The human chondrocyte cell line CHON-001, derived from normal human articular cartilage, was obtained from American Type Culture Collection (ATCC; Manassas, Virginia) and cultured in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, New York), supplemented with 0.1 mg/ml G-418 (Gibco) and 10% fetal bovine serum (FBS; Gibco), in a humidified atmosphere with 5% CO2 at 37°C and passaged at a ratio of 1:5. The cell line was authenticated by Short Tandem Repeat (STR) profiling. The stable cultured CHON-001 cells were treated with recombinant human interleukin (IL)-1β (R&D Systems, Minneapolis, Minnesota) for 12 hours to stimulate cell inflammation. The following concentrations of IL-1β were used: 0.1 ng/ml, 2 ng/ml, 5 ng/ml, and 10 ng/ml.14 (link)-17 (link)
Chon 001
Manufactured by R&D Systems
CHON-001 is a laboratory instrument designed for the analysis of the elemental composition of samples. The device uses advanced analytical techniques to determine the presence and quantity of carbon, hydrogen, oxygen, and nitrogen within a given specimen.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Chon 001, by American Type Culture Collection (2 mentions)
Recombinant human interleukin il 1β, by R&D Systems (1 mentions)
Recombinant human il 1β, by R&D Systems (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
2 protocols using chon 001
1
Chondrocyte Inflammation Induction Protocol
2
Chondrocyte Inflammatory Injury Induction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human chondrocyte cell line CHON-001, derived from healthy human articular cartilage, was obtained from the American Type Culture Collection. Cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.), supplemented with 0.1 mg/ml G-418 (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), in a humidified atmosphere with 5% CO2 at 37°C and passaged at a ratio of 1:5.
The recombinant human IL-1β (R&D Systems, Inc.; 0.1, 2, 5 and 10 ng/ml) was used to treat the CHON-001 cells at 37°C for 12 h to induce cell inflammatory injury.
The recombinant human IL-1β (R&D Systems, Inc.; 0.1, 2, 5 and 10 ng/ml) was used to treat the CHON-001 cells at 37°C for 12 h to induce cell inflammatory injury.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!