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Antibody to vinculin

Manufactured by Merck Group
Sourced in United States

Antibody to vinculin is a laboratory reagent used for the detection and quantification of the vinculin protein, which is involved in cell-cell and cell-matrix adhesion. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of vinculin in biological samples.

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2 protocols using antibody to vinculin

1

Skeletal Muscle Signaling Pathway Analysis

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RT-PCR and immunoblotting were conducted as previously described.41 (link) Skeletal muscle underwent evaluation of Akt and AMPK activation using antibodies obtained from Cell Signaling Technology (Danvers, MA): p-308-Akt (#4056), p-473-Akt (#4058), total Akt (# 9272), p-172-AMPK (#2535), and GAPDH (# 51332S). Antibody to vinculin was obtained from Sigma (St. Louis, MO; SAB4200080).
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2

Oxidative Stress Signaling Pathways

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Apocynin (acetovanillone), PEG-catalase and ebselen were purchased from Sigma (USA). PDGF-BB and EGF were from Cell Signaling (USA). DPI, U0126, LY294002 were from Calbiochem (Germany). The primary antibodies to total Akt, phospho-Akt (Ser-473), phospho-Erk1/2 (Thr202/Tyr204), total Erk1/2, and secondary antibody were from Cell Signaling (USA). Antibody to Nox4, Duox1 and Duox 2 were from Novus Biologicals (USA), antibody to vinculin were from Sigma (USA). The expression plasmid encoding H2O2 biosensor HyPer fused to the nuclear export sequence (HyPer-NES) was from Evrogen, Russia. DCF-DA was kindly donated by Dr. M.Yu. Menshikov (Cardiology Research Center, Moscow, Russia).
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