Jem 1200 exii transmission electron microscope

Manufactured by JEOL

Sourced in Japan

The JEM-1200 EXII is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of materials at the nanoscale level. The core function of the JEM-1200 EXII is to generate and transmit a beam of electrons through a thin specimen, creating a magnified image that can be observed and analyzed.

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Lab products found in correlation

Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Ammonium hydroxide, by Electron Microscopy Sciences (2 mentions) Formvar carbon coated electron microscopy grids, by Electron Microscopy Sciences (2 mentions) Orca hr digital camera, by Hamamatsu Photonics (2 mentions) Amt image capture engine, by Advanced Microscopy Techniques (2 mentions) Ultracut e ultramicrotome, by Leica (1 mentions) Ultrascan 4000 ccd camera, by Ametek (1 mentions) First light digital camera controller, by Ametek (1 mentions) Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) Agno3, by Merck Group (1 mentions) Agnps, by NanoComposix (1 mentions) Agnps, by US Research Nanomaterials (1 mentions) Uranyl acetate, by Ted Pella (1 mentions) C4742 95 digital camera, by Hamamatsu Photonics (1 mentions) Orius sc1000b, by Ametek (1 mentions) Quanta 450 feg microscope, by Thermo Fisher Scientific (1 mentions) Poly bed 812 dmp30, by Polysciences (1 mentions) Ultramicrotome, by Reichert Technologies (1 mentions)

Close spelling variants of this product

Transmission electron microscope (202 citations) JEM-1200 (86 citations) JEM-1200 EXII electron microscope (48 citations) JEM-1200 electron microscope (28 citations) JEOL JEM 1200 EXII (19 citations) JEOL 1200 electron microscope (19 citations) JEM-1200 transmission electron microscope (15 citations) 1200 EXII transmission electron microscope (5 citations) JEOL 1200 EXII microscope (5 citations) JEM-1200 EXII Scanning Transmission Electron Microscope (3 citations)

18 protocols using jem 1200 exii transmission electron microscope

Ultrastructural Analysis of Autophagy in HT29 Cells

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HT29 cells in the wells were fixed in 2% glutaraldehyde (0.1 M Millonig buffer, pH 7.4) for 60 min. After washing with both 0.1 M of phosphate buffer (3 times for 5 min) and 0.1 M pH 7.2 sodium cacodylate buffer (3 times for 5 min), the samples were post-fixed with 1% osmium tetroxide in 0.1 M sodium-cacodylate buffer for 60 min at 4°C in the dark. After washing 3 times for 5 min with sodium-cacodylate buffer (pH 7.4), the cells were pelleted by centrifugation and embedded in 10% gelatin in phosphate buffer (pH 7.4). After dehydration in a graded series of alcohol, the samples were embedded into Poly/Bed epoxy resin. Ultrathin sections (70–80 nm) were contrast stained with uranyl acetate and lead citrate, respectively. Ultrastructural analyses were performed by using a JEM-1200EXII Transmission Electron Microscope (JEOL, Akishima, Tokyo, Japan).
The average number of autophagic vacuoles was counted in five HT29 cells per sample (mean ± SD/cell).

Sipos F., Bohusné Barta B., Simon Á., Nagy L., Dankó T., Raffay R.E., Petővári G., Zsiros V., Wichmann B., Sebestyén A, & Műzes G. (2022). Survival of HT29 Cancer Cells Is Affected by IGF1R Inhibition via Modulation of Self-DNA-Triggered TLR9 Signaling and the Autophagy Response. Pathology and Oncology Research, 28, 1610322.

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Ultrastructural Analysis of Rabbit Brain and Kidney

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Brain and kidney specimens from the seven rabbits were fixed in 2.5% glutaraldehyde, kept for 4 h at 4 °C, thoroughly rinsed overnight at 4 °C in 0.1 M phosphate buffer, fixed in 1% osmium tetraoxide (OsO₄) for 30 min at 4 °C, then washed in PBS (pH 7.3) for 1 h followed by dehydration in an ethanol gradient (50–90% for 15 min, 100% for 1 h). The samples were then routinely processed and embedded in Epon-Araldite. Semithin sections (0.5 µm) were cut with a glass knife using a microtome (RMC Inc., Tucson, AZ, USA) and collected on copper grids and then stained with 1% toluidine blue for 20 min, washed in distilled water and placed in Permount. The obtained slides were examined and photographed using an Olympus DP20 microscope (Olympus, Tokyo, Japan) [14 (link)]. Ultrathin sections were stained for 15 min with a saturated uranyl acetate solution and counterstained in lead citrate for 20 min (thickness 500–700 Angstrom). Grids containing ultrathin sections of the material were analysed and photographed using a JEM-1200 EX II transmission electron microscope (Jeol, Tokyo, Japan). This was performed at the Electron Microscope Unit at the Military Veterinary Hospital in Nasr City, Cairo, Egypt.

Morsy E.A., Salem H.M., Khattab M.S., Hamza D.A, & Abuowarda M.M. (2020). Encephalitozoon cuniculi infection in farmed rabbits in Egypt. Acta Veterinaria Scandinavica, 62, 11.

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Ultrastructural Analysis of Pollinated Stigmas

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Pollinated stigmas were dry‐fixed using the method of Elleman & Dickinson (1986). Samples were washed with 0.1 M sodium cacodylate buffer pH 7.4 and prepared for scanning electron microscopy (SEM) and transmission electron microscopy (TEM) by a modified method of Villar et al. (1987) using 2.5% glutaraldehyde and low viscosity resin. Samples for SEM were gold‐coated and imaged using a Jeol JSM640LV Scanning Electron Microscope (Jeol, Tokyo Japan). Samples for TEM were ultra‐thin sectioned (100 nm) on an Ultracut‐E ultramicrotome (Leica, London, UK) and imaged by Jeol JEM1200EXII transmission electron microscope.

Wang L., Clarke L.A., Eason R.J., Parker C.C., Qi B., Scott R.J, & Doughty J. (2016). PCP‐B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen–stigma interactions. The New Phytologist, 213(2), 764-777.

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Ultrastructural Analysis of Gametocytes

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Gametocytes 12 days after induction from the WT 3D7 and Δpfdozi lines were purified by centrifugation on a 35% and 50% Percoll step gradient. The cells were washed with the RPMI-1640 medium and fixed by resuspension in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2) containing 2 mM CaCl₂. Cells were then immediately centrifuged at 1700 g for 10 min to collect the pellet. Fresh fixative was added to resuspend the pellet and incubated at room temperature for at least 1 h. Then, they were fixed in 2% osmium tetroxide for 1.5 h and en bloc stained in 2% aqueous uranyl acetate for 1 h. The cells were embedded in Eponite 12 (Ted Pella, CA). Ultrathin sections were prepared, contrasted with uranyl acetate and lead citrate, and examined with a JEOL JEM1200 EXII transmission electron microscope (Peabody, MA).

Min H., Liang X., Wang C., Qin J., Boonhok R., Muneer A., Brashear A.M., Li X., Minns A.M., Adapa S.R., Jiang R.H., Ning G., Cao Y., Lindner S.E., Miao J, & Cui L. (2024). The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum. Nature Communications, 15, 3747.

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Electron Microscopy of Liver Tissue

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EM analyses were performed at the High Resolution Electron Microscopy Facility of Medical College of Georgia at Augusta University. Livers were in situ fixed by perfusion with 2% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, postfixed with 2% mOsO₄ (also in sodium cacodylate buffer) for 1 h. After dehydration, tissues were embedded in epon-Araldite resin. 70-nM thin sections were cut on a MT-7000 ultramicrotome and collected on copper grids. Sections were stained with uranyl acetate and lead citrate and examined under a JEM-1200 EXII transmission electron microscope (JEOL USA Inc.). Images were captured with an UltraScan 4000 CCD camera and First Light Digital Camera Controller (Gatan Inc.).

Qiao A., Jin X., Pang J., Moskophidis D, & Mivechi N.F. (2017). The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. The Journal of Cell Biology, 216(3), 723-741.

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Negative Staining of Extracellular Vesicles

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EV pellets, isolated as described above, were resuspended in 4% electron microscopy grade paraformaldehyde in sodium phosphate buffer (Electron Microscopy Sciences, Hatfield, PA). 10 μL of the EVs were placed on Formvar-carbon coated electron microscopy grids (Electron Microscopy Sciences, Hatfield, PA) for 20 minutes, after which excess solution was wicked off with a filter paper. Grids were negatively stained with a uranyl-oxalate solution consisting of a 1:1 solution of 4% uranyl acetate and 0.15M oxalic acid, adjusted to pH 7 with ammonium hydroxide (all reagents from Electron Microscopy Sciences, Hatfield, MA) for 5 minutes. Excess stain was removed using a filter paper and allowed to dry. Grids were examined with a JEOL JEM-1200 EXII transmission electron microscope (Tokyo, Japan). Images were captured with an ORCA-HR digital camera (Hamamatsu, Hamamatsu, Japan) and recorded with an AMT Image Capture Engine (Advanced Microscopy Techniques, Woburn, MA).

Liu B., Lee B.W., Nakanishi K., Villasante A., Williamson R., Metz J., Kim J., Kanai M., Bi L., Brown K., Di Paolo G., Homma S., Sims P.A., Topkara V.K, & Vunjak-Novakovic G. (2018). Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells. Nature biomedical engineering, 2(5), 293-303.

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Ultrastructural Analysis of G361 Cells

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G361 cells were grown on Aclar film coated with collagen. After treatments as indicated, the G361 cells were fixed at 4 °C for 1.5 h with a fixative of 4 % PFA and 2.5 % glutaraldehyde in 0.1 M phosphate buffer (pH 7.4). Cells were then rinsed with phosphate buffer and after fixation in 1 % osmium tetroxide, the cells were put through a graded ethanol series. Cells were removed from the Aclar film and placed in a glass scintillation vial containing propylene oxide (PO). Dehydration was performed with three sequential incubations, each for 5 min in fresh PO, and one final incubation in fresh PO for 10 min. Cells were infiltrated with a mixture of 1:1 Epon:PO for 1 h, then with a 2:1 mixture for 2 h, then with pure Epon for 2 h, and finally with Epon and an added accelerator for 42-48 h. Cells were embedded in Epon, and ultrathin sections were examined using a JEM 1200EX-II transmission electron microscope (Jeol; Tokyo, Japan).

Choi B.B., Choi J.H., Hong J.W., Song K.W., Lee H.J., Kim U.K, & Kim G.C. (2017). Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. International Journal of Medical Sciences, 14(11), 1101-1109.

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Comprehensive Characterization of Silver Nanoparticles

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AgNPs, 10 nm in seize: capped with LA, PEG and TA, water dispersed were obtained from Nanocomposix Europe; AgNPs 10 nm: uncapped, water dispersed - US Research Nanomaterials (Houston, TX, USA). AgNO₃ was obtained from Sigma-Aldrich (Poland).
Characterization of AgNPs was performed by the manufacturer, according to good laboratory practice 30 . The size of AgNPs was measured using JEOL 1010 transmission electron microscope (TEM), mass concentration - Thermo Fisher X Series 2 ICP-MS, spectral properties - Agilent 8453 UV-Visible Spectrometer, zeta potential and hydrodynamic diameter - Malvern Zetasizer nano ZS. Measurement of AgNPs-UC size and size distribution was performed by JEM 1200 EXII transmission electron microscope (JEOL, Japan) at an operational voltage of 200 kV. For TEM measurements, a drop of the solution of AgNPs was placed on a carbon-coated copper grid and allowed to dry to record TEM images. Particle size distribution was obtained from a histogram considering more than 300 particles measured using multiple TEM micrographs. Additionally, measurements of zeta potential and hydrodynamic diameter by Malvern Zetasizer nano ZS (Malvern Instruments, Malvern, UK) were taken six times for all tested AgNPs at concentration 20 μg/mL in serum-free (SF) culture medium at room temperature.

Niska K., Knap N., Kędzia A., Jaskiewicz M., Kamysz W, & Inkielewicz-Stepniak I. (2016). Capping Agent-Dependent Toxicity and Antimicrobial Activity of Silver Nanoparticles: An In Vitro Study. Concerns about Potential Application in Dental Practice. International Journal of Medical Sciences, 13(10), 772-782.

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Ultrastructural Analysis of Autophagic Vacuoles in HT29 Cells

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For 60 minutes, HT29 cells in the wells were fixed in 2% glutaraldehyde (0.1M Millonig buffer, pH 7.4). Following three 5-minute washes with 0.1 M phosphate buffer and 0.1 M pH 7.2 sodium cacodylate buffer, the samples were post-fixed for 60 minutes at 4°C in the dark with 1% osmium tetroxide in 0.1 M sodium-cacodylate buffer. After three 5-minute washes with sodium-cacodylate buffer (pH 7.4), cells were centrifuged and embedded in 10% gelatin in phosphate buffer (pH 7.4). Following dehydration in progressively increasing concentrations of alcohol, the samples were embedded in Poly/Bed epoxy resin. Contrast staining of ultrathin sections (70–80 nm) with uranyl acetate and lead citrate, respectively. JEM-1200EXII Transmission Electron Microscope was used to conduct ultrastructural analyses (JEOL, Akishima, Tokyo, Japan).
In five HT29 cells per sample, the average number of autophagic vacuoles was counted (mean ± SD/cell).

Bohusné Barta B., Simon Á., Nagy L., Dankó T., Raffay R.E., Petővári G., Zsiros V., Sebestyén A., Sipos F, & Műzes G. (2022). Survival of HT29 cancer cells is influenced by hepatocyte growth factor receptor inhibition through modulation of self-DNA-triggered TLR9-dependent autophagy response. PLoS ONE, 17(5), e0268217.

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TEM Imaging of CGφ29 Phage

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CGφ29 was imaged using a JEM–1200 EXII Transmission Electron Microscope (JEOL, Tokyo, Japan) and a C4742-95 digital camera (Hamamatsu). Five μL of phage suspension was adsorbed to a 400 mesh Formvar copper film grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min. The grid was rinsed with sterile Nanopure water for 15 s and stained with 1% w/v uranyl acetate (Ted Pella, Redding, CA, USA) for 2 min, air dried, and imaged.

Rodela M.L., Sabet S., Peterson A, & Dillon J.G. (2019). Broad Environmental Tolerance for a Salicola Host-Phage Pair Isolated from the Cargill Solar Saltworks, Newark, CA, USA. Microorganisms, 7(4), 106.

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