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Sodium meta arsenite naaso2

Manufactured by Merck Group
Sourced in United States

Sodium meta-arsenite (NaAsO2) is a chemical compound used in various industrial and laboratory applications. It is a white or colorless crystalline solid with a chemical formula of NaAsO2. Sodium meta-arsenite is a source of the arsenite ion (AsO2-) and is commonly used as a reagent in analytical chemistry, as well as in the production of other chemical compounds.

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7 protocols using sodium meta arsenite naaso2

1

Functionalization of Silk Fabric with Sodium Meta-Arsenite

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Sodium meta-arsenite NaAsO2 was obtained from Sigma-Aldrich, while HCl, NaOH, KNO3, and Cu (NO3) ∙ 3H2O obtained from Merck. 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid (call MES hydrate) & 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)(call HEPES), used for pH buffering, were obtained from Sigma-Aldrich. Milli-Q Academic system, Millipore produced ultrapure water.
The Silk Fabric (SF) provided by Tsiakiris Georgios Silk Company, Alexandroupoli, Greece. Sodium carbonate (Na2CO3) purchased from Riedel de Haën. The coupling agent 3-(chloropropyl)trimethoxysilane was provided by Fluka. Methanol and ethanol purchased from Merck and diethyl ether from Sigma Aldrich.
All reagents were of analytical reagent grade purity, and all solutions prepared using deionized water obtained with a Milli-Q system with a conductivity of 18.2 μS cm−1.
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2

Cellular Assays for Misfolded Protein

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A549 (lung carcinoma), SKOV3 (ovarian carcinoma), and U2OS (osteosarcoma) cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB) (Osaka, Japan). C6 (rat glioma) was obtained from the Cell Resource Center for Biomedical Research, Tohoku University, Sendai, Japan. All cells were cultured in DMEM (Dulbecco’s Modified Eagle’s medium) supplemented with 5% fetal bovine serum at 37 °C with 95% O2 and 5% CO2 in a humidified incubator. Lipofectamine 2000 (Invitrogen) in Opti-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) media was used for transfection. Hypoxia responsive cells were obtained by stable transfection of plasmid encoding luciferase reporter driven by HIF-1α promoter as described earlier [37 (link)]. For heat shock induced misfolding of protein, cells transfected with plasmid (pGL4) encoding luciferase driven by a constitutive promoter were used [38 (link)]. For protein aggregation assays, GFP protein was used as a model. Cells expressing mortalin-GFP protein were generated by stable transfections of pEGFP-C1/mot-GFP plasmid. Sodium(meta)arsenite (NaAsO2), used in an induction of protein aggregation, was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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3

CRISPR-Mediated ATF3 Knockout in BEAS-2B Cells

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Human bronchial epithelial BEAS-2B cells were purchased from the China Center for Type Culture Collection (CCTCC, Wuhan, China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, NY, USA). ATF3 KO BEAS-2B cells were custom developed by using a CRISPR/Cas9 gene-editing method by Cyagen Biosciences (Suzhou, China). Sodium meta-arsenite (NaAsO2) was purchased from Sigma (St Louis, MO, USA). The inhibitors, SB203580, SP600125, U0126 and LY294002, were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against ATF3 (33593), DR5 (3696), JNK (9252), p-JNK (4668), p38 (8690), p-p38 (9215), Bcl-xL (2764), caspase 8 (9746), cleaved caspase 8 (8592), caspase 3 (14220), cleaved caspase 3 (9664), caspase 9 (9502), cleaved caspase 9 (9509), α-tubulin (2125), AKT (C67E7), p-AKT (4060T), ERK (4695), p-ERK (91015) and β-actin (4970) were purchased from Cell Signaling Technologies (Danvers, MA, USA).
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4

Waste Fe/Mn Oxides Characterization

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The waste Fe/Mn oxides (Mn-WTRs) from water deironing were collected from “Na Grobli” Water Treatment Plant, Wrocław, Poland. The detailed characterization of this by-product was presented elsewhere (Ociński et al. 2016b (link)). The raw sludge was rinsed several times with distilled water, pre-treated with 0.1 M HCl, dried, ground, and sieved (0.25-mm sieve). All reagents used in this study were of analytical grade. Medium molecular weight chitosan, sodium (meta)arsenite (NaAsO2), and disodium hydrogen arsenate (Na2HAsO4·7H2O) were obtained from Sigma-Aldrich; glutaraldehyde solution (25% w/w) used as the cross-linking agent was acquired from Chempur, Poland.
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5

Optimized Camellia sinensis Extraction

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The raw matured leaves (without any preservatives, pesticides or contaminants) of Camellia sinensis were collected locally and dried/dehydrated in an incubator for several days at 37 °C. Those were grinded in moisture free condition to make dust. Extraction was done by taking the freshly prepared Camellia sinensis leaf dust of 1 g% in distilled water. To achieve the optimum extraction of the bioactive phytochemicals, antioxidants and flavonoids, catechin and theaflavin products higher temperature (100 °C) should be avoided. Longer duration of extractions (∼24 h) may also yield fewer amounts of bioactive compounds (Banerjee and Chatterjee, 2015 (link)). In the current study, 4–5 h of extraction process was continued at 70 °C. When the color of the aqueous extract became dark brown, it was collected for the experiment and stored after lyophilization. This condition was termed as optimum and it was reported earlier (Acharyya et al., 2014 (link)). The total antioxidant capacity of the extract solution was found to be the maximum in the present set up of the experiment. Sodium-meta-arsenite (NaAsO2) from Sigma-Aldrich (St Louis, MO, USA), Chemicals was dissolved in distilled water to prepare 0.6 ppm final concentration. We used this dose because it was not lethal for the animal but generated tissue toxicity after a moderate time of its exposure (Acharyya et al., 2014 (link)).
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6

Characterizing Crosslinked CNF Hydrogels

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CNFs were provided by the Process Development Center (PDC) of the University of Maine (Orono, ME, USA). The 3 wt.% CNFs were produced by mechanically refining bleached softwood Kraft pulp. This is the standard grade of CNFs produced by the PDC and contains 90% fines. In this context, the fines content indicates the percentage of fibers with lengths < 200 µm. PolycupTM (polyamide-epichlorohydrin) 5150 crosslinker (26 wt.%) was supplied by Solenis (Wilmington, DE, USA). Anhydrous ferric chloride (FeCl3; 98%) and magnesium chloride (MgCl2; 99%) were purchased from Alfa Aeser (Haverhill, MA, USA). Anhydrous ethyl alcohol (C2H5OH; 99.5%) was obtained from Acros Organics (Jair Lawn, NJ, USA). HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffering agent (≥99.5%), sodium chloride (NaCl; ≥99%), sodium hydroxide (NaOH; ≥97%), hydrochloric acid (HCl; 37%), sodium (meta) arsenite (NaAsO2), and sodium arsenate dibasic heptahydrate (Na2HAsO4.7H2O; ≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals and solvents were used without any further purification.
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7

Biochemical Markers in Cadmium Toxicity

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Cadmium chloride (CdCl 2• 2½H 2 O) was purchased from Mallinckrodt Chemical Works (St. Louis, MO, USA). Sodium (meta) arsenite (NaAsO 2 ) was purchased from Sigma-Aldrich. Protease inhibitor cocktail, ACTB (β-actin), soluble guanylyl cyclase α1 (GUCY1A3), and β1 (GUCY1B3) antibodies were obtained from Sigma-Aldrich. Ketamine and xylazine were purchased from König (Buenos Aires, Argentina). Ketofen was obtained from Vetanco S.A. (Buenos Aires, Argentina). Proliferating cell nuclear antigen (PCNA), cyclin D3 (CCND3), and ERα antibodies were purchased from Santa Cruz Biotechnology. Anti-recombinant rat PRL and anti-rLH-11 antisera were provided by Dr A F Parlow (National Hormone and Pituitary Program, Torrance, CA, USA).
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