Mouse adiponectin acrp30 duoset

Lactoferrin (90.5% pure with 16% iron saturation) was obtained from Westland Milk Products (Hokitika, New Zealand). Double-antibody sandwich ELISA kits to identify TNF-α, IL-1β, IL-4, IL-6, IL-13, IL-33, MCP-1, and TSLP (ELISA Ready-SET-Go; eBioscience, San Diego, CA, USA) were purchased. In addition, adiponectin (Mouse Adiponectin/Acrp30 DuoSet; R&D Systems Inc., Minneapolis, MN, USA), insulin (Ultrasensitive Mouse Insulin ELISA; Mercodia Inc., Winston, Salem, NC, USA), endotoxin (lipopolysaccharide) (ToxinSensor Chromogenic LAL Endotoxin Assay Kit; GenScript USA Inc., Piscataway, NJ, USA), and bovine lactoferrin (Bethyl Laboratories Inc., Montgomery, TX, USA) ELISA kits were purchased and according to the instruction to detect serum or hepatic homogenate.

At D5, following differentiation, the conditioned medium was collected and adiponectin concentration in conditioned medium was measured using the Mouse Adiponectin/Acrp30 DuoSet (R&D Systems, Minneapolis, MN, USA).