Ht1080

Manufactured by Corning

The HT1080 is a laboratory equipment product developed by Corning. It is designed for use in research and clinical settings. The HT1080 is a compact, versatile device that can perform various tasks related to cell culture and tissue processing. Its core function is to provide a controlled environment for cell growth and analysis, but a detailed description of its intended use or capabilities is not available.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ht1080

Transwell Assay for Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell migration was performed with 5×10³ HT1080 cells or 5×10⁴ A431 cells plated in the top chamber of the Transwell insert on an uncoated membrane (8 μm pores for A431 and 2 μm pores for HT1080; Corning). Cells were seeded in the upper chamber in serum-free DMEM with the same concentration of inhibitors utilized for the proliferation assay; 10% FBS-containing DMEM was the chemoattractant in the lower chamber. Cells were allowed to migrate for 18-24 h then rinsed in PBS, fixed in paraformaldehyde and stained with 0.5% crystal violet in 50% methanol. Unmigrated cells in the upper chambers were removed with a cotton swab, and migrated cells in the lower chambers were imaged in 5 random fields using an inverted microscope (EVOS, Life Technologies, Grand Island, NY).

Overmiller A.M., McGuinn K.P., Roberts B.J., Cooper F., Brennan-Crispi D.M., Deguchi T., Peltonen S., James K. W I.I.I, & Mahoney M.G. (2016). c-Src/Cav1-dependent activation of the EGFR by Dsg2. Oncotarget, 7(25), 37536-37555.

+ Open protocol

+ Expand

Cell Culture and Transfection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK 293 T (CRL-11268), HeLa (CCL-2), MCF7 (HTB-22), HT1080 (CCL-121), and Neuro-2a (CCL-131) cells were purchased from American Type Culture Collection (ATCC). HEK 293 T, HeLa, and Neuro-2a cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Corning) at 37°C with 10% or 5% CO₂ under standard humidity. MCF7 and HT1080 cells were cultured in Eagle’s minimum essential medium with 10% FBS (Corning) at 37°C with 10% CO₂ under standard humidity. The cells were transfected using Lipofectamine LTX (Invitrogen) according to the manufacturer’s recommendations.

Yu J., Shin J., Yu J., Kim J., Yu D, & Heo W.D. (2024). Programmable RNA base editing with photoactivatable CRISPR-Cas13. Nature Communications, 15, 673.

+ Open protocol

+ Expand

Cell Culture Protocol for Various Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MM.1S (#CRL-2974), H929 (#CRL-9068), Toledo (#CRL-2631), THP-1 (TIB-202), A549 (CCL-185), HT1080 (#CCL-121), U-2 OS (#HTB-96), MiaPaCa-2 (#CRL-1420), MDA-MB-231 (HTB-26) were purchased from ATCC (Manassas, VA, USA), MOLM13 cells (#ACC554) were purchased from DSMZ (Braunschweig, Germany) and ASPS-KY (Hoshino et al. 2009) were obtained with the permission of Dr. Shunsuke Yanoma. MM.1S, H929, Toledo, THP-1, ASPS-KY and MDA-MB-231 cells were cultured in RPMI-1640 medium (#10-040-CV; Corning). A549 cells were cultured in F12-K medium (#21127-022; Gibco). MiaPaCa-2 cells were cultured in Dulbecco's Modified Eagle's medium (#10-013-CV; Corning). HT1080 cells were cultured in Eagle's Minimum Essential medium (#10-010-CV; Corning). U-2 OS cells were cultured in McCoy's 5A medium (#12330-031; Gibco). Cell media was supplemented with 10% fetal bovine serum, penicillin 10,000 U/mL, and streptomycin 10,000 μg/mL (#15140-122; Gibco).

Kashyap T., Argueta C., Unger T., Klebanov B., Debler S., Senapedis W., Crochiere M.L., Lee M.S., Kauffman M., Shacham S, & Landesman Y. (2018). Selinexor reduces the expression of DNA damage repair proteins and sensitizes cancer cells to DNA damaging agents. Oncotarget, 9(56), 30773-30786.

+ Open protocol

+ Expand

Cell Line Cultivation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human chronic myelogenous leukemia cell line HAP1 was purchased from Horizon Discovery. Human embryonic kidney cell line 293T, human osteosarcoma cell line U2OS, human proximal tubular cell line HK2, human lung fibroblast cell line MRC-5, human cervical adenocarcinoma cell line HeLa, human colorectal carcinoma cell line HCT116, human mesothelioma cell line H226, human fibrosarcoma cell line HT1080, and human hepatocellular carcinoma cell line HepG2 were obtained from American Type Culture Collection. These cell lines were cultured in respective medium: Iscove’s Modified Dulbecco’s Medium for HAP1, Dulbecco’s modified Eagle’s medium for 293T and HeLa, McCoy’s 5A medium for U2OS and HCT116, Eagle’s minimum essential medium for MRC-5, HT1080, and HepG2. All of these media were supplemented with 10% fetal bovine serum (FBS). For HT1080 and HepG2, the medium was also supplemented with MEM non-essential amino acids (Corning) and sodium pyruvate (Corning). HK2 cell line was cultured in keratinocyte serum free medium (Invitrogen).

Kojima Y., Machida Y., Palani S., Caulfield T.R., Radisky E.S., Kaufmann S.H, & Machida Y.J. (2020). FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Nature Communications, 11, 1318.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!