Platinum atr model alpha

Affimers
were attached to the cubosomes by using DBCO-maleimide (Kerafast,
Inc., Boston, MA) click coupling chemistry. Briefly, 2 mL (0.5 mg/mL)
of Affimer clone II and clone III were reduced by using 150 μL
(5.7 mg/mL) of TCEP-HCl (Merck, Branchburg, NJ) for 90 min to remove
any dithiol-linked dimers. The reduced Affimers were incubated with
4 mM DBCO-maleimide for 2 h to attach DBCO, and then 100 mg/mL azide
containing cubosomes was added to allow click coupling to occur and
incubated for overnight at room temperature. The final product was
dialyzed for 24 h in 1x PBS by using Slide-A-Lyzer Dialysis Cassettes,
5K MWCO (Thermo Scientific, Waltham, MA) to remove unreacted Affimers.
FTIR spectroscopy (Platinum ATR, Model Alpha, Bruker, UK) was used
to confirm the covalent conjugation between the azide group of cubosomes
and the DBCO group attached to the Affimers.

FTIR analysis was performed after both aging methods by using Bruker platinum ATR model Alpha, Ettlingen, Germany with a spectral range of 4000–500 cm⁻¹. Absorbance values were obtained against wave number (cm⁻¹) by placing the sample directly on a diamond scanner.

The optical properties, surface morphology, thermal stability, elemental compositions and thickness of the MnS nanoparticles were evaluated through the following techniques: field emission scanning electron microscopy (FE-SEM, S-4200, Hitachi, Munich, Germany) coupled with energy dispersive X-ray spectroscopy (EDS) on-system, operating at a voltage of 15 kV. The size distributions of the three samples were identified using a JEOL JEM 2100 high-resolution transmission electron microscope (HRTEM, Pleasanton, CA, USA) operating at 200 kV. Their structural patterns were established using X-ray diffraction (XRD, Cambridge, UK) analysis with Cu Ka radiation at 40 mA and 40 kV, respectively. ThermoGravimetric Analyser 4000 (TGA Perkin Elmer, Johannesburg, South Africa) was performed at temperatures ranging from 30 to 600 °C at a rate of 10 °C min⁻¹. Fourier transform infrared spectroscopy (FTIR) analysis was achieved with the aid of a Bruker Platinum ATR Model Alpha (Waltham, MA, USA). PerkinElmer LAMBDA 365 and LS 45 fluorimeters (Waltham, MA, USA) were used to understand the optical properties (UV-Vis and PL analysis) of the three samples. NMR analysis was carried out using a Bruker AV-400 spectrometer (Waltham, MA, USA), working at 400.13 MHz and 300 K, with a spinning rate of 4 kHz.

Resultant synthesized materials were characterized by Fourier transform infrared spectroscopy (FTIR) using a Bruker platinum ATR model Alpha (Germany) spectrophotometer in the spectral range of 4000–750 cm−1 and X-ray diffraction analysis (XRD) using an X-ray diffractometer (D8 advance BRUKER) equipped with CuKα radiation source, having a wavelength of 0.154 nm and graphite monochromator in a 2θ range of 10°–80°. The morphology, size, and composition of synthesized materials were investigated via Scanning electron microscope (VEGA3 TESCAN) at an accelerating voltage of 20 kV. ¹H-NMR was carried out at a Bruker AVANCE III 500 MHz spectrometer. Mechanical properties of prepared blends were studied according to ASTM D882 by using Shimadzu Trapezium–X Universal Testing Machine (AG-20RRKNXD Plus). Test was carried out using dumbbell shaped samples of 20 mm × 10 mm dimensions. The extension rate was kept 10 mm/min. five samples of each film were tested at 27 °C.