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Progesterone receptor

Manufactured by Cell Signaling Technology
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Progesterone receptor is a laboratory product that functions as a protein receptor for the steroid hormone progesterone. It is used in research applications to study the role of progesterone signaling in various biological processes.

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Lab products found in correlation

Hybond c membrane, by GE Healthcare (1 mentions) Androgen receptor, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Hybond, by Cytiva (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Eif4g, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Cyclin d3, by Cell Signaling Technology (1 mentions) Eif4e, by Cell Signaling Technology (1 mentions) P 4e bp1 t37 46, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Prb s780, by Cell Signaling Technology (1 mentions) Anti puromycin, by Cell Signaling Technology (1 mentions) Tff1 ps2, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Gata3, by Cell Signaling Technology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Progesterone receptor A/B (clone D8Q2J) (3 citations) Progesterone receptor A/B (3 citations)

2 protocols using progesterone receptor

1

Protein Expression Analysis in Cells

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Cells were lysed using Merck phosphosafe buffer (Calbiochem) and protein concentration was quantified using a Pierce protein assay (ThermoScientific) as per manufacturer's instructions. Samples were denatured at 100°C for 10 minutes before separation of protein in the cytosolic extracts according to size using SDS-PAGE gel electrophoresis. Proteins were transferred electrophoretically from the gels onto a nitrocellulose membrane (Hybond C Membrane (GE Healthcare). Antibodies used for detection of protein were: Oestogen receptor a 1:1000 (Santa Cruz 8005), Progesterone receptor 1:1000 (Cell signalling), Androgen receptor 1:1000 (Santa Cruz 7305), HER-2 1:1000 (Santa Cruz 33684), HER-3 1:1000 (Santa Cruz 285), MDM2 3:1000 (Calbiochem), p53 2:1000 (Vector), p21 1:100 (Calbiochem), GAPDH 1:3000 (Santa Cruz) and Actin 1:1000 (Sigma).
McCormick A., Earp E., Elliot K., Cuthbert G., O'Donnell R., Wilson B.T., Sutton R., Leeson C., Thomas H.D., Blair H., Fordham S., Lunec J., Allan J, & Edmondson R.J. (2017). Functional characterisation of a novel ovarian cancer cell line, NUOC-1. Oncotarget, 8(16), 26832-26844.
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2

Protein Expression Profile Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-puromycin (Kerafast EQ0001), pAKT S473 (Cell Signaling Technology (CST) 4060), p4EBP1 T37/46 (CST 2855), Cyclin D1 (CST 55506), Estrogen Receptor Alpha N-terminus (CST 13258), Estrogen Receptor Alpha C-terminus (CST 8644), Progesterone Receptor (CST 8757), EIF4G (CST 2498), EIF4E (CST 9742), 4EBP1 (CST 9644), Beta Actin (CST 4967), Myc (CST 18583), GATA3 (CST 5852), Cyclin D3 (CST 2936), CDK4 (CST 12790), pRb S780 (CST 9307), E2F1 (CST 3742), GREB1 (CST 65171), TFF1/pS2 (CST 15571), IGFBP4 (CST 31025), cleaved PARP (CST 5625). Secondary Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP (Thermo Fisher 65–6120).
Boyer J.A., Sharma M., Dorso M.A., Mai N., Amor C., Reiter J.M., Kannan R., Gadal S., Xu J., Miele M., Li Z., Chen X., Chang Q., Pareja F., Worland S., Warner D., Sperry S., Chiang G.G., Thompson P.A., Yang G., Ouerfelli O., de Stanchina E., Wendel H.G., Rosen E.Y., Chandarlapaty S, & Rosen N. (2024). eIF4A controls translation of estrogen receptor alpha and is a therapeutic target in advanced breast cancer. bioRxiv.
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