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Electro chemiluminescence immunoassay

Manufactured by Beckman Coulter
Sourced in Japan

Electro-chemiluminescence immunoassay is a laboratory technique that utilizes electrochemical and luminescent principles to detect and quantify specific analytes or targets in a sample. The core function of this method is to generate a measurable luminescent signal that is proportional to the concentration of the target molecule in the sample.

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3 protocols using electro chemiluminescence immunoassay

1

Midluteal Uterine Artery Blood Flow Evaluation

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An ultrasound examination of uterine artery blood flow was performed using a 2.9-MHz (range, 2–5 MHz) transvaginal microconvex probe (GE Voluson E8; GE Healthcare Austria GmbH & Co OG, Zipf, Austria). The examiners were blinded to the history of all subjects.
Ultrasonic Doppler assessment was performed during the midluteal phase at 7 to 10 days after women’s ovulation, which was confirmed by follicular monitoring through an ultrasound examination. Serum progesterone levels were measured during the midluteal phase with an electrochemiluminescence immunoassay (Beckman Coulter, Tokyo, Japan).
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2

Thyroid Hormone Measurement Protocol

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TSH was determined by chemiluminescence immunoassay (provided by Beckman Coulter Inc., Brea, California) with a functional sensitivity of 0.015 mIU/L. Tg and TgAb levels were determined using electro-chemiluminescence immunoassay (provided by Beckman Coulter Inc., Brea, California) with a measuring range of 0.100–500 ng/mL and 0.900–2500 IU/mL, respectively.
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3

Serum Biomarkers and Immunoglobulin Measurement

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Serum levels of IL-10 (Bender MedSystems, Labclinics, Madrid, Spain) and sFASL (R&D, Vitro, Madrid, Spain) were measured in duplicate by enzyme-linked immunosorbent assay. Vitamin B12 were measured by electro-chemiluminescence immunoassay (Beckman Coulter). Serum immunoglobulin concentrations were determined by nephelometry (Beckman Coulter) (14 (link)).
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