Reporter cell lines were created by transducing Jurkat (E6.1) T cells with Cignal Lenti NFAT reporter (Catalogue number CLS-015 L), Cignal Lenti NFκB reporter (Catalogue number CLS-013L), or Cignal Lenti Negative Control (CLS-NCL) lentiviral particles purchased from Qiagen. An equal volume of Bright-Glo™ Luciferase Assay System (Promega) was added to an equal volume of cells. After 5 min, the cells were lysed, and the lysate was transferred to a black Costar EIA/RIA polystyrene half area 96-well plate (Corning). Luminescence was measured with the VICTOR™ X4 Multi-Plate Reader (PerkinElmer).
Cignal lenti nfat reporter
Manufactured by Qiagen
The Cignal Lenti NFAT reporter is a lentiviral reporter system designed to monitor the activity of the Nuclear Factor of Activated T-cells (NFAT) transcription factor in mammalian cells. It consists of an NFAT-responsive firefly luciferase construct that can be delivered into target cells using lentiviral transduction.
Automatically generated - may contain errors
2 protocols using cignal lenti nfat reporter
1
Transduced Jurkat Cells Reporter Assay
2
NFAT, NFkB Reporter Assay
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Reporter cell lines were created by transducing Jurkat (E6.1) T cells with Cignal Lenti NFAT reporter (Cat# CLS-015L), Cignal Lenti NFκB reporter (Cat# CLS-013L), or Cignal Lenti Negative Control (CLS-NCL) lentiviral particles purchased from Qiagen. An equal volume of Bright-Glo™ Luciferase Assay System (Promega) was added to an equal volume of cells. After 5 minutes, the cells were lysed, and the lysate was transferred to a black Costar EIA/RIA polystyrene half area 96-well plate (Corning). Luminescence was measured with the VICTOR™ X4 Multi-Plate Reader (PerkinElmer).
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