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Helios pacific blue

Manufactured by BioLegend
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The Helios (Pacific Blue) is a flow cytometry instrument designed for the analysis of cellular samples. It is capable of detecting the Pacific Blue fluorescent dye.

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Lab products found in correlation

Lsrfortessa analyzer, by BD (2 mentions) Efluor 780, by Thermo Fisher Scientific (2 mentions) Recombinant human il 6, by R&D Systems (1 mentions) Facs fusion cell sorter, by BD (1 mentions) Tigit percp efluor710, by Thermo Fisher Scientific (1 mentions) Il 11, by R&D Systems (1 mentions) Pe efluor 610, by Thermo Fisher Scientific (1 mentions) Cd8 apc h7, by BD (1 mentions) Cd3 fitc, by BD (1 mentions) Cd25 pe cy7, by BD (1 mentions) Cd45ra fitc, by BD (1 mentions) Cd3 percp, by BD (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Hla dr fitc, by BD (1 mentions) Cd69 pe, by BD (1 mentions) Cd8 pe, by BD (1 mentions) Cd45 percp, by BD (1 mentions) Cd4 pe cy7, by BD (1 mentions) Cd25 apc, by BD (1 mentions) Trucount tube, by BD (1 mentions)

Close spelling variants of this product

Helios-Pacific Blue (22F6, (2 citations)

3 protocols using helios pacific blue

1

Phenotypic analysis of Treg subsets

Cited 1 time
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Cryopreserved PBMCs were thawed and stained with viability dye (eFluor 780; eBioscience). Antibodies against CD4 (FITC or V500), CD25 (APC), CD45RA (Alexa Fluor 700)(BD Biosciences), CD127 (PE-eFluor 610), FOXP3 (PE), TIGIT (PerCP-eFluor710) (eBioscience), Helios (Pacific Blue; Biolegend). Purified anti-FCRL3 antibody was provided by Nagata (37 (link)), and was detected with F(ab′)2 anti-mouse IgG (PE-Cy7; eBioscience). Flow cytometry analysis was performed on an LSR Fortessa analyzer (BD Biosciences), and sorting throughout this study was performed on a FACS Fusion cell sorter (BD Biosciences). Recombinant human IL-6, IL-27, CLC, IL-11 (R&D systems), and LIF (Peprotech) were added to suppression assays where indicated at the time of activation.
Bin Dhuban K., Bartolucci S., d'Hennezel E, & Piccirillo C.A. (2019). Signaling Through gp130 Compromises Suppressive Function in Human FOXP3+ Regulatory T Cells. Frontiers in Immunology, 10, 1532.
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2

Multiparametric Flow Cytometry Profiling

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Whole blood was drawn in EDTA tubes and the cells were incubated with fluorochrome-conjugated antibodies against human CD3-FITC, HLA-DR-FITC, CD45RA-FITC, CD8-PE, CD16+56-PE, CD69-PE, CD28-PE, CD3-PerCP, CD45-PerCP, CD45RO-PerCP-Cy5.5, CD4-PE-Cy7, CD25-PE-Cy7, CD25-APC, CD39-APC, CD62L-APC, CD8-APC-H7, CD4-APC-H7, CD3-Horizon V450, CD56-Horizon V450, all from BD Biosciences (San Jose, USA). Anti-Foxp3-PE was purchased from eBioscience (San Diego, USA) and Helios-Pacific Blue from Biolegend (San Diego, USA). Lymphocyte subpopulations from peripheral blood were measured by 4- or 7-color combinations. TruCount™ tubes (BD Biosciences) were used for assessment of absolute numbers (cells/μl) of leukocytes (CD45), and major lymphocyte populations; T lymphocyte (CD3), T helper (CD4), T cytotoxic (CD8), B (CD19) and NK (CD16+CD56) cells, as described by the manufacturer. Absolute numbers of subpopulations were derived from the major populations. Proportions of major populations were given as % of lymphocytes and proportions of subpopulations were given as % of their parent population.
Hjorth M., Dandu N, & Mellergård J. (2020). Treatment effects of fingolimod in multiple sclerosis: Selective changes in peripheral blood lymphocyte subsets. PLoS ONE, 15(2), e0228380.
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3

Ex vivo Flow Cytometry Analysis of PBMCs

Cited 1 time
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For ex vivo flow cytometry analysis, PBMCs were thawed and stained with viability dye (eFluor 780; eBioscience). Purified anti-FCRL3 (24 (link)) was used where mentioned, followed by staining with F(ab′)2 anti-mouse IgG (eFluor 660 or PE; eBioscience) and extensive washing with PBS. Cells were then stained with fluorochrome-conjugated Abs against CD4 (FITC or V500), CD25 (PE-CF594), CD45RA (Alexa Fluor 700) (BD Biosciences), CD127 (PE), TIGIT (PerCP–eFluor 710), FOXP3 (PE-Cy7), CD62L (FITC), HLA-DR (PE-Cy7), CTLA-4 (allophycocyanin) (eBioscience), and Helios (Pacific Blue; BioLegend).
For cytokine detection, PBMCs were incubated with PMA (25 ng/ml), ionomycin (1 μg/ml) (both from Sigma-Aldrich), and GolgiStop (eBioscience) for 4 h, followed by intracellular cytokine staining with Abs against IFN-γ (FITC), IL-2 (PerCP-Cy5.5), and IL-17 (allophycocyanin) (eBioscience).
Flow cytometry analysis was performed on an LSRFortessa analyzer, and sorting throughout this study was performed on a FACSAria IIu cell sorter (both from BD Biosciences).
Dhuban K.B., d’Hennezel E., Nashi E., Bar-Or A., Rieder S., Shevach E.M., Nagata S, & Piccirillo C.A. (2015). Coexpression of TIGIT and FCRL3 Identifies Helios+ Human Memory Regulatory T Cells. Journal of immunology (Baltimore, Md. : 1950), 194(8), 3687-3696.
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