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Anti suz12

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Anti-SUZ12 is a laboratory reagent used for research purposes. It is an antibody that specifically binds to the SUZ12 protein, which is a component of the Polycomb Repressive Complex 2 (PRC2) involved in epigenetic gene regulation.

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Lab products found in correlation

Anti bmi 1, by Merck Group (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) Anti mel18, by Abcam (1 mentions) Anti cbx4, by Santa Cruz Biotechnology (1 mentions) Superscript 3 first strand synthesis supermix, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Anti v5, by Thermo Fisher Scientific (1 mentions) Anti ezh2, by Merck Group (1 mentions) Streptavidin hrp, by Thermo Fisher Scientific (1 mentions) Anti h2aub1, by Cell Signaling Technology (1 mentions) Anti brachyury, by Santa Cruz Biotechnology (1 mentions) Nanog, by Merck Group (1 mentions) Sall4, by Abcam (1 mentions) Gapdh hrp, by Santa Cruz Biotechnology (1 mentions) H3k4me3, by Active Motif (1 mentions) H3k27me3, by Merck Group (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Anti mettl3, by Abcam (1 mentions) Anti nanog, by Abcam (1 mentions) Anti pim1, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti suz12

1

Investigating EBNA3 Protein Interactions

Cited 5 times
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Western blots and immunoprecipitations were performed as described previously (12 (link)). Antibodies for western blot were anti-EBNA3A (Abcam, ab16126, 1:1000 dilution), anti-EBNA3B (clone 6C9, Allday lab, E. Kremmer (39 (link)), 1:10 dilution), anti-EBNA3C (clone A10, gift from M. Rowe, University of Birmingham, 1:10 dilution), anti-γ-tubulin (Sigma, T6557, 1:8000 dilution), anti-BMI1 (Millipore, 05–637, 1:1000 dilution), anti-SUZ12 (Santa Cruz Biotechnology, sc-46264, 1:1000 dilution), anti-CBX4 (Santa Cruz, sc-517216, 1:1000 dilution) and anti-MEL18 (Abcam, ab5267, 1:1000 dilution). Antibodies for immunoprecipitations were anti-BMI1 (Bethyl Laboratories, A301-694A, 2 μg) and DYKDDDDK Tag antibody (NEB, 2368, 2 μg). RNA extraction was performed using Qiagen’s RNeasy mini kit, according to the manufacturer’s instructions. cDNA was obtained using Invitrogen’s SuperScript III First Strand Synthesis Supermix. QPCR for cDNA and DNA from ChIP was performed using Platinum SYBR green QPCR Supermix uracil DNA glycosylase (UDG) kit (Invitrogen), as described previously (12 (link)). Primers for STK39 (8 (link)), AICDA (40 (link)) and COBLL1 (10 (link)) loci and expression have been described before. All the oligonucleotide primers used are listed in Supplementary Table S1.
Paschos K., Bazot Q., Lees J., Farrell P.J, & Allday M.J. (2019). Requirement for PRC1 subunit BMI1 in host gene activation by Epstein–Barr virus protein EBNA3C. Nucleic Acids Research, 47(6), 2807-2821.
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2

Pluripotency Protein Analysis Protocol

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Smc3 (Abcam, Ab9263), Sall4 (Abcam, Ab29112), Wapal (Abcam, Ab70741), Scc1/Rad21 (Abcam, Ab992), Nanog (Millipore, AB5731), GAPDH-HRP (Santa Cruz, sc-25778), total H3 (Active Motif, 61278), H3K27me3 (Millipore, 07-449), H3K4me3 (Active Motif, 39916), anti-Brachyury (Santa Cruz, sc-17743), anti-v5 (Invitrogen, 46-0708), Streptavidin–HRP (Invitrogen, SA1007), anti-Suz12 (Santa Cruz, sc-46264), anti-Ezh2 (Millipore, 17-662), anti-Ring1b (Western Blot-Active Motif, 39664; ChIP-seq Abcam, Ab101273), anti-H2Aub1 (Cell Signaling, 8240).
Stelloh C., Reimer M.H., Pulakanti K., Blinka S., Peterson J., Pinello L., Jia S., Roumiantsev S., Hessner M.J., Milanovich S., Yuan G.C, & Rao S. (2016). The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics & Chromatin, 9, 14.
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3

Western Blot Analysis of Epigenetic Markers

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Pathological and Western blot analyses were performed as previously described38 (link). Antibodies used for Western blot included anti-EED, anti-EZH2, and anti-monomethyl-Histone H3 (Lys27) (Merck Millipore, Billerica, MA, USA); anti-dimethyl-Histone H3 (Lys27), anti-trimethyl-Histone H3 (Lys27), and anti-histone H3 (Cell Signaling, Danvers, MA, USA); and anti-SUZ12 (SantaCruz Biotechnology, Santa Cruz, CA, USA).
Ikeda K., Ueda T., Yamasaki N., Nakata Y., Sera Y., Nagamachi A., Miyama T., Kobayashi H., Takubo K., Kanai A., Oda H., Wolff L., Honda Z.I., Ichinohe T., Matsubara A., Suda T., Inaba T, & Honda H. (2016). Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency. Scientific Reports, 6, 29454.
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4

Western Blot Analysis of Protein Expression

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Samples were separated on a 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto nitrocellulose membranes and then blocked in 10% dry milk‐TBST (20 mmol/L Tris‐HCl [PH 7.6], 127 mmol/L NaCl, 0.1% Tween‐20) for two hours at 37°C. Following three washes in Tris‐HCl pH 7.5 with 0.1% Tween 20, the blots were incubated with primary antibody overnight at 4°C. Following three washes, membranes were then incubated with secondary antibody overnight at 4°C. Primary antibodies include the following: anti‐glypican‐3 (Abcam), anti‐PCNA (Abcam), anti‐METTL3 (Abcam), anti‐histone H3 (Abcam), anti‐H3K9me3 (Abcam), anti‐JMJD2A (Santa Cruz, Biotech), anti‐Pim1 (Santa Cruz, Biotech), pHistone H3 (Abcam), anti‐SUZ12 (Santa Cruz, Biotech), anti‐SUV39H1 (Santa Cruz, Biotech), anti‐Nanog (Abcam), anti‐MEKK4 (Abcam), anti‐pTyr (Abcam). Signals were visualized by enhanced chemiluminescence plus kit (GE Healthcare) according to our pervious protocol.34
Yang Y., Song S., Meng Q., Wang L., Li X., Xie S., Chen Y., Jiang X., Wang C., Lu Y., Xin X., Pu H., Gui X., Li T., Xu J., Li J., Jia S, & Lu D. (2020). miR24‐2 accelerates progression of liver cancer cells by activating Pim1 through tri‐methylation of Histone H3 on the ninth lysine. Journal of Cellular and Molecular Medicine, 24(5), 2772-2790.
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