Anti mouse cd3 clone 17a2 efluor 660 antibody

Manufactured by Thermo Fisher Scientific

The Anti-mouse CD3 (clone 17A2)-eFluor 660 antibody is a fluorescent-labeled antibody that specifically binds to the CD3 antigen on mouse T cells. It is a tool for the identification and detection of mouse T cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cm1850 uv, by Leica (2 mentions) Antifade mounting medium with dapi, by Vector Laboratories (2 mentions) Tissue plus optimal cutting temperature compound, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-CD3 (512 citations) Anti-CD3 antibody (84 citations) Anti-mouse CD3 (43 citations) EFluor 660 (35 citations) CD3 (17A2, (21 citations) Anti-mouse CD3 antibody (11 citations) Anti-CD3 (17A2, (8 citations) Clone 17A2 (8 citations) Anti-CD3 (clone 17A2, (7 citations) CD3 (clone 17A2, (5 citations)

2 protocols using anti mouse cd3 clone 17a2 efluor 660 antibody

Lung Tissue Immunostaining and Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lungs were inflated with and fixed in paraformaldehyde-lysine-periodate fixative (PLP) overnight, placed in 30% sucrose solution in water for 4–8 hours, inflated with 30% Tissue Plus Optimal Cutting Temperature (OCT) compound (Fisher Scientific Cat. 23-730-571) in PBS, and frozen in OCT media at −80C.
Tissue sections were cut 20 μm thick in a cryostat (Leica CM 1850UV) at −20°C and stained with an anti-mouse CD3 (clone 17A2)-eFluor 660 antibody (ThermoFisher Scientific, cat: 50-0032-82) and Antifade Mounting Medium with DAPI (Vector Laboratories, cat: H-1200). Images were acquired using the EVOS FL AUTO 2.0 Imaging System at 10x magnification. Channels were overlayed using FIJI software (Schindelin et al., 2012 (link)).

Fitzgerald B., Connolly K.A., Cui C., Fagerberg E., Mariuzza D.L., Hornick N.I., Foster G.G., William I., Cheung J.F, & Joshi N.S. (2021). A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma. Cell Reports Methods, 1(5), 100080.

+ Open protocol

+ Expand

Immunofluorescence Staining of Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lungs were inflated with and fixed in paraformaldehyde-lysine-periodate fixative (PLP) overnight, placed in 30% sucrose solution in water for 4-8 hours, inflated with 30% Tissue Plus Optimal Cutting Temperature (OCT) compound (Fisher Scientific Cat. 23-730-571) in PBS, and frozen in OCT media at -80C.
Tissue sections were cut 20 μm thick in a cryostat (Leica CM 1850UV) at -20°C and stained with an anti-mouse CD3 (clone 17A2)-eFluor 660 antibody (ThermoFisher Scientific, cat: 50-0032-82) and Antifade Mounting Medium with DAPI (Vector Laboratories, cat: H-1200). Images were acquired using the EVOS FL AUTO 2.0 Imaging System at 10x magnification. Channels were overlayed using FIJI software (Schindelin et al., 2012 (link)).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!