Facs aria model
The BD FACS Aria is a high-performance cell sorter designed for research applications. It is capable of multi-parameter analysis and sorting of cells or particles based on their physical and fluorescent characteristics. The core function of the FACS Aria is to provide users with a powerful tool for cell sorting and analysis.
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7 protocols using facs aria model
Isolating and Characterizing Murine BMSC
Isolation and Differentiation of Mouse and Human BMSC
Here, we find out that the mouse BMSC were sorted as CD29+Sca‐1+CD45−CD11b‐, while human BMSC (hBMSC) were sorted as CD146+STRO‐1+CD45‐. Then, we gathered and cultured them for 1‐2 weeks. In culture flasks, the primary BMSC were separated and seeded for cell population enrichment. Approximately 1 week later, as the second‐passage BMSC reached clustered, they were subcultured. Afterwards, we induced adipogenic and osteogenic differentiation in the third‐passage BMSC. Plasmid transfection also executed in third‐passage BMSC.
Isolation and Purification of Intestinal Stem Cells
Isolation and Characterization of Murine Mesenchymal Stem Cells
Isolation and Culture of Mouse BMSCs
The sorted Sca-1+CD29+CD45−CD11b− cells were collected and plated into 6-well plates (1,000 cells per well). After 4 h of adhesion, unattached cells were removed. Medium was changed every 3 days, and cultures were fixed and stained with 0.5 mg ml−1 of crystal violet on day 10. Colonies containing 50 or more cells were counted.
Isolation and Characterization of Murine Mesenchymal Stem Cells
Isolation of Mouse and Human SSCs
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