Human il 1β elisa kit 2

Manufactured by BD

The BD Human IL-1β ELISA kit II is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure interleukin-1 beta (IL-1β) levels in human serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Mouse il 1β elisa kit, by BD (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Synergy mx, by Agilent Technologies (1 mentions)

2 protocols using human il 1β elisa kit 2

Cytokine Profiling in MAYV Infection

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For in vitro cytokine determination, BMDMs were seeded overnight at a density of 2 × 10⁵ cells/well in 48-well plates and prestimulated with 300 ng/ml of PAM(3)CSK(4) (Invivogen) for 4 h, and subsequently infected with MAYV. The cytokines in the supernatants were assayed using a mouse IL-1β ELISA kit (BD Biosciences) according to the manufacturer’s instructions. For in vivo cytokine determination, footpads were processed and the supernatants from total homogenates were obtained. The levels of IL-1β and IL-18 in the homogenates were detected using a mouse IL-1β ELISA kit (BD Biosciences) and a mouse IL-18 ELISA kit (RD-MBL), respectively, according to the manufacturer’s instructions. Detection of IL-1β, IL-18 and Caspase 1 (p20 subunit) in human serum samples was accomplished by using human IL-1β ELISA kit II (BD Biosciences), human total IL-18 (RD) and human caspase-1 ELISA kit (RD) respectively.

de Castro-Jorge L.A., de Carvalho R.V., Klein T.M., Hiroki C.H., Lopes A.H., Guimarães R.M., Fumagalli M.J., Floriano V.G., Agostinho M.R., Slhessarenko R.D., Ramalho F.S., Cunha T.M., Cunha F.Q., da Fonseca B.A, & Zamboni D.S. (2019). The NLRP3 inflammasome is involved with the pathogenesis of Mayaro virus. PLoS Pathogens, 15(9), e1007934.

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Quantifying IL-1β Secretion Using ELISA

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Samples from at least three separate experiments were collected for each infection and treatment condition. Samples were cleared of cellular debris by centrifugation at 800 × g for 5 min and cell-free supernatants were used for IL-1β ELISA assay. IL-1β secretion levels were measured using Human IL-1β ELISA kit II (BD Biosciences). ELISAs were performed as per manufacturer's instructions. Briefly, samples, controls, and standards were diluted with assay diluent, loaded onto a 96-well plate, and incubated for 2 h at room temperature. Wells were washed and incubated with the detection antibody for 1 h at room temperature. After washing, substrate was added to all wells. The reactions were stopped with stop solution and optical densities were read at an absorbance of 450 nm using a Synergy MX plate reader (BioTek Instruments). P-values were calculated using two-tailed Student's t-test.

Johnson L., Atanasova K.R., Bui P.Q., Lee J., Hung S.C., Yilmaz Ö, & Ojcius D.M. (2015). Porphyromonas gingivalis attenuates ATP-mediated inflammasome activation and HMGB1 release through expression of a nucleoside-diphosphate kinase. Microbes and infection / Institut Pasteur, 17(5), 369-377.

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