Phospho perk ma5 15033

Recombinant mouse leptin (L3772), N-acetyl cysteine (A9165), Ac-YVAD-CMK (SML0429), DPI (CN-240-0010), 3-methyladenine (M9281), type IV collagenase (C5138), type I collagen (C3867), and CA-074-Me (205530) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bafilomycin A1 (1829-50) and tauroursodeoxycholic acid (TUDCA, HY-19696A) were obtained from Biovision (Milipitas, CA, USA) and Medchem Express (New Jersey, USA), respectively. The primary antibodies against BiP (3183), ATF6 (65880), total PERK (3192), total eif2α (9722s), phospho-eif2α (9721s), beclin-1 (3738), LC3 (2775), p62 (5114), cleaved caspase-3 (9664), pro-caspase-3 (9662), Gasdermin D (39754), and IL-1β (12507) were purchased from Cell Signaling Technologies (Danvers, MA, USA); caspase-1 (AG-20B-0042) and ASC (AG-25B-0006) were procured from Adipogen (San Diego, CA, USA); Phospho-PERK (MA5-15033) and ATG5 (PA1-46178) were purchased from Thermo Fisher (Rockford, IL, USA); NOX1 (NBP1-31546), NLRP3 (MAB7578), and Cathepsin B (ab58802) were obtained from Novusbio (Littleton, CO, USA), R&D systems (Minneapolis, MN, USA), and Abcam (Cambridge, UK), respectively.

Each of the primary antibodies were used according to the manufacturer’s protocol: Phospho-JNK #4671, JNK #9252, IRE1α #3294, PERK #5683, phospho-p38 MAPK #9215, p38 MAPK #9212, phospho-p44/42 MAPK #9106, p44/42 MAPK #4695, (Cell Signaling, Danvers, MA). phospho- IRE1α #PA1-16927, phospho-PERK #MA5-15033, and HCV core (Thermo Scientific, Rockford, IL). ATF6 #ab11909 (Abcam, Cambridge, MA).