Pro (Plofed 1%, Polfa S.A., Poland), Quin (Accupro, Pfizer, Germany), lipofundin (MCT/LCT 10%, Braun, Germany), gum arabic (Pharma Cosmetic, Poland), pentobarbital (Vetbutal, Biovet, Poland), collagen type I (Collagen, Chronolog, USA), and heparin (Heparinum, Polfa, Poland) were used in the study. Bovine albumin, apyrase, HEPES (N-(2-hydroxyethyl)piperazine-N′-(2-ethanosulfonic acid) were delivered by Sigma-Aldrich (Poland). Calcium chloride, EDTA, glucose, magnesium chloride, sodium chloride, potassium chloride, sodium bicarbonate, sodium phosphate, Tris buffer, and trisodium citrate were provided by Polish Chemicals Reagents (Poland). Rabbit anti-rat TAFI monoclonal antibody (ImmunoKontact AMS Biotechnology, Germany), rat active PAI-1 ELISA kit (Innovative Research, USA), rat active t-PA ELISA kit (Innovative Research, USA), hydrogen peroxide colorimetric detection kit (Assay Designs, USA), correlate assay nitric oxide NO-2/NO-3 assay kit (Assay Designs, USA), MDA adducts ELISA kit (Cell Biolabs, USA), Trizol (Invitrogen Life Technologies, USA), qPCRTM Mastermix, SYBR Green I (Eurogentec Seraing, Belgium), Oligotex Kit (Qiagen, USA), and TaqMan reverse transcription reagents kit (Applied Biosystems, USA) were also used.
Rat active t pa elisa kit
Manufactured by Innovative Research
Sourced in United States
The Rat Active t-PA ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of rat tissue plasminogen activator (t-PA) levels in biological samples. The kit utilizes a specific antibody coated on a microplate to capture the target analyte from the sample. A detection antibody conjugated with an enzyme is then used to quantify the captured t-PA. The assay provides a reliable and reproducible method for determining the concentration of active t-PA in rat samples.
Automatically generated - may contain errors
Lab products found in correlation
Rat active pai 1 elisa kit, by Innovative Research (3 mentions)
Scil vet abc plus, by Horiba (2 mentions)
Bovine albumin, by Merck Group (1 mentions)
Apyrase, by Merck Group (1 mentions)
Mda adducts elisa kit, by Cell Biolabs (1 mentions)
Oligotex kit, by Qiagen (1 mentions)
Taqman reverse transcription reagents kit, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Hepes, by Merck Group (1 mentions)
Collagen type 1, by Chrono-Log (1 mentions)
Elx808, by Agilent Technologies (1 mentions)
3 protocols using rat active t pa elisa kit
1
Evaluation of Hemostasis and Oxidative Stress
2
Blood Morphology and Coagulation Factors
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Blood morphology test was performed using a hematological analyzer (ScilVet ABC Plus+, HORIBA ABX, Grabels, France). Plasma levels of tissue factor (TF), tissue plasminogen activator (t-PA), and plasminogen activator inhibitor type 1 (PAI-1) were measured by enzyme immunoassays (Rabbit Monoclonal Antibody Anti Rat TF; ImmunoKontact AMS Biotechnology, Abingdon, UK; Rat Active tPA ELISA Kit, Innovative Research, Novi, MI, USA; Rat Active PAI-1 ELISA Kit; Innovative Research, Novi, MI, USA, respectively).
3
Plasma Coagulation Factors Determination
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Tissue factor, t-PA and PAI-1 plasma levels were determined by enzyme immunoassay (Rat Tissue Factor ELISA Kit, MyBioSource, Inc., San Diego, CA, USA; Rat Active t-PA ELISA Kit and Rat Active PAI-1 ELISA Kit, Innovative Research, Inc., Novi, MI, USA) in a microplate reader (ELx808, BioTek Instruments, Inc., Winooski, VT, USA) according to the manufacturer’s instructions. A blood morphology test was carried out using the hematological analyzer ScilVet ABC Plus+ (HORIBA ABX, Montpellier, France). A volumetric impedance method of counting blood cells was applied [54 (link)] for determination of white blood cells (WBCs), red blood cells (RBCs), medium cell volume (MCV), medium content of hemoglobin (MCH), medium cell hemoglobin concentration (MCHC), hemoglobin (HGB), platelet count (PLT), as well as automatic calculation of hematocrit (HCT).
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