Ab500

A549 and A549-taxolR cells were fixed, nuclei were isolated, and chromatin was sheared by sonication using the ChIP Kit according to the manufacturer’s instructions (ab500; Abcam). The sheared chromatin was immunoprecipitated using HSF1 antibody (ab-52757; Abcam) and IgG (ab500; Abcam). Details regarding the primers used in ChIP-qPCR can be found in Supplementary Table S2. The quantitative PCR conditions were as follows: 10 min at 95 °C, followed by 38 cycles of denaturation (5 s at 95 °C), annealing (10 s at 62 °C), and extension (20 s at 72 °C) with single acquisition of fluorescence at the end of each extension step. ChIP-qPCR was calculated as follows: fold enrichment = 2^{−((Ct IP)−(Ct mock))}. Mean values of three biological replicates were calculated. Statistical analysis was carried out using one-way ANOVA.

Chromatin immunoprecipitation (ChIP) assay was conducted using the ChIP kit (ab500, Abcam). First, equal amounts of cells were treated with 1% methanol for 10 min crosslink, washed with phosphate buffer saline, and resuspended in ChIP nuclear lysis buffer. Next, the crosslinked DNA was ultrasonically processed to generate chromatin fragments. After the lysates were clarified by centrifugation, the supernatant was incubated with the antibody against KLF9 (1:1000, 701888, Invitrogen) or immunoglobulin G (IgG; 1:100, ab6757, Abcam) at 4°C overnight. Subsequently, with an equal amount of pre‐clarified chromatins used as input, chromatins were incubated with fully resuspended protein A/G beads. After washing beads, immunocomplex was eluted by heating at 62°C and shaking. The crosslink of protein‐DNA complex was reversed after 2 h incubation. After purification, immunoprecipitated DNA was analyzed by PCR, with information of PCR primers shown in Table 1.