We intraperitoneally injected 100 μL of luciferin substrate (15 mg/mL) (Biosynth). We performed optical imaging with the Lago optical imaging system (Spectral Instruments Imaging) 15 minutes later and analyzed the images with the Aura software from the same manufacturer.
Lago optical imaging system
Manufactured by Spectral Instruments Imaging
The Lago optical imaging system is a high-performance laboratory instrument designed for advanced imaging applications. It provides a reliable and precise platform for capturing and analyzing optical data. The core function of the Lago system is to facilitate the acquisition of high-quality images and data, enabling researchers and scientists to conduct their work effectively.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lago optical imaging system
1
Bioluminescent Imaging of Intraperitoneal Luciferin
2
In vivo fluorescence imaging protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For in vivo mKate2 and EGFP fluorescence expression analysis, animals were anesthetized with isoflurane prior to and during imaging. All fluorescence intensity measurements were acquired using a Lago optical imaging system (Spectral Instruments Imaging), which can image up to 10 animals simultaneously. For all analyses the following settings were used for image acquisition: mKate2 (ex. 570, em. 650), EGFP (ex. 465, em. 510), 20 s exposure at 15% power, and binning was set to 2 with a 25 or 20 cm field of view for in vivo and ex vivo tissue imaging, respectively. All fluorescence images were analyzed using Aura Software (Spectral Instruments Imaging) using the same box size.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!