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Gant61

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GANT61 is a small molecule inhibitor that targets the G2 and S phase-expressed nuclear antigen (GANT61) protein. It functions by disrupting the transcriptional activity of the GLI family of zinc finger transcription factors, which are key mediators of the Hedgehog signaling pathway.

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Lab products found in correlation

Hoechst, by Merck Group (1 mentions) Icrt3, by Merck Group (1 mentions) Verteporfin, by Bio-Techne (1 mentions) In cell analyser 2200, by GE Healthcare (1 mentions) In cell analyser workstation software, by GE Healthcare (1 mentions) Incucyte zoom cell imaging system, by Sartorius (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Skbr3, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) C3h10t1 2, by American Type Culture Collection (1 mentions) Cyclopamine, by R&D Systems (1 mentions) Cytochalasin d, by MP Biomedicals (1 mentions) Xav939, by Selleck Chemicals (1 mentions) Cyclopamine, by Selleck Chemicals (1 mentions) Iwr 1 endo, by Santa Cruz Biotechnology (1 mentions) Pkf118 310, by Merck Group (1 mentions) Iwp 2, by Santa Cruz Biotechnology (1 mentions) Icrt14, by R&D Systems (1 mentions) Iwr1 exo, by Santa Cruz Biotechnology (1 mentions) Lde225, by Selleck Chemicals (1 mentions)

4 protocols using gant61

1

Pathway Agonist and Inhibitor Screening

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The signalling pathway agonists/inhibitors Verteporfin (Tocris); iCRT3 (Sigma); DAPT (Merck Millipore) and GANT61(R and D Systems) were applied at the concentrations indicated during a 7-day incubation following 24-hour transduction of GSK3α/β floxed cells with Cre recombinase expressing lentivirus. Cells were stained with Hoechst (Sigma) at 1 µg/ml, imaged, using an IN Cell analyser 2200 (GE Healthcare) and cell number determined using IN Cell analyser workstation software (GE Healthcare).
Live cell imaging was carried out 5–7 days after viral transduction using an IncuCyte ZOOM cell imaging system (Essen Biosciences).
Hurcombe J.A., Hartley P., Lay A.C., Ni L., Bedford J.J., Leader J.P., Singh S., Murphy A., Scudamore C.L., Marquez E., Barrington A.F., Pinto V., Marchetti M., Wong L.F., Uney J., Saleem M.A., Mathieson P.W., Patel S., Walker R.J., Woodgett J.R., Quaggin S.E., Welsh G.I, & Coward R.J. (2019). Podocyte GSK3 is an evolutionarily conserved critical regulator of kidney function. Nature Communications, 10, 403.
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2

Culturing Breast Cancer Cell Lines

Cited 1 time
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The SUM149, SUM159 and SUM190 cell lines were obtained from Asterand Inc (Detroit, MI) and cultured per manufacturer’s instructions and as described previously [35 (link)]. MDA-MB-231, SKBR3 cells and C3H10T1/2 cell lines were from American Type Culture Collection (ATCC) (Manassas, VA) and cultured per their instructions. Human mammary epithelial cells (HMEC) were obtained from Lonza Group Ltd. (Allendale, NJ) and cultured per manufacturer’s instructions using their Mammary Epithelial Growth Medium Kit (CC-3150). GANT58 (Cat # 3889), GANT61 (Cat # 3191), JK184 (Cat # 3341) and cyclopamine were obtained from Tocris/R&D Systems (Minneapolis, MN). GANT61 in gram quantities was from DC Chemicals (Shanghai, China). HPI-1/2/3/4 were a kind gift from Dr. James K. Chen, Stanford School of Medicine. HPI-1/3/4 were also purchased from Sigma Aldrich (St. Louis, MO). Cytochalasin D was from MP Biomedicals, LLC (Solon, OH). Hoechst-33342 and YOYO-1 dyes were from Thermo Fisher Scientific
Oladapo H.O., Tarpley M., Sauer S.J., Addo K.A., Ingram S.M., Strepay D., Ehe B.K., Chdid L., Trinkler M., Roques J.R., Darr D.B., Fleming J.M., Devi G.R, & Williams K.P. (2017). Pharmacological targeting of GLI1 inhibits proliferation, tumor emboli formation and in vivo tumor growth of inflammatory breast cancer cells. Cancer letters, 411, 136-149.
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Investigating Signaling Pathway Inhibitors in MCL

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Cells and clinical information from MCL patients described in this manuscript (Additional file 4: Table S2) were collected and published with the written informed consent of each patient under The University of Texas MD Anderson Cancer Center IRB-approved clinical protocol LAB08-0190 for use of human tissues.
The following agents were tested: Wnt inhibitors XAV939 (Selleck Chemicals, Houston, TX), iCRT14 (R&D Systems, Minneapolis, MN), CCT036477, PKF118-310 (Sigma-Aldrich, St. Louis, MO), IWP2, IWR1-endo, and IWR1-exo (Santa Cruz Biotechnologies, Santa Cruz, CA); Hedgehog inhibitors GANT61 (R&D Systems, Minneapolis, MN), LDE225 and Cyclopamine (both from Selleck Chemicals, Houston, TX); Notch inhibitor RO4929097 (Selleck Chemicals, Houston, TX).
Mathur R., Sehgal L., Braun F.K., Berkova Z., Romaguerra J., Wang M., Rodriguez M.A., Fayad L., Neelapu S.S, & Samaniego F. (2015). Targeting Wnt pathway in mantle cell lymphoma-initiating cells. Journal of Hematology & Oncology, 8, 63.
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4

GANT61 Treatment of iKRAS Cells

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Cells were treated with 5 μM of GANT61 (3191/10; R and D Systems) for 3–5 days prior to collection of RNA or protein lysates. Alternatively, iKRAS cells were plated at 150,000 cells with or without Dox (1 μg/mL) in the presence of absence of 5 μM GANT61 and RNA was collected 72 hr post-plating.
Adams C.R., Htwe H.H., Marsh T., Wang A.L., Montoya M.L., Subbaraj L., Tward A.D., Bardeesy N, & Perera R.M. (2019). Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer. eLife, 8, e45313.
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