Boilogic mos 500 spectrometer

Optical rotations were measured on an Anton paar MCP 5100 modular circular polarimeter (JASCO, Tokyo, Japan). ECD spectra were recorded on a Boilogic Mos-500 spectrometer (JASCO, Tokyo, Japan). IR spectra were recorded on a Nicolet 6700 spectrophotometer (Thermo Scientific, Madison, WI, USA). UV spectra were recorded on a Beckman DU 640 spectrophotometer (JASCO, Tokyo, Japan). The 1D and 2D NMR spectra were obtained with a Bruker AV spectrometer (400 MH_Z for ¹H and 100 MH_Z for ¹³C, (Bruker Corporation, Basel, Switzerland) or a JNM-ECZS spectrometer (600 HM_Z for ¹H and 125 MH_Z for ¹³C, (JEOL, Tokyo, Japan), using Methanol-d₄ or DMSO-d₆ as a solvent. TMS was used as an internal standard. HR-ESI-MS spectra were measured on a Bruker APEX II spectrometer (Billerica, MA, USA). Silica gel (Qing Dao Hai Yang Chemical Group Co., Qingdao, China; 200–300 mesh) and octadecylsilyl silica gel (YMC; 12 μm–50 μm) were used for column chromatography (CC). Precoated silica gel plates (Yan Tai Zi Fu Chemical Group Co., Yan Tai, China; G60, F-254) were used for thin layer chromatography (TLC). Semi-preparative HPLC was performed on an Agilent 1260 LC series with a DAD detector using an Agilent Eclipse XDB-C₁₈ column (250 × 9.4 mm, 5 μm, Agilent Corporation, Santa Clara, CA, USA).

Optical rotations were acquired by an Anton paar MCP 5100 modular circular polarimeter (JASCO, Tokyo, Japan). CD spectra and UV spectra were obtained from a Boilogic Mos-500 spectrometer (JASCO, Tokyo, Japan). The 1D and 2D NMR (COSY, HSQC, and HMBC) spectra were recorded on a Bruker AV spectrometer (400 MHz for ¹H and 100 MHz for ¹³C, (Bruker Corporation, Switzerland) instrument using DMSO-d₆ as a solvent. Tetramethyl silane (TMS) was used as an internal standard. HR-ESI-MS spectra were made on a Bruker APEX II spectrometer (Billerica, MA, USA). Semi preparative HPLC was carried out with Agilent 1260 prep-HPLC system, using Agilent Eclipse XDB-C18 (9.4 × 250 mm, 5 μm, Agilent Corporation, Santa Clara, CA, USA), respectively. Silica gel (200–300 mesh, 300–400 mesh Qingdao Marine Chemical Factory, Qingdao, China) and octadecylsilyl silica gel (YMC; 12–50 μm) were used for column chromatography (CC). Precoated silica gel plates (GF-254, Qingdao Marine Chemical Factory, Qingdao, China) were used for thin layer chromatography (TLC). All solvents used for extractions and chromatographic separations were of analytical grade and purchased from Xilong Chemical Reagent Factory (Guangzhou, China), with the exception of HPLC grade solvents used for HPLC separations.