The as-prepared herba artemisiae scopariae (HAS) material was extracted with NADES using a water bath. In order to prevent splashing, the dried materials were infiltrated for 5 min by adding 5 ml of water until the HAS were completely moist. 2 g of dried HAS particles were mixed with 10 mL of the as-prepared NADESs and conventional solvents (water and ethanol, respectively) in flasks, and the samples were pre-treated via a process of ultrasonic cell smashing under 300 W for 30 min. The active substances were released adequately. Then, the flasks were placed in water baths at 323 K with continuous stirring for 2 h, and the crude extract was obtained through a filter to remove the dregs. Next, the turbid extracts were centrifuged at 1400 rpm for 20 min to remove the finer impurities. The supernatant was further applied to complete the determination of the chlorogenic acid content using an Agilent 1260 high-performance liquid chromatography instrument. The parallel test repeated three times for average. The extraction rate was calculated using the formula: where C is the concentration of chlorogenic acid measured under HPLC conditions, V is the sample volume, n is the dilution factor, m is the weight of HAS.
Agilent 1260 high
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 1260 high is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design and advanced technologies to deliver reliable and accurate results. The core function of the Agilent 1260 high is to separate and analyze complex mixtures of chemical compounds.
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Lab products found in correlation
Diode array detector, by Agilent Technologies (1 mentions)
Eclipse plus c18, by Agilent Technologies (1 mentions)
Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)
Agilent zorbax sb c18, by Agilent Technologies (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
Ix71 optical microscope, by Olympus (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Jem 1400 transmission electron microscope, by JEOL (1 mentions)
7 protocols using agilent 1260 high
1
Extraction of Chlorogenic Acid from Herba Artemisiae Scopariae
2
HPLC Analysis of Chemical Compounds
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Agilent 1260 high-performance liquid chromatography (HPLC) with an auto-injector and a diode array detector came from Agilent Technologies Inc. (Santa Clara, CA, USA). Chromatographic separations were achieved on the Agilent Eclipse Plus C18 column (250 × 4.6 mm, 5 μm). The injection volume was 20 μL. The mobile phase was methanol + water (4 + 1 by volume) at a flow rate of 0.5 mL min−1. The column temperature was held at 20 °C. The detection wavelength was 220 nm. The HPLC chromatogram was presented in Fig. S1.†
3
Diosgenin Quantification in RDN Using HPLC
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The RDN test sample was prepared according to a previous study [33 (link)]. Standard diosgenin was purchased from the Tauto Biological Technology Company (China). The chemical constituents of RDN were determined using an Agilent 1260 high-performance liquid chromatography (HPLC) system (Agilent, CA, USA). Samples (10 μL) were separated using an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm × 250 mm, 5 μm) at 30°C, with mobile phases consisting of water (A) and acetonitrile (B). The following gradient conditions were used: 0–5 min, 15% B; 5–30 min, 15–40% B; and 30–50 min, 40–50% B. The flow rate was 1.0 mL/min, and the detection wavelength was 203 nm. Chromatograms are shown in Supplementary Figure 1 . The retention time of diosgenin was 7.439 min.
4
Melting Point and Spectroscopic Analysis
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Melting points were determined in WRS-2 melting point apparatus (Shanghai Precision & Scientific Instrument Co., Ltd., Shanghai, China) and were uncorrected. IR spectra were recorded on a Nicolet IS10 FT-IR spectrometer (Nicolet, Madison, WI, USA). 1H NMR and 13C NMR spectra were recorded on a Bruker DKX500 NMR spectrometer (Bruker, Karlsruhe, Germany) using CDCl3 as solvent, and TMS as internal standard. ESI-MS were recorded on a Waters Q-TOF MicroTM mass spectrometer. Purity of compounds was detected by Agilent 1260 high performance liquid chromatography (Agilent, Santa Clara, CA, USA) and Fuli GC-9750 gas chromatography (Zhejiang Fuli analysis instrument Co. Ltd., Wenling, China). All reactions were traced by the thin layer chromatography (TLC). The compound (–)-β-pinene was purchased from the spice company Jiangxi Jishui Hongda Natural Perfume Co., Limited, Ji’an, China, and other reagents were of reagent grade. Fungi were isolated by the plant pathology laboratory in the College of Agriculture, Jiangxi Agricultural University.
5
Determination of Bioactive Compounds in Coptis Species
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0.2 g dried powder of two Coptis leaves, roots and rhizomes was respectively extracted with 50 mL of hydrochloric acid-methanol mixed liquor (1:100, v/v) for 30 min, and sonicated for 30 min. For determining the main bioactive components of two Coptis species, an Agilent 1260 High-performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA, United States) was used. Chromatographic separation was performed on the chromatographic column Agilent Zorbax SB-C 18 (250 mm × 4.6 mm, 5 μm, Agilent Technologies) at a column temperature of 30°C, the flow rate was fixed at 1 mL/min. The mobile phase consisted of acetonitrile-0.05mol/L potassium dihydrogen phosphate solution (50:50, v/v) containing 0.1% sodium dodecyl sulfate, and separation was achieved by an isocratic elution. Detection was performed at 345 nm (Zhang G.H. et al., 2015 (link); He et al., 2017 (link)). The content of berberine, coptisine, jatrorrhizine, palmatine, and epiberberine were calculated from standard curves. Authentic berberine, palmatine, jatrorrhizine, coptisine and epiberberine were purchased from JK chemical (Beijing, China).
6
SPE-HPLC Analysis of Water Samples
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Raw water, osmotic solution, and residual water were collected and filtered by a 0.45 μm filter membrane to remove suspended particles. An HLB column was used for SPE water sample pretreatment. The samples were determined by Agilent 1260 high-performance liquid chromatography (Santa Clara, CA, USA). The chromatographic column was ZORBAX Eclipse XDB-C18. The mobile phase consisted of acetonitrile and ultra-pure water with a pH of 3 regulated by phosphoric acid.
7
Characterization of G250-Loaded Nanobubbles
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The distribution of G250-TNBs was observed using an IX71 optical microscope (Olympus Corporation, Kyoto, Japan), and the concentration was measured using a hematocytometer. Morphological characteristics were observed using a JEM-1400 transmission electron microscope (JEOL, Tokyo, Japan). The mean particle size, polydispersity index (PDI), and zeta potential were measured using a Malvern Zetasizer Nano ZS90 (Malvern Instruments Inc., Worcestershire, UK). The G250-TNBs were observed and measured daily over a period of 1 week to evaluate changes in concentration and particle diameter with time (during this time, they were stored at 4°C). The encapsulation efficiency (EE) and loading efficiency (LE) of G250-TNBs were measured by an Agilent 1260 high-performance liquid chromatography (HPLC) (Agilent Technologies, Santa Clara, CA, USA). The chromatographic conditions were as follows: chromatographic column: Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 3 μm); column temperature: 30°C; flow rate: 0.8 mL/min; injection volume: 10 μL; mobile phase: acetonitrile:0.5% glacial acetic acid (45:55); detection wavelength: 277 nm. EE = amount of TEM encapsulated in NBs/total amount of added TEM × 100%; LE = amount of TEM encapsulated in NBs/total amount of lipids for NB preparation × 100%.15 (link)
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