Ammonium iron 2 sulfate hexahydrate

Single-stranded M13mp18 viral DNA, T4-βGT (10 units/μl), MspI (20 units/μL) and uridine-diphosphoglucose (UDP-Glc) were purchased from New England Biolabs (Ipswich, MA, USA). TET1 was purchased from Wisegene (Chicago, IL, USA). ARP [aldehyde reactive probe; O-(biotinylcarbazoylmethyl) hydroxylamine] was purchased from Cayman Chemical (Ann Arbor, MI, USA). Recombinant human TDG protein was purchased from Novus Biologicals (Centennial, CO, USA). Real-time PCR mix was purchased from Takara (Kyoto, Japan). All short oligonucleotides including staple DNAs were purchased from Eurofins Genomics (Tokyo Japan). Chemically modified DNA oligonucleotides were obtained from Japan Bioservices (Saitama, Japan). Ammonium iron (II) sulfate hexahydrate, l-ascorbic acid, α-ketoglutarate, dl-dithiothreitol, adenosine triphosphate, 25% glutaraldehyde acid were purchased from Wako pure chemical industries (Kyoto, Japan). 1 M HEPES buffer (pH 7.5), 1 M Tris buffer (pH 7.6) were obtained from Sigma-Aldrich. The buffer 10× NEB 4 [500 mM potassium acetate, 200 mM Tris-acetate, 100 mM magnesium acetate, 10 mM DTT], and 10× cut smart buffer [500 mM potassium acetate, 200 mM Tris-acetate, 100 mM magnesium acetate, 1 mg/ml BSA] were provided by New England Biolabs.