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Lc 20at hplc instrument

Manufactured by Shimadzu
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Sourced in Japan

The Shimadzu LC-20AT HPLC instrument is a high-performance liquid chromatography system designed for analytical and preparative separation and purification of a wide range of chemical compounds. It features a dual-plunger, constant-flow pump that ensures precise and stable solvent delivery. The instrument is equipped with a comprehensive set of detectors, including a UV-VIS detector, to enable sensitive and selective analysis of samples.

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Jsm 7500f, by JEOL (1 mentions)

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LC-20AT (490 citations) HPLC instrument (30 citations) LC-20AT HPLC (28 citations) LC-20AT instrument (4 citations) 20AT HPLC instrument (2 citations)

3 protocols using lc 20at hplc instrument

1

Enzymatic Synthesis and HPLC Analysis of c-di-GMP

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Truncated tDgcA protein (10 μM) purified from BLtdgcA21 (link) was added into reaction mixtures containing 100 mM Tris-HCl (pH 8.0) and 10 mM MgCl2 in a 100 ml volume, and the reactions were started by addition of 0.1 mM GTP. After incubated at 37 °C for 24 h, the enzymatic reaction was stopped by heating to 95 °C for 5 min and then centrifuged at 12,000 rpm for 10 min to remove denatured proteins. The obtained supernatant was then loaded onto an Elite Hypersil BDS C18 Column (200 × 4.6 mm, 5 μm particle sizes) for monitoring the formation of c-di-GMP using a Shimadzu LC-20AT HPLC instrument (mobile phase: 90% 20 mM ammonium acetate (pH 6.8) and 10% methanol; flow rate: 1 ml min−1). Assays were performed in triplicate.
Su J., Zou X., Huang L., Bai T., Liu S., Yuan M., Chou S.H., He Y.W., Wang H, & He J. (2016). DgcA, a diguanylate cyclase from Xanthomonas oryzae pv. oryzae regulates bacterial pathogenicity on rice. Scientific Reports, 6, 25978.
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2

Analytical Characterization of PILs

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Fourier transform infrared spectra (FT-IR) were recorded by a Perkin Elmer Infrared spectrometer. The concentrations of the EGCG in the samples were determined by high-performance liquid chromatography (HPLC) using an LC-20AT HPLC instrument (SHIMADZU, Kyoto, Japan). The surface structure of the PILs was photographed by scanning electron microscope JSM-7500F (JEOL, Tokyo, Japan).
Luo Y., Zhang Y., Tao C., Ni H., Liu X., Chen Y., Wu Y., Song H, & Xu T. (2022). Exploring the Absorption Mechanisms of Imidazolium-Based Ionic Liquids to Epigallocatechin Gallate. International Journal of Molecular Sciences, 23(20), 12600.
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3

Quantitative Analysis of PCB Metabolites

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The HPLC-based separation and quantification of PCBs, OH-PCBs and PCB sulfates
was conducted using a modification of our previously published procedure (Grimm et al. 2013 (link)). Briefly, PCB metabolites were separated at a
flow rate of 1.5 ml/min on a C18 AQ 5 μm HPLC column (4.6 × 250 mm; Grace,
Deerfield, IL) using a linear acetonitrile gradient (0–10 min: 15 %;
10–30 min: 15% – 95%; 30–40 min: 95%;
40–45 min: 15%) with triethylammonium acetate (0.04 % v/v, pH 7.5)
as the mobile phase (Supplemental
Material, Figure S2 A–B). For the determination of the recovery of 4-PCB
11 sulfate and 3-F,4′PCB 3 sulfate (internal standard) from human serum samples, a
more rapid HPLC protocol (0–1 min: 15 % acetonitrile; 1–10 min:
15% – 95%; 10–14 min: 95%; 14–15 min:
15%) was applied with similar resolution. All separations were conducted at room
temperature using a Shimadzu LC-20-AT HPLC instrument and analytes were monitored by
absorbance measurements at 254 nm using a Shimadzu SPD-20-AT UV/VIS detector.
Grimm F.A., Lehmler H.J., Koh W.X., DeWall J., Teesch L.M., Hornbuckle K.C., Thorne P.S., Robertson L.W, & Duffel M.W. (2016). Identification of a sulfate metabolite of PCB 11 in human serum. Environment international, 98, 120-128.
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