Dextran sulfate sodium salt, weight-average molecular weight (MW) 500 kDa (#BP1585–100), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, #22981), Gibco™ HEPES 1M (pH 7.2–7.5, #15630–080), and Invitrogen™ UltraPure™ DNase/RNase-Free Distilled water were purchased from Thermo Fisher Scientific. Chitosan (MW range 50–190 kDa, 75%–85% deacetylated) (#448869), zinc sulfate (#204986), mannitol (#M9546), succinic acid (#S3674), N-hydroxysuccinimide (NHS, #130672), Dulbecco’s phosphate-buffered saline (PBS) and 10x DPBS (# D8537 and #59331C), and lysozyme from chicken egg white (#L2879) were obtained from Sigma-Aldrich. Recombinant human stromal cell-derived factor-1α, SDF-1α (SDF), and human vascular endothelial growth factor165, VEGF165 (VEGF), were prepared in the authors’ laboratory as previously described.(Lauten et al., 2010 (link); Yin et al., 2013 (link)) Human CXCL12/SDF-1 DuoSet ELISA kit (#DY350) was purchased from R&D Systems.
Human cxcl12 sdf 1 duoset elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human CXCL12/SDF-1 DuoSet ELISA kit is a set of reagents designed for the quantitative determination of human CXCL12/SDF-1 levels in cell culture supernates, serum, and plasma samples.
Automatically generated - may contain errors
Lab products found in correlation
Lysozyme from chicken egg white, by Merck Group (1 mentions)
Succinic acid, by Merck Group (1 mentions)
Mannitol, by Merck Group (1 mentions)
Ultrapure dnase rnase free distilled water, by Thermo Fisher Scientific (1 mentions)
Chitosan, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Zinc sulfate, by Merck Group (1 mentions)
Infinite m200 pro microplate reader, by Tecan (1 mentions)
2 protocols using human cxcl12 sdf 1 duoset elisa kit
1
Dextran Sulfate Hydrogel for Cell Delivery
2
Quantification of Secreted CXCL12 by ELISA
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The secreted cytokine CXCL12 was quantified by a human CXCL12/SDF-1 DuoSet ® ELISA kit (R&D Systems, Minneapolis, USA). Quantification was performed according to the manufacturer's protocol, as previously described (Habauzit et al., 2010) (link). The results of ELISA were assessed by measuring the absorbance at 450 and 570 nm with the Infinite M200 PRO microplate reader (Tecan, Switzerland).
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