The Qingrekasen Granule was purchased from Xinjiang Uygur Pharmaceutical Co., Ltd. (NO.1807521, Xinjiang, China). Adriamycin (ADR) was purchased from Shenzhen Main Luck Pharmaceuticals Inc. (No. 2007E1, Shenzhen, China), and Benazepril was purchased from Novartis (No. 2007, Beijing, China). The BCA Protein Assay Kit was obtained from Beyotime Biotech Inc. (P0012, Shanghai, China), and Guangzhou Jet Bio-Filtration Co., Ltd. (Guangzhou, China) provided the Elisa plates (12 well strip ×8). HPLC grade methanol and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). Anti-PI3K and anti-p-PI3K antibodies were purchased from Cell Signaling Technology Inc. (MA, United States). The Anti-AKT, anti-p-AKT, anti-mTOR, anti-P-mTOR, anti-BCL-2, anti-Caspase-3, and Beta-actin antibodies were purchased from Bioworld Technology Inc. (MN, United States). All other reagents were HPLC grade.
Anti p akt
Manufactured by Bioworld Technology
Sourced in United States, China
Anti-p-AKT is a primary antibody that recognizes the phosphorylated form of the AKT protein. AKT is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes, including metabolism, cell proliferation, cell survival, and cell growth. The phosphorylation of AKT is a critical step in the activation of this signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti akt, by Bioworld Technology (7 mentions)
Anti bcl 2, by Bioworld Technology (3 mentions)
Anti p erk, by Bioworld Technology (3 mentions)
Anti erk, by Bioworld Technology (3 mentions)
Anti mtor, by Bioworld Technology (2 mentions)
Anti p mtor, by Bioworld Technology (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Anti bax, by Bioworld Technology (2 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Anti caspase 3, by Bioworld Technology (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Anti pi3k, by Cell Signaling Technology (1 mentions)
Anti p pi3k, by Cell Signaling Technology (1 mentions)
Benazepril, by Novartis (1 mentions)
Anti gsk 3β, by Bioworld Technology (1 mentions)
Anti p21, by Bioworld Technology (1 mentions)
Monoclonal anti α tubulin antibody, by Merck Group (1 mentions)
Anti p27, by Bioworld Technology (1 mentions)
Anti tle3, by Abcam (1 mentions)
9 protocols using anti p akt
1
Qingrekasen Granule Anticancer Mechanism
2
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We carried out western blot as previously described [33 (link)], using anti-TLE3 (Abcam, Cambridge, MA, USA), anti-FOXO3, anti-Akt, anti-GSK-3β, anti-ERK, anti-p21, anti-p27, anti-p-FOXO3, anti-p-Akt, anti-p-GSK-3β and anti-p-ERK (Bioworld Technology, St. Louis Park, MN, USA) to detect the corresponding proteins. Anti-α-Tubulin monoclonal antibody (Sigma, St. Louis, MO, USA) served as a loading control.
3
Geniposide Modulates Alzheimer's Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Geniposide (purity> 98%) was obtained from MedChem Express. All antibodies, including anti-Aβ1-40, anti-LC3-II, anti-Beclin1, anti-Akt, anti-p-Akt, anti-mTOR, anti-p-mTOR, anti-4E-BP1, and anti-p-4E-BP1, were purchased from Bioworld Technology Inc. (Nanjing, China). ELISA kits for Aβ1-40 and Aβ1-42, and BCA protein assay reagents were purchased from Boster Biotechnology Co., Ltd. (Wuhan, China). All chemicals were of reagent grade.
4
Protein Expression Analysis in Cell and Tissue Lysates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the experiments were completed, the C2C12 myotubes, mice skeletal muscle, and liver tissue lysates were prepared using a standard protocol. In brief, the cell and tissue samples were mixed with sample buffer (250 mM tris-hydrochloride (pH 6.8), 0.5 M dithiothreitol (DTT), 10% sodium dodecyl sulfate (SDS), 0.5% bromophenol blue, 50% glycerol, and 5% 2-mercaptoethanol) and denatured at 95°C for 5 min. Sample proteins (50 µg) were separated via 10% SDS-polyacrylamide gel electrophoresis (PAGE) and electrotransferred to nitrocellulose transfer membranes (Whatman, 401396, Dassel, Germany), which were incubated overnight at 4°C with 5% skim milk or 5% bovine serum albumin and a range of antibodies. Anti-AMPK, anti-phospho-(p)-AMPK, anti-AKT, anti-p-AKT, anti-GLUT4 (Bioworld Technology), and anti-β-actin (Bethyl Laboratories) antibodies were the primary antibodies used, and a horseradish peroxidase- (HRP-) conjugated goat anti-rabbit immunoglobulin G (IgG) (Santa Cruz Biotechnology) was used as the secondary antibody. The antigen-antibody reaction was detected using a SuperSignal West Femto maximum sensitivity substrate (Thermo Fisher Scientific, Rockford, IL, USA).
5
PI3K/Akt/MAPK Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Roswell Park Memorial Institute (RPMI 1640) culture medium, the glucose assay kit and the radioimmunoprecipitation assay (RIPA) lysis solution were bought from Beijing Solarbio Science & Technology Co., Ltd., (Beijing, China). Protein bands were visualized with an enhanced chemiluminescence (ECL) detection kit (Thermo Fisher Scientific, Waltham, MA, USA). Anti-P-PI3K, anti-P-Akt, anti-P-MAPK and anti-GAPDH primary antibodies were acquired from bioWorld Technology Inc., (Dublin, OH, USA). Secondary antibodies were purchased from Zhongshanjinqiao Biotech (Beijing, China). Polyvinylidene fluoride (PVDF) transfer membranes were obtained from EMD Millipore.
6
Endothelial Cell Migration and Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum, Trizol, Lipofectamine 2000and HRP-conjugated mouse and rabbit secondary antibodies were obtained from Invitrogen (Shanghai, China). Endothelial Cell Medium (ECM) was obtained from ScienCell (San Diego, USA). The anti-AXIIR and anti-CD31antibody were obtained from Santa Cruz Biotechnology Co., Ltd. (Dallas, USA). Antibodies to MMP2, MMP9 were obtained from Shanghai RuianBioTechnologies (Shanghai, China). The anti-AKT, anti-p-AKT, anti-ERK, anti-p-ERK, anti-TIMP2 antibodies were purchased from Bioworld Technology, Inc. (Nanjing, China). The anti-GAPDH antibody was obtained from Tianjin sungene Biotech (Tianjin, China). Matrigel Matrix was obtained from BD Biosciences (San Jose, USA). Cell-light EdU DNA cell proliferation kit was obtained from RiboBio (Guangzhou, China). PrimeScript RT reagents Kit, PrimeSTAR® HS DNA Polymerase with GC Buffer and SYBR Premix Ex Taq were obtained from TAKARA BIOTECHNOLOGY (Dalian, China). Annexin V-FITC Apoptosis Detection Kit was obtained from eBioscience (San Diego, CA, USA). The transwell chamber and other cell culture plates were obtained from Corning Inc (NY, USA). All other regents were of analytical grade.
7
Alpinetin's Anticancer Effects on Lung Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lung cancer cell lines A549, SK-MES-1, NCI-H292, and A549/cis-diammined dichloridoplatium (CDDP) were purchased from the Shanghai Institute of Cell Biology (Shanghai, People’s Republic of China), Chinese Academy of Science. The study was approved by the Ethics Committee of Shengjing Hospital of China.
Alpinetin was purchased from the National Institutes for Food and Drug Control (Beijing, People’s Republic of China). Fetal bovine serum, RPMI-1640 medium, and trypsin were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Dimethyl sulfoxide, thiazolyl blue tetrazolium bromide (MTT), and Hoechst 33258 were purchased from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). A dead cell apoptosis kit with annexin V-FITC and propidium iodide (PI) was purchased from Sigma-Aldrich Co., St Louis, MO, USA, and a transcription kit was purchased from Fermentas (Waltham, MA, USA).
Anti-Bcl-2, anti-Bcl-xL, anti-Bax, anti-XIAP, anticyto-chrome c, anti-PI3K, anti-Akt, anti-p-Akt, anti-MPR1, anti-MPR5, and anti-P-gp were all purchased from Bioworld (MN, USA). All of the secondary antibodies were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. (Beijing, People’s Republic of China), and an EPICS ALTRA flow cytometer was purchased from Beckman Coulter (Brea, FL, USA).
Alpinetin was purchased from the National Institutes for Food and Drug Control (Beijing, People’s Republic of China). Fetal bovine serum, RPMI-1640 medium, and trypsin were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Dimethyl sulfoxide, thiazolyl blue tetrazolium bromide (MTT), and Hoechst 33258 were purchased from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). A dead cell apoptosis kit with annexin V-FITC and propidium iodide (PI) was purchased from Sigma-Aldrich Co., St Louis, MO, USA, and a transcription kit was purchased from Fermentas (Waltham, MA, USA).
Anti-Bcl-2, anti-Bcl-xL, anti-Bax, anti-XIAP, anticyto-chrome c, anti-PI3K, anti-Akt, anti-p-Akt, anti-MPR1, anti-MPR5, and anti-P-gp were all purchased from Bioworld (MN, USA). All of the secondary antibodies were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. (Beijing, People’s Republic of China), and an EPICS ALTRA flow cytometer was purchased from Beckman Coulter (Brea, FL, USA).
8
Hippocampal Immunohistochemistry for BDNF, p-Akt, Bcl-2, and Bax
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hippocampal tissues were fixed, embedded in paraffin, and sectioned at 5 μm. Immunohistochemistry for BDNF, p-Akt, Bcl-2, and Bax was then carried out on the sections. Slices were incubated with the primary antibodies anti-BDNF, anti-p-Akt, anti-Bcl-2, or anti-Bax (1 : 200, Bioworld, USA) overnight at 4°C. The sections were then incubated with secondary antibodies and visualized using diaminobenzidine (DAB) as the chromagen. Cells with brown granules were considered immunoreactive positive cells. The slides were observed under a microscope and photographed.
9
Western Blot Analysis of Spinal Cord Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spinal cords were homogenized with a micro content motor-operated tissue homogenizer (Kimble Kontes, USA), using protein extraction kit (Sigma, USA) supplemented with a cocktail of protease inhibitors. The homogenates were centrifuged at 13,000g for 20 min at 4 ℃, and the supernatants were collected. The protein concentration was determined by BCA method. Equal amounts of protein (30 μg) were separated by SDS-PAGE and electroblotted onto PVDF membrane (Immobilon-P, Millipore, USA). After non-specific binding was blocked with 5% non-fat dry milk, membranes were incubated at 4 ℃ overnight with anti-MBP, anti-Akt, anti-ERK, anti-JNK, anti-P-Akt, anti-P-ERK, anti-P-JNK and anti-β-actin (1:1000, Bioworld, USA). After washing the membrane the next day, it should be incubated with the second antibody at room temperature for 2 hours. Bands were visualized with an enhanced chemiluminescence (ECL) system (GE Healthcare Life Sciences, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!