The tissue culture polystyrene (TCPS) surface was coated with specifically composed biomimetic matrix according to the protocol of Prasad Chennazy and Krishnan.13 (link) One milliliter of the composed matrix comprised 5 mg in-house isolated human cryoprecipitate (clottable fibrinogen and fibronectin), 0.2% gelatin (Sigma), and 100 μg hyaluronic acid (in-house purified and characterized14 (link)), 20 μg of released platelet growth factor (PGF) prepared per Resmi et al.,15 (link) 25 μg laminin V (Sigma), and 250 ng recombinant epidermal growth factor (EGF; Sigma). Briefly, matrix composite was clotted on thrombin adsorbed TCPS to get a thin fibrin layer, and dishes were lyophilized under sterile atmosphere using a freeze drier (Edwards, Modulyo 4K). The TCPS immobilized with composite fibrin matrix were then stored at 4°C to 6°C until use.
Recombinant epidermal growth factor egf
Manufactured by Merck Group
Recombinant epidermal growth factor (EGF) is a laboratory product produced by Merck Group. EGF is a protein that stimulates cell growth and division. It plays a role in the regulation of cell proliferation, differentiation, and survival.
Automatically generated - may contain errors
Lab products found in correlation
Laminin 5, by Merck Group (1 mentions)
Modulyo 4k, by Edwards Lifesciences (1 mentions)
Gelatin, by Merck Group (1 mentions)
Lapatinib, by Chemietek (1 mentions)
Afatinib bibw2992, by Selleck Chemicals (1 mentions)
Sp600125, by Selleck Chemicals (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
2 protocols using recombinant epidermal growth factor egf
1
Biomimetic Matrix Coating for Cell Culture
2
Kinase Inhibitors and Signaling Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lapatinib was purchased from ChemieTek (Indianapolis, IN), afatinib (BIBW 2992) was from Selleckchem (Houston, TX), and canertinib (CI-1033, PD-183805) was kindly provided by Pfizer (Groton, CT), respectively. All kinase blockers were dissolved in DMSO and diluted 1:1,000 or 1:2,000 in medium before use. Recombinant epidermal growth factor (EGF) and heregulin-b1 (HRG-b1) were purchased from Sigma (St. Louis, MO). Fetal calf serum (FCS), DMEM, a-MEM, and RPMI 1640 were from GIBCO (Karlsruhe, Germany). The JNK inhibitor SP600125 and the STAT5 inhibitor II (IQDMA) were obtained from Selleckchem and from Merck (Darmstadt, Germany), respectively.
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