Automated syringe pump

Manufactured by Stoelting

Sourced in United States

The Automated Syringe Pump is a precision instrument designed to accurately dispense and withdraw fluids. It utilizes a stepper motor to control the linear movement of a syringe plunger, enabling precise and reproducible fluid delivery. The device can be programmed to deliver a wide range of flow rates and volumes, making it a versatile tool for various laboratory applications.

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Lab products found in correlation

Norepinephrine, by Merck Group (2 mentions) Propranolol hydrochloride, by Merck Group (1 mentions) Propranolol, by Merck Group (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Hamilton syringe, by Hamilton Company (1 mentions) Xylazine, by Akorn (1 mentions) Ketamine, by Vedco (1 mentions)

6 protocols using automated syringe pump

Bilateral Amygdalar and Insular Modulation

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NE [(±)-1-(3,4-dihydroxyphenyl)-2-aminoethanol hydrochloride; Sigma-Aldrich] was dissolved in sterile saline and administered bilaterally into the BLA (1.0 μg in 0.2 μL), aIC (2.5 μg in 0.5 μL), or pIC (2.5 μg in 0.5 μL) immediately after the training trial (or to HC control rats) (5 (link)). The β-adrenoceptor antagonist propranolol hydrochloride (Sigma-Aldrich) was also dissolved in saline and administered bilaterally into the BLA (0.3 μg in 0.2 μL), aIC (0.75 μg in 0.5 μL), or pIC (0.75 μg in 0.5 μL) immediately after training (5 (link)). The GABAergic agonist muscimol (0.07 μg in 0.5 μL, 3-hydroxy-5-aminomethyl-isoxazole, Research Biochemicals International) was dissolved in saline and administered bilaterally into the aIC 1 h after the training trial (57 (link)). Drug infusions were given via 30-gauge injection needles connected to 10-μL Hamilton microsyringes by polyethylene (PE-20) tubing. The injection needles protruded 2.0 mm beyond the cannula tips, and drug or an equivalent volume of saline control, at a rate of 0.4 μL/min, was infused by an automated syringe pump (Stoelting Co.). The injection needles were retained within the cannulas for an additional 20 s. All drug solutions were freshly prepared before each experiment.

Chen Y.F., Song Q., Colucci P., Maltese F., Siller-Pérez C., Prins K., McGaugh J.L., Hermans E.J., Campolongo P., Kasri N.N, & Roozendaal B. (2022). Basolateral amygdala activation enhances object recognition memory by inhibiting anterior insular cortex activity. Proceedings of the National Academy of Sciences of the United States of America, 119(22), e2203680119.

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Norepinephrine and Propranolol Modulate Memory Consolidation

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Norepinephrine (0.3, 1.0, or 3.0 μ g; Sigma-Aldrich) or the β-adrenoceptor antagonist propranolol (0.1, 0.3, or 1.0 μ g; Sigma-Aldrich) was dissolved in saline and administered into the BLA immediately after the training phase. Bilateral infusions of drug or an equivalent volume of saline were given via 30-gauge injection needles connected to 10-μ l Hamilton microsyringes by polyethylene (PE-20) tubing. The injection needles protruded 2.0 mm beyond the cannula tips and a 0.2-μ l injection volume per hemisphere was infused over a period of 30 s by an automated syringe pump (Stoelting). The injection needles were retained within the cannulae for an additional 20 s to maximize diffusion and to prevent backflow of drug into the cannulae. The infusion volume was based on previous findings from our laboratory indicating that similar infusions into the adjacent central amygdala do not affect memory consolidation (Roozendaal and McGaugh, 1996 (link), 1997 (link)). All drug solutions were freshly prepared before each experiment.

Barsegyan A., McGaugh J.L, & Roozendaal B. (2014). Noradrenergic activation of the basolateral amygdala modulates the consolidation of object-in-context recognition memory. Frontiers in Behavioral Neuroscience, 8, 160.

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Norepinephrine Infusion in Basolateral Amygdala

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Norepinephrine (1.0 μg; Sigma-Aldrich) was dissolved in saline and administered into the BLA immediately after the object recognition training trial (Roozendaal et al., 2008 (link)). Bilateral infusions of drug or an equivalent volume of saline were administered into the BLA via 30-gauge injection needles connected to 10-μl Hamilton microsyringes by polyethylene (PE-20) tubing. The injection needles protruded 2.0 mm beyond the cannula tips and a 0.2-μl injection volume per hemisphere was infused over a period of 30 s by an automated syringe pump (Stoelting Co., Dublin, Ireland). The injection needles were retained within the cannulae for an additional 20 s to maximize diffusion and to prevent backflow of drug into the cannulae. The infusion volume was based on previous findings from our laboratory indicating that drug infusions into the adjacent central amygdala do not affect memory consolidation (Roozendaal and McGaugh, 1996 (link), 1997 (link)). Drug solutions were freshly prepared before each experiment.

Beldjoud H., Barsegyan A, & Roozendaal B. (2015). Noradrenergic activation of the basolateral amygdala enhances object recognition memory and induces chromatin remodeling in the insular cortex. Frontiers in Behavioral Neuroscience, 9, 108.

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Orthotopic Murine Glioblastoma Model

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Animal experiments were conducted in accordance with approved protocols by the Johns Hopkins University institutional animal care and use committee. All animals were housed at Johns Hopkins University animal facilities with free access to food and water.
The GL261 syngeneic, orthotopic, immunocompetent brain tumor model in C57BL/6 mice was chosen because it is well-established for effectively evaluating immunotherapies ^{45 (link)} Male and female C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME, USA) 6-8 weeks in age were intracranially inoculated with GL261 murine glioblastoma cells. Mice were anesthetized with a ketamine xylazine anesthesia cocktail. A burr hole was drilled 1 mm posterior and 2 mm lateral to the midline to inject cells into the striatum. 2 μL of GL261 cell solution (100,000 cells total) was injected into the striatum at a depth of 2.5 mm by a Hamilton syringe (Hamilton Company, Reno, NV, USA) using a stereotactic frame and automated syringe pump (Stoelting Co., Wood Dale, IL, USA). The syringe was withdrawn at a rate of 0.5 mm/min to reduce backflow, and the incision was sutured together.

Liaw K., Sharma R., Sharma A., Salazar S., La Rosa S.A, & Kannan R.M. (2020). Systemic Dendrimer Delivery of Triptolide to Tumor-Associated Macrophages Improves Anti-Tumor Efficacy and Reduces Systemic Toxicity in Glioblastoma. Journal of controlled release : official journal of the Controlled Release Society, 329, 434-444.

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Murine Glioblastoma Tumor Induction

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GL261 murine microglia were obtained from the DTP/DCTD/NCI Tumor Repository (National Cancer Institute, Frederick, MA, USA). Cells were cultured in RPMI supplemented with 10% FBS, 1% P/S, and 1% L-glutamine and maintained at 37°C and 5% CO₂ atmosphere. All animals were housed at Johns Hopkins University animal facilities and given free access to food and water. Experiments performed were approved by the Johns Hopkins Institutional Animal Care and Use Committee. Male and female C57BL/6 mice were obtained from Jackson Laboratory Company (Bar Harbor, ME, USA). Mice 6-8 weeks old were inoculated with glioblastoma tumors via intracranial injection of GL261 cells. Mice were anesthetized with a ketamine/xylazine cocktail for surgeries. An incision was created in the center of the scalp. A burr hole was drilled at 1 mm posterior to the bregma and 2 mm lateral to the midline for injection into the striatum in the right hemisphere. A 2 μL injection of 100,000 GL261 cells were injected to a depth of 2.5 mm over 10 min with a stereotactic frame and automated syringe pump (Stoelting Co., Wood Dale, IL, USA). The syringe was withdrawn, and the incision sutured (Ethicon Inc., Somerville, NJ, USA).

Sharma R., Liaw K., Sharma A., Jimenez A., Chang M., Salazar S., Amlani I., Kannan S, & Kannan R.M. (2021). Glycosylation of PAMAM Dendrimers Significantly Improves Tumor Macrophage Targeting and Specificity in Glioblastoma. Journal of controlled release : official journal of the Controlled Release Society, 337, 179-192.

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Murine Glioblastoma Intracranial Inoculation

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All animals were housed at Johns Hopkins University animal facilities and were given free access to food and water. Experiments performed were approved by the Johns Hopkins Institutional Animal Care and Use Committee (MO16M122).
Male and female C57BL/6 mice 6–8 weeks of age were inoculated intracranially with GL261 murine glioblastoma cells obtained from the DTP/DCTD/NCI Tumor Repository (National Cancer Institute, Frederick, Maryland). GL261 were maintained in RPMI with 10% FBS, 1% P/S, and 1% l‐glutamine at 37°C and 5% CO₂. Authentication and Mycoplasma testing were performed by the tumor repository. Cells were used within 2–3 passages after thawing. Mice were anesthetized with a ketamine (Vedco, St. Joseph, Missouri) and xylazine (Akorn Animal Health, Lake Forest, Illinois) cocktail. A midline scalp incision was made and a burr hole drilled 1 mm posterior to the bregma and 2 mm lateral to the midline. A 2 μl Hamilton syringe (Hamilton Company, Reno, Nevada) was lowered to a depth of 2.5 mm to inject 2 μl of GL261 solution containing 100,000 cells over 10 min using a stereotactic frame and automated syringe pump (Stoelting Co., Wood Dale, Illinois). The syringe was withdrawn at 0.5 mm/min, and the incision sutured.

Liaw K., Reddy R., Sharma A., Li J., Chang M., Sharma R., Salazar S., Kannan S, & Kannan R.M. (2020). Targeted systemic dendrimer delivery of CSF‐1R inhibitor to tumor‐associated macrophages improves outcomes in orthotopic glioblastoma. Bioengineering & Translational Medicine, 6(2), e10205.

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