Lolium temulentum L. (Lt, Darnel ryegrass) cv. Ceres seeds were planted, five seeds per pot, in TSD4 square pots (8.8 cm × 8.8 cm × 10 cm, 540 mL volume; McConkey Co., Sumner, WA) with Sun Gro Professional MM840 PC RSi (Sun Gro Horticulture, Hubbard, OR). Lolium plants were grown in Conviron PGR14 or PGR15 growth chambers (Conviron, Winnipeg, Canada), under 14 h photoperiods at 23 °C day and 18 °C night temperatures and fertilized with Technigro 20-18-20 all-purpose fertilizer (Sun Gro Horticulture, Hubbard, OR) weekly. All experiments were conducted using seeds from increases of Lolium temulentum cv. Ceres seeds originally provided by Dr. Lloyd T. Evans (CSIRO, Canberra Australia) in 2001.
Technigro 20 18 20 all purpose fertilizer
Manufactured by Sun Gro Horticulture
Sourced in Canada
Technigro 20-18-20 is an all-purpose fertilizer manufactured by Sun Gro Horticulture. It contains a balanced ratio of 20% nitrogen, 18% phosphorus, and 20% potassium, which are essential nutrients for plant growth and development.
Automatically generated - may contain errors
4 protocols using technigro 20 18 20 all purpose fertilizer
1
Growth Conditions for Darnel Ryegrass
2
Lolium temulentum Ceres Cultivation Protocol
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Lolium temulentum L. (Lt, Darnel ryegrass) cv. Ceres seeds were planted in 36 well flats, 5 cm × 3.8 cm × 5 cm well (1 plant/well) or 5 plants per pot TSD4 Square Height: 8.8 cm × 8.8 cm × 10 cm Volume: 540 ml (McConkey Co.) containing Sun Gro Professional MM840 PC RSi (Sun Gro Horticulture, Canada). Plants were grown under 12 h (RNA-Seq plants) or 14 h (qPCR plants) photo-periods at 23 °C day and 18 °C night in Conviron E15, PGR15, PGV36 or PDW40 (Conviron, Winnipeg, Canada) growth chambers. Plants were fertilized weekly using Technigro 20–18-20 all-purpose fertilizer (Sun Gro Horticulture, Canada). Lolium temulentum cv. Ceres seed was originally obtained from Dr. Lloyd T Evans (CSIRO, Canberra Australia) and all experiments were conducted using seeds increased from this original seed.
3
Darnel Ryegrass Growth Conditions
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Lolium temulentum L. (Darnel ryegrass) cv. Ceres seeds were planted in 2 × 1.5 × 2 inch pots (1 plant/pot) containing SB40 Sunshine Growing Mix (Sun Gro Horticulture, Canada). Plants were fertilized weekly using Technigro 20-18-20 all-purpose fertilizer (Sun Gro Horticulture, Canada). Plants were grown under 12 h photo-periods at 21°C d and 17°C night in Conviron E15 and PGV 36 (Conviron, Winnipeg, Canada) growth chambers.
4
Ceres Wheat Growth Protocol
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Seeds (five/pot) of Lt L. cv. Ceres were planted in 10.2 cm tall × 8.8 cm square pots (TSD4, McConkey Co., Sumner, WA, USA) containing Sungro Professional mix MM840 PC RSi (Agawan, MA, USA). Plants were grown in Conviron PGR14 or PGR15 chambers set to 14-h day (7 AM–9 PM) and day/night temperatures of 23 °C/18 °C (Figure 8 A) for 4–5 weeks until plants reached the 3-tiller stage. Plants were fertilized once/week with Technigro 20-18-20 all-purpose fertilizer (Sun Gro Horticulture, Hubbard, OR, USA).
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