Pcie 6353

Manufactured by National Instruments

Sourced in United States

The PCIe-6353 is a high-performance multifunction data acquisition (DAQ) device from National Instruments. It features 16 analog inputs, two analog outputs, four digital I/O lines, and four counter/timer channels. The device utilizes the PCIe interface for high-speed data transfer. The core function of the PCIe-6353 is to provide data acquisition and control capabilities for various applications.

Automatically generated - may contain errors

Lab products found in correlation

12 protocols using pcie 6353

Automated Microscopy Hardware Control and etSTED Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microscope hardware control is mainly performed through a National Instruments data acquisition (NI-DAQ) acquisition board (PCIe-6353, National Instruments). Hardware is controlled using microscope control software ImSwitch^{19 (link)} written in Python. Control of the etSTED method is performed using a custom-written widget and controller in ImSwitch, available at GitHub (https://github.com/kasasxav/ImSwitch and https://github.com/jonatanalvelid/ImSwitch-etSTED), which controls lasers, image acquisition, and runs real-time analysis pipelines with customizable parameters. Instructions on how to run etSTED imaging can be found in the GitHub repository of the standalone widget (https://github.com/jonatanalvelid/etSTED-widget), while instructions on how to run ImSwitch can be found in the repository on GitHub and corresponding documentation (https://imswitch.readthedocs.io).
A focus lock controlled with ImSwitch combining an infrared laser (CP980S, Thorlabs), a CMOS camera (DMK 33UP1300, The Imaging Source) and the z-piezostage through a feedback loop, as previously described^{5 (link)}, is used. It enables experiments to run stably for time periods longer than hours.
The microscope control computer contains a Ryzen 7 3700X CPU (AMD) and a GeForce RTX 3060 Ti GPU (ASUS).

Alvelid J., Damenti M., Sgattoni C, & Testa I. (2022). Event-triggered STED imaging. Nature Methods, 19(10), 1268-1275.

+ Open protocol

+ Expand

Eye-Tracking in Visual Perception Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

During the Learning session, eye-position data of the right eye were acquired with a desktop-mounted Eyelink 1000 eye tracker (SR Research, Mississauga, Ontario, Canada) at a sampling rate of 1000 Hz. The output of the eye tracker was recorded with a data acquisition board (PCIe-6353, National Instruments, USA) interfaced through a shielded I/O connector block (SCB-68, National Instruments, USA). The visual images were presented via a 27-inch monitor (1920 × 1080 resolution, 60 Hz refresh rate).

Kang J., Kim H., Hwang S.H., Han M., Lee S.H, & Kim H.F. (2021). Primate ventral striatum maintains neural representations of the value of previously rewarded objects for habitual seeking. Nature Communications, 12, 2100.

+ Open protocol

+ Expand

Precise Button Response Tracking

Check if the same lab product or an alternative is used in the 5 most similar protocols

Participants were given a custom-built response box, composed of a single button. Button presses were sent to the IO board (National instruments, PCIe-6353) and are therefore recorded with a sub-millisecond precision.

Lawlor J., Zagala A., Jamali S, & Boubenec Y. (2023). Pupillary dynamics reflect the impact of temporal expectation on detection strategy. iScience, 26(2), 106000.

+ Open protocol

+ Expand

Wireless Inertial Measurement in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rats were equipped with the wireless IMU and placed inside a circular arena (118 cm diameter) with transparent plastic walls (30 cm high). The sensitivity of the IMU was configured remotely from the software interface (accelerometers: ±16 g; gyroscopes: ±1000°/s) and the acquisition of IMU data was initiated. A 25 ms analog waveform with uniform white noise distribution was generated under LabVIEW and played at random intervals (5–15 s) through the analog output of a DAQ board (PCIe-6353, National Instruments) connected to a loudspeaker via a custom amplifier (Fig. 3A, sound amplitude: 90–110 dBA). A square 5 V waveform with the same duration was played simultaneously via a second analog output channel, and fed to the gate input of the IR LED controller. A total of 30 acoustic stimuli were delivered per rat.

Pasquet M.O., Tihy M., Gourgeon A., Pompili M.N., Godsil B.P., Léna C, & Dugué G.P. (2016). Wireless inertial measurement of head kinematics in freely-moving rats. Scientific Reports, 6, 35689.

+ Open protocol

+ Expand

Simultaneous Neural and EMG Recording Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neural activity was recorded simultaneously in both IOS and 2PLSM as the differential potentials between the two leads of either the PFA-coated tungsten microwires (#795500, A-M Systems, Sequim, WA) (Huo et al., 2014 (link); Winder et al., 2017 (link)) for cortical and hippocampal stereotrodes. EMG activity was identically recorded with PFA-coated 7-strand stainless-steel microwires (#793200, A-M systems, Sequim, WA). Stereotrode tungsten microwires were threaded through polyimide tubing (#822200, A-M Systems, Sequim, WA) giving an interelectrode spacing of ~100 µm. The tungsten microwires were crimped to gold pin connectors, with impedances typically between 70 and 120 kΩ at 1 kHz. EMG stainless-steel microwires were fabricated in a similar fashion, but with an interelectrode spacing of several mm. Each signal was amplified and hardware bandpass filtered between 0.1 Hz and 10 kHz (DAM80, World Precision Instruments, Sarasota, FL) and then digitized at 20 kHz (PCIe-6341 for IOS experiments, PCIe-6321 and PCIe-6353 for 2PLSM experiments, National Instruments, Austin, TX).

Turner K.L., Gheres K.W., Proctor E.A, & Drew P.J. (2020). Neurovascular coupling and bilateral connectivity during NREM and REM sleep. eLife, 9, e62071.

+ Open protocol

+ Expand

LabVIEW-driven Stimulation and Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols

All stimuli were controlled with and data were acquired by programs written in LabVIEW (version 16.0; National Instruments). Data were digitized by an analog-to-digital converter and sampled at 50 μs intervals (PCIe-6353, National Instruments). Stimulation and control signals were generated with a digital-to-analog converter (PCI-6733, National Instruments).

Azimzadeh J.B., Fabella B.A., Kastan N.R, & Hudspeth A.J. (2018). Thermal excitation of the mechanotransduction apparatus of hair cells. Neuron, 97(3), 586-595.e4.

+ Open protocol

+ Expand

STED-Anisotropy Measurement System Setup

Check if the same lab product or an alternative is used in the 5 most similar protocols

A home-built setup was used for STED-anisotropy measurements. CW laser at 488 nm, generated by an OBIS LX 120 mW CW laser (Coherent), was passed through Glan–Taylor polarizer (Thorlabs) to obtain highly pure (100,000:1) linearly polarized excitation beam. CW laser at 592 nm (VFL-P-1000 592; MPB Communications Inc.) was passed through a vortex phase plate (VPP-1a; RPC Photonics) to generate a depletion with a doughnut profile. The excitation and depletion beams were focused on the sample using a 100×, 1.49 NA oil immersion objective (Nikon). Excitation modulation and synchronous detection were carried out according to SI Appendix, section 3.
The emitted fluorescence was collected by the same objective lens. The parallel and orthogonal components of the emitted fluorescence were separated by a polarizing cube (CCM1-PBS251; Thorlabs) and steered to 2 single-photon counters (SPCM-AQRH; EXCELITAS) for anisotropy measurements. The TTL signal from SPC was acquired with a DAQ card (PCIe-6353; National Instruments). Data acquisition and analysis was automated by custom LabVIEW software (National Instruments).

Park S.J., Shakya A, & King J.T. (2019). Depletion layer dynamics of polyelectrolyte solutions under Poiseuille flow. Proceedings of the National Academy of Sciences of the United States of America, 116(33), 16256-16261.

+ Open protocol

+ Expand

Eye-Tracking and Joystick-Controlled Visual Task

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eye-position data were acquired using the Oculomatic Pro 1000 eye tracker (Bio-Signal, Texas, USA) with a sampling rate of 1000 Hz. To manipulate the cursor on the screen, a 2-axis joystick was used (HF22S10 model, APEM). The data output from both the eye tracker and joystick were recorded using a data acquisition board (PCIe-6353, National Instruments, USA) interfaced through a shielded I/O connector block (SCB-68, National Instruments, USA).
Visual stimuli were presented via a 27-inch monitor (1920 × 1080 resolution, 240 Hz refresh rate). All behavioral tasks were controlled by a custom behavior-controlling system (Blip software; available at www.cocila.net/blip).

Paeng S, & Kim H.F. (2024). Gaze patterns reflect the retrieval and selection of memories in a context-dependent object location retrieval task. Scientific Reports, 14, 9433.

+ Open protocol

+ Expand

High-Precision Eye Tracking Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eye position data were acquired using the Oculomatic Pro 1000 eye tracker (Bio-Signal, Texas, USA) at a sampling rate of 1,000 Hz. The output of the eye tracker was recorded with a data acquisition board (PCIe-6353, National Instruments, USA) interfaced through a shielded I/O connector block (SCB-68, National Instruments, USA). The visual images were presented via a 27-inch monitor (1920 × 1080 resolution, 240 Hz refresh rate). All behavioral tasks were controlled by a custom behavior controlling system (Blip; available at www.cocila.net/blip).

Hwang S.H., Ra Y., Paeng S, & Kim H.F. (2022). Motivational salience drives habitual gazes during value memory retention and facilitates relearning of forgotten value. iScience, 25(10), 105104.

+ Open protocol

+ Expand

Single-cell Electrophysiology of Cultured Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

For single-cell electrophysiological recording, cultured neurons were incubated in Tyrode’s buffer containing 20 μM gabazine, 10 μM NBQX, and 25 μM APV (Tyrode’s buffer containing 50 nM 2-APB for HEK293T cells). The electrophysiological experiments were conducted at room temperature. Borosilicate glass electrodes (Sutter) were pulled to a tip resistance of 2.5 to 5 MΩ. The internal solution containing 125 mM potassium gluconate, 8 mM NaCl, 0.6 mM MgCl₂, 0.1 mM CaCl₂, 1 mM EGTA, 10 mM HEPES, 4 mM Mg-ATP, and 0.4 mM GTP·Na₂ (pH 7.3) was adjusted to 295 mOsm/kg with 1 M sucrose and injected into the glass electrode. The glass electrode’s position was adjusted by a Sutter MP285 micromanipulator. An Axopatch 200B (Axon Instruments) amplifier was utilized to clamp the cells. Membrane potential data recorded from the amplifier were filtered by an internal 5-kHz Bessel filter and digitalized at 9681.48 Hz with a National Instruments PCIe-6353 data acquisition (DAQ) board.

Liu S., Ling J., Chen P., Cao C., Peng L., Zhang Y., Ji G., Guo Y., Chen P.R., Zou P, & Chen Z. (2023). Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes. Proceedings of the National Academy of Sciences of the United States of America, 120(34), e2306950120.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!