from SG Water Ultra Clear TWF water purification system (Barsbüttel, Germany).

Macovei I., Luca S.V., Skalicka-Woźniak K., Horhogea C.E., Rimbu C.M., Sacarescu L., Vochita G., Gherghel D., Ivanescu B.L., Panainte A.D., Nechita C., Corciova A, & Miron A. (2023). Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects. Antioxidants, 12(4), 797.

+ Open protocol

+ Expand

Antimicrobial Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cefazolin was obtained from Acros Organics. Ciprofloxacin hydrochloride, linezolid and vancomycin hydrochloride were obtained from Sigma-Aldrich (St. Louis, MO, USA). Tetracycline hydrochloride was obtained from Alfa Aesar. Sorangicin A was provided by HZI, Braunschweig. Stock solutions were prepared in Milli-Q water or dimethyl sulfoxide (DMSO, Sigma-Aldrich). Minimum inhibitory concentrations (MICs) were determined in cation-adjusted Mueller-Hinton broth (MHB2) using the broth microdilution method as recommended by the Clinical and Laboratory Standards Institute (CLSI). The MIC was defined as the lowest concentration of the antibiotic causing complete inhibition of visible growth of the microorganism (CLSI, 2017) [58 ].

Fries F., Kany A.M., Rasheed S., Hirsch A.K., Müller R, & Herrmann J. (2023). Impact of Drug Administration Routes on the In Vivo Efficacy of the Natural Product Sorangicin a Using a Staphylococcus aureus Infection Model in Zebrafish Embryos. International Journal of Molecular Sciences, 24(16), 12791.

+ Open protocol

+ Expand

Antimicrobial and Antifungal Susceptibility Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antimicrobial stock solutions of vancomycin hydrochloride (Sigma, MO), clindamycin hydrochloride (RPI Corp., IL), ciprofloxacin hydrochloride (Sigma-Aldrich, WY), tobramycin sulfate (Sigma, MO), methicillin sodium salt (Sigma, MO), and linezolid (AK Scientific, CA) were diluted in phosphate-buffered saline to a maximum concentration of 100 μg/ml. The stock solutions of the antifungal drugs amphotericin B and fluconazole were diluted in PBS to maximum concentrations of 16 μg/ml and 2 mg/ml, respectively. Any subsequent dilutions needed for the antimicrobial susceptibility assays were made in PBS. To study the effect of drugs in preventing biofilm formation, 50 nl of drugs was printed at 2-fold dilutions on top of the hydrogel spots after initial printing of the cells. The microarray(s) containing drugs was then incubated in a humidified hybridization cassette for a maximum of 24 h, gently washed in PBS, stained with FUN-1, and analyzed using the microarray scanner.

Srinivasan A., Torres N.S., Leung K.P., Lopez-Ribot J.L, & Ramasubramanian A.K. (2017). nBioChip, a Lab-on-a-Chip Platform of Mono- and Polymicrobial Biofilms for High-Throughput Downstream Applications. mSphere, 2(3), e00247-17.

+ Open protocol

+ Expand

Silk Nanomaterial Compression and Bioactive Incorporation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Guo C., Li C., Vu H.V., Hanna P., Lechtig A., Qiu Y., Mu X., Ling S., Nazarian A., Lin S, & Kaplan D.L. (2019). Thermoplastic Molding of Regenerated Silk. Nature materials, 19(1), 102-108.

+ Open protocol

+ Expand

Phage PEV20 Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phage PEV20 was provided by AmpliPhi Biosciences AU at a titre of 10¹⁰ plaque forming unit (PFU)/mL in phosphate buffered saline (PBS, 0.01 M phosphate buffer, 0.0027 M KCl and 0.137 M NaCl) ^{[28 ]}. This phage was formerly isolated from the sewage treatment plant in Olympia (WA, USA) by Kutter lab (Evergreen phage lab). Ciprofloxacin hydrochloride was purchased from Sigma-Aldrich Inc.

Lin Y., Chang R.Y., Britton W.J., Morales S., Kutter E., Li J, & Chan H.K. (2019). Inhalable combination powder formulations of phage and ciprofloxacin for P. aeruginosa respiratory infections. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 142, 543-552.

+ Open protocol

+ Expand

Antibiotic and Phytochemical Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oxacillin sodium monohydrate, erythromycin (base), gentamicin sulfate, ciprofloxacin hydrochloride, tetracycline hydrochloride and clindamycin phosphate were purchased from Sigma-Aldrich (Steinheim, Germany). Morusin and kuwanon G (purity min. 97%) were obtained from Carbosynth Limited (Berkshire, UK).

Aelenei P., Rimbu C.M., Horhogea C.E., Lobiuc A., Neagu A.N., Dunca S.I., Motrescu I., Dimitriu G., Aprotosoaie A.C, & Miron A. (2020). Prenylated phenolics as promising candidates for combination antibacterial therapy: Morusin and kuwanon G. Saudi Pharmaceutical Journal : SPJ, 28(10), 1172-1181.

+ Open protocol

+ Expand

Ciprofloxacin Kidney Exposure Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

ciprofloxacin hydrochloride (Sigma-Aldrich), which is water soluble, was diluted in the SMCM at final concentrations of 100 and 200 μg/mL; these concentrations reflect the approximate ciprofloxacin concentrations to which the kidney is exposed and are in accordance with other study protocols of ciprofloxacin treatment for HASMCs (12 (link), 21 (link), 37 (link), 47 (link)). In all experiments, the prepared 100 and 200 μg/mL ciprofloxacin solutions were treated for 24 hours, along with the start of the rhythmic strain.

Xiang B., Abudupataer M., Liu G., Zhou X., Liu D., Zhu S., Ming Y., Yin X., Yan S., Sun Y., Lai H., Wang C., Li J, & Zhu K. (2023). Ciprofloxacin exacerbates dysfunction of smooth muscle cells in a microphysiological model of thoracic aortic aneurysm. JCI Insight, 8(2), e161729.

+ Open protocol

+ Expand

Antibiotic Minimum Inhibitory Concentration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ciprofloxacin hydrochloride, amikacin, aztreonam, colistin sulphate and tobramycin sulphate were purchased from Sigma-Aldrich Inc. The Minimum inhibitory concentration (MIC) of antibiotics was determined as per ^{[25 (link)]} with minor modifications. Briefly, 100 µL of antibiotics (512, 256, 128, 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125 mg/L) were added to 100 µL of early-log phase bacterial culture (10⁶ CFU/mL). The treated culture was incubated overnight at 37 °C with continuous shaking at 150 rpm. MIC was determined to be the concentration of antibiotic at which the optical density (OD₆₀₀) was equal to that of a cell-free blank control.

Lin Y., Kyung Chang R.Y., Britton W.J., Morales S., Kutter E, & Chan H.K. (2018). Synergy of nebulized phage PEV20 and ciprofloxacin combination against Pseudomonas aeruginosa. International journal of pharmaceutics, 551(1-2), 158-165.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!