Tissues were processed using methods described previously26 . 200,000 cells were stained for 30 minutes (1:100) to antibodies: CCR2-PE (RnD Systems cat no.FAB5538P), CD24-Brilliant Violet (BD Pharmingen cat no.562563), F4/80-PE (Serotec cat no.MCA497PE), CD69-FITC (Biolegend cat no.104505), CD4-PE-Cy5 (BD Pharmingen cat no.553654), CD8-APC (Biolegend cat no.100711), CD11b-APC-Cy7 (BD Pharmingen cat no.557657), human anti-CD40-FITC (R&D Systems, cat no.MAB6321) or murine anti-CD40-FITC (BD Biosciences cat no.12040181). Cells were analyzed using a BD LSR II flow cytometer. Compensation controls were performed to minimize spectral overlap artifacts. Isotype control antibodies were utilized for background correction.
Murine anti cd40 fitc
Manufactured by BD
Murine anti-CD40-FITC is a fluorescently labeled antibody that binds to the CD40 receptor on the surface of cells. It can be used in flow cytometry applications to detect and analyze CD40-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd8 apc, by BioLegend (2 mentions)
Cd11b apc cy7, by BD (2 mentions)
F4 80 pe, by Bio-Rad (2 mentions)
Cd69 fitc, by BioLegend (2 mentions)
Cd4 pe cy5, by BD (2 mentions)
Ccr2 pe, by RnD Systems (2 mentions)
Cd24 brilliant violet, by BD (2 mentions)
Human anti cd40 fitc, by R&D Systems (2 mentions)
Lsr 2 flow cytometer, by BD (2 mentions)
2 protocols using murine anti cd40 fitc
1
Multiparameter Flow Cytometry Analysis
2
Multiparameter Flow Cytometry Analysis
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Tissues were processed using methods described previously26 . 200,000 cells were stained for 30 minutes (1:100) to antibodies: CCR2-PE (RnD Systems cat no.FAB5538P), CD24-Brilliant Violet (BD Pharmingen cat no.562563), F4/80-PE (Serotec cat no.MCA497PE), CD69-FITC (Biolegend cat no.104505), CD4-PE-Cy5 (BD Pharmingen cat no.553654), CD8-APC (Biolegend cat no.100711), CD11b-APC-Cy7 (BD Pharmingen cat no.557657), human anti-CD40-FITC (R&D Systems, cat no.MAB6321) or murine anti-CD40-FITC (BD Biosciences cat no.12040181). Cells were analyzed using a BD LSR II flow cytometer. Compensation controls were performed to minimize spectral overlap artifacts. Isotype control antibodies were utilized for background correction.
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